Membrane recognition and dynamics of the RNA degradosome. [PDF]
PLoS Genetics, 2015 RNase E, which is the central component of the multienzyme RNA degradosome, serves as a scaffold for interaction with other enzymes involved in mRNA degradation including the DEAD-box RNA helicase RhlB.
Henrik Strahl +10 more
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Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediates. [PDF]
PLoS Biology, 2023 RNA processing and degradation shape the transcriptome by generating stable molecules that are necessary for translation (rRNA and tRNA) and by facilitating the turnover of mRNA, which is necessary for the posttranscriptional control of gene expression ...
Lydia Hadjeras +10 more
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The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen Helicobacter pylori. [PDF]
MBio, 2020 International audiencePosttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be ...
Hilde de Reuse +2 more
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The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome [PDF]
Bioscience Reports, 2014 Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing.
Feng Lu, Aziz Taghbalout
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Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components [PDF]
Nature Communications, 2018 The mitochondrial RNA degradosome (mtEXO) plays an essential role in the regulation of mitochondrial gene expression and is composed of the 3′-to-5′ exoribonuclease Dss1 and the helicase Suv3.
Michal Razew +12 more
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Structural elucidation of a novel mechanism for the bacteriophage-based inhibition of the RNA degradosome [PDF]
eLife, 2016 In all domains of life, the catalysed degradation of RNA facilitates rapid adaptation to changing environmental conditions, while destruction of foreign RNA is an important mechanism to prevent host infection.
An Van den Bossche +11 more
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Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]
Open Biology, 2012 Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick +4 more
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Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation [PDF]
Nature CommunicationsIn many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that
Johann J. Roske +6 more
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Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa. [PDF]
PLoS GeneticsThe RNA degradosome is a bacterial multi-protein complex mediating mRNA processing and degradation. In Pseudomonadota, this complex assembles on the C-terminal domain (CTD) of RNase E through short linear motifs (SLiMs) that determine its composition and
Sandra Amandine Marie Geslain +5 more
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Mitochondrial RNA degradation regulates differentiation, stemness, and immune sensitivity in acute myeloid leukemia [PDF]
Nature CommunicationsEukaryotic cells have separate genomes in the nucleus and mitochondria. Mitochondrial DNA is transcribed bi-directionally to generate mitochondrial RNA (mtRNA) and dsRNA as a by-product of this transcription.
Geethu Emily Thomas +18 more
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