The Structure and Function of the Gram-Positive Bacterial RNA Degradosome. [PDF]
Front Microbiol, 2017 The RNA degradosome is a highly structured protein complex responsible for bulk RNA decay in bacteria. The main components of the complex, ribonucleases, an RNA helicase, and glycolytic enzymes are well-conserved in bacteria. Some components of the degradosome are essential for growth and the disruption of degradosome formation causes slower growth ...
Cho KH.
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The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA. [PDF]
Proc Natl Acad Sci U S A, 2019 The factors and mechanisms that govern tRNA stability in bacteria are not well understood. Here, we investigated the influence of posttranscriptional modification of bacterial tRNAs (tRNA modification) on tRNA stability. We focused on ThiI-generated 4-thiouridine (s 4 U), a modification found in bacterial and ...
Kimura S, Waldor MK.
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Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. [PDF]
J Mol Biol, 2008 The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors.
Worrall JA +7 more
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An RNA degradosome assembly in Caulobacter crescentus. [PDF]
Nucleic Acids Res, 2011 In many bacterial species, the multi-enzyme RNA degradosome assembly makes key contributions to RNA metabolism. Powering the turnover of RNA and the processing of structural precursors, the RNA degradosome has differential activities on a spectrum of transcripts and contributes to gene regulation at a global level.
Hardwick SW +3 more
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The proteomic response to mutants of the Escherichia coli RNA degradosome. [PDF]
Mol Biosyst, 2013 The Escherichia coli RNA degradosome recognizes and degrades RNA through the coordination of four main protein components, the endonuclease RNase E, the exonuclease PNPase, the RhlB helicase and the metabolic enzyme enolase. To help our understanding of the functions of the RNA degradosome, we quantified expression changes of >2300 proteins using mass ...
Zhou L +4 more
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Crystal structure of the Escherichia coli RNA degradosome component enolase [PDF]
Journal of Molecular Biology, 2001 The crystal structure of Escherichia coli enolase (EC 4.2.1.11, phosphopyruvate hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A. There are four molecules in the asymmetric unit of the C2 cell, and in one of the molecules, flexible loops close onto the active site.
Kühnel, K., Luisi, B.
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The small heat-shock proteins IbpA and IbpB reduce the stress load of recombinant
Microbial Cell Factories, 2005 Background The permanently impaired protein folding during recombinant protein production resembles the stress encountered at extreme temperatures, under which condition the putative holding chaperones, IbpA/IbpB, play an important role. We evaluated the
Hoffmann Frank +2 more
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Structure of the 30S translation initiation complex coupled to paused RNA polymerase and its potential for riboregulation [PDF]
Nature CommunicationsIn many bacterial species, transcription and translation can be coupled physically, with potential impact on the rates and efficiency of gene expression. Here, we present structural evidence from cryo-EM demonstrating that a bacterial RNA polymerase that
Johann J. Roske +6 more
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Human mitochondrial degradosome prevents harmful mitochondrial R loops and mitochondrial genome instability [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2018 R loops are nucleic acid structures comprising an DNA-RNA hybrid and a displaced single-stranded DNA. These structures may occur transiently during transcription, playing essential biological functions. However, persistent R loops may become pathological
Aguilera López, Andrés +2 more
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Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa. [PDF]
PLoS GeneticsThe RNA degradosome is a bacterial multi-protein complex mediating mRNA processing and degradation. In Pseudomonadota, this complex assembles on the C-terminal domain (CTD) of RNase E through short linear motifs (SLiMs) that determine its composition and
Sandra Amandine Marie Geslain +5 more
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