Results 21 to 30 of about 738,204 (145)
Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome. [PDF]
J Mol Biol, 2008 The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular ...
Worrall JA +7 more
europepmc +2 more sources
Pakistan Journal of Pharmaceutical Sciences, 2021 Neisseria meningtidis is responsible for causing meningococcal meningitis along with acute septicaemia in human beings. Functional genomics strategies proved cruciality of certain genes/proteins in Neisseria meningitidis pathogenesis.
Mehwish Hamid +4 more
doaj +1 more source
Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]
, 2010 For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core +3 more sources
The RNase J-Based RNA Degradosome Is Compartmentalized in the Gastric Pathogen
mBio, 2020 Posttranscriptional regulation is a major level of gene expression control in any cell. In bacteria, multiprotein machines called RNA degradosomes are central for RNA processing and degradation, and some were reported to be compartmentalized inside these
Alejandro Tejada-Arranz +5 more
doaj +1 more source
A multi-dentate, cooperative interaction between endo- and exo-ribonucleases within the bacterial RNA degradosome. [PDF]
Nucleic Acids ResIn Escherichia coli and numerous other bacteria, two of the principal enzymes mediating messenger RNA decay and RNA processing-RNase E, an endoribonuclease, and polynucleotide phosphorylase (PNPase), an exoribonuclease-assemble into a multi-enzyme ...
Paris G +6 more
europepmc +3 more sources
Human FASTK preferentially binds single-stranded and G-rich RNA. [PDF]
FEBS JAlthough FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Dawidziak DM +7 more
europepmc +2 more sources
Localization of components of the RNA-degrading machine in Bacillus subtilis
Frontiers in Microbiology, 2016 In bacteria, the control of mRNA stability is crucial to allow rapid adaptation to changing conditions. In most bacteria, RNA degradation is catalyzed by the RNA degradosome, a protein complex composed of endo- and exoribonucleases, RNA helicases and ...
Nora Cascante-Estepa +2 more
doaj +1 more source
mBio, 2022 The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra +4 more
doaj +1 more source
Towards Understanding Helicase and Chaperone Activities in the RNA Degradosome [PDF]
, 2020 The E. coli RNA degradosome is a complex multi-enzyme machine which is central to the post-transcriptional regulation of the cell. Some of its functions include maturing and processing sRNA, rRNA, and tRNA, as well as degrading mRNA.
core +2 more sources
LEVELNET to visualize, explore, and compare protein–protein interaction networks
PROTEOMICS, Volume 23, Issue 17, September 2023., 2023 Abstract Physical interactions between proteins are central to all biological processes. Yet, the current knowledge of who interacts with whom in the cell and in what manner relies on partial, noisy, and highly heterogeneous data. Thus, there is a need for methods comprehensively describing and organizing such data.
Yasser Mohseni Behbahani +4 more
wiley +1 more source