Results 21 to 30 of about 3,502 (177)

Association of the Cold Shock DEAD-Box RNA Helicase RhlE to the RNA Degradosome in Caulobacter crescentus. [PDF]

J Bacteriol, 2017
ABSTRACT In diverse bacterial lineages, multienzyme assemblies have evolved that are central elements of RNA metabolism and RNA-mediated regulation. The aquatic Gram-negative bacterium Caulobacter crescentus , which has been a model system for studying the bacterial cell cycle, has an RNA degradosome ...
Aguirre AA, Vicente AM, Hardwick SW, Alvelos DM, Mazzon RR, Luisi BF, Marques MV.   +6 more
europepmc   +3 more sources

Molecular recognition of RhlB and RNase D in the Caulobacter crescentus RNA degradosome. [PDF]

Nucleic Acids Res, 2014
The endoribonuclease RNase E is a key enzyme in RNA metabolism for many bacterial species. In Escherichia coli, RNase E contributes to the majority of RNA turnover and processing events, and the enzyme has been extensively characterized as the central component of the RNA degradosome assembly.
Voss JE, Luisi BF, Hardwick SW.
europepmc   +5 more sources

Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli. [PDF]

Nucleic Acids Res, 2012
The RNA degradosome is a multi-enzyme assembly that contributes to key processes of RNA metabolism, and it engages numerous partners in serving its varied functional roles. Small domains within the assembly recognize collectively a diverse range of macromolecules, including the core protein components, the cytoplasmic lipid membrane, mRNAs, non-coding ...
Tsai YC, Du D, Du D, Domínguez-Malfavón L, Dimastrogiovanni D, Cross J, Callaghan AJ, García-Mena J, Luisi BF.   +8 more
europepmc   +6 more sources

The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome. [PDF]

Biosci Rep, 2014
Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing. In addition to the degradosome complex, the cell contains other ribonucleases that also play important roles ...
Lu F, Taghbalout A.
europepmc   +4 more sources

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]

Open Biology, 2012
Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick, Tobias Gubbey, Isabelle Hug, Urs Jenal, Ben F. Luisi   +4 more
doaj   +5 more sources

Studies of the RNA Degradosome-organizing Domain of the Escherichia coli Ribonuclease RNase E [PDF]

Journal of Molecular Biology, 2004
The hydrolytic endoribonuclease RNase E, which is widely distributed in bacteria and plants, plays key roles in mRNA degradation and RNA processing in Escherichia coli. The enzymatic activity of RNase E is contained within the conserved amino-terminal half of the 118 kDa protein, and the carboxy-terminal half organizes the RNA degradosome, a multi ...
Callaghan, Anastasia, Aurikko, J., Ilag, L., Gunter Grossmann, J., Chandran, V., Kuhnel, K., Poljak, L., Carpousis, A., Robinson, Carol V., Symmons, M., Luisi, B.   +10 more
openaire   +6 more sources

Formation of a membraneless compartment regulates bacterial virulence [PDF]

Nature Communications
The RNA-binding protein CsrA regulates the expression of hundreds of genes in several bacterial species, thus controlling virulence and other processes.
Lior Aroeti, Netanel Elbaz, Raya Faigenbaum-Romm, Oren Yakovian, Yael Altuvia, Liron Argaman, Naama Katsowich, Michal Bejerano-Sagie, Miriam Ravins, Hanah Margalit, Sigal Ben-Yehuda, Ilan Rosenshine   +11 more
doaj   +2 more sources

Mechanistic insights into recruitment and regulation of the RNA helicase UPF1 in replication-dependent histone mRNA decay [PDF]

Nature Communications
Metazoan histone mRNAs are a unique class of mRNAs that lack the poly(A) tail present in all other eukaryotic transcripts. Instead, they end in a conserved stem-loop (SL) structure, necessitating a decay mechanism that is distinct from deadenylation ...
Alexandrina Machado de Amorim, Guangpu Xue, Wenxia He, Theresa Dittmers, Sarah Lewandowski, Cecilia Perez-Borrajero, Juliane Bethmann, Nevena Mateva, Clemens Krage, Vidhyadhar Nandana, Bernhard Loll, Tarek Hilal, Janosch Hennig, Henning Urlaub, William F. Marzluff, Sutapa Chakrabarti   +15 more
doaj   +2 more sources

Proteomic and transcriptomic experiments reveal an essential role of RNA degradosome complexes in shaping the transcriptome of Mycobacterium tuberculosis. [PDF]

Nucleic Acids Res, 2019
Płociński P, Macios M, Houghton J, Niemiec E, Płocińska R, Brzostek A, Słomka M, Dziadek J, Young D, Dziembowski A.   +9 more
europepmc   +2 more sources

Polyribosome-Dependent Clustering of Membrane-Anchored RNA Degradosomes To Form Sites of mRNA Degradation in Escherichia coli

mBio, 2021
Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche, Leonora Poljak, Agamemnon J. Carpousis   +2 more
doaj   +1 more source