Results 41 to 50 of about 4,003 (181)

A cooperative PNPase-Hfq-RNA carrier complex facilitates bacterial riboregulation

Molecules and Cells, 2020
Summary Polynucleotide phosphorylase (PNPase) is an ancient exoribonuclease conserved in the course of evolution and is found in species as diverse as bacteria and humans. Paradoxically, Escherichia coli PNPase can act not only as an RNA degrading enzyme
T. Dendooven, D. Sinha, Alžběta Roeselová, T. A. Cameron, Nicholas R. De Lay, B. Luisi, K. Bandyra   +6 more
semanticscholar   +1 more source

Suppression of PNPase Alleviates Gestational Diabetes Mellitus-cardiovascular Injury through Up-regulating MicroRNA-26a and Down-regulating PTEN

, 2021
Objective: Gestational diabetes mellitus (GDM) is often accompanied by cardiovascular injury (CI), while the specific pathology remains largely unknown.
Li Liu, Haiying Wu, Xianghong Cheng
semanticscholar   +1 more source

Pseudomonas aeruginosa Polynucleotide Phosphorylase Contributes to Ciprofloxacin Resistance by Regulating PrtR

Frontiers in Microbiology, 2019
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that causes various acute and chronic infections. It is intrinsically resistant to a variety of antibiotics. However, production of pyocins during SOS response sensitizes P.
Zheng Fan, Hao Chen, Mei Li, Xiaolei Pan, Weixin Fu, Huan Ren, Ronghao Chen, Fang Bai, Yongxin Jin, Zhihui Cheng, Shouguang Jin, Weihui Wu   +11 more
doaj   +1 more source

Critical roles for ‘housekeeping’ nucleases in type III CRISPR-Cas immunity

eLife, 2022
CRISPR-Cas systems are a family of adaptive immune systems that use small CRISPR RNAs (crRNAs) and CRISPR-associated (Cas) nucleases to protect prokaryotes from invading plasmids and viruses (i.e., phages).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj   +1 more source

Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly [PDF]

Open Biology, 2012
Polynucleotide phosphorylase (PNPase) is an exoribonuclease that cleaves single-stranded RNA substrates with 3′–5′ directionality and processive behaviour.
Steven W. Hardwick, Tobias Gubbey, Isabelle Hug, Urs Jenal, Ben F. Luisi   +4 more
doaj   +1 more source

Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis. [PDF]

FEBS Lett
The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Gilet L, Leroy M, Maes A, Condon C, Braun F.   +4 more
europepmc   +2 more sources

Oocyte Factors Suppress Mitochondrial Polynucleotide Phosphorylase to Remodel the Metabolome and Enhance Reprogramming

Cell Reports, 2015
Oocyte factors not only drive somatic cell nuclear transfer reprogramming but also augment the efficiency and quality of induced pluripotent stem cell (iPSC) reprogramming.
Swea-Ling Khaw, Chua Min-Wen, Cheng-Gee Koh, Bing Lim, Ng Shyh-Chang   +4 more
doaj   +1 more source

Pyrazinoic Acid Inhibits the Bifunctional Enzyme (Rv2783) in Mycobacterium tuberculosis by Competing with tmRNA

Pathogens, 2019
Pyrazinamide (PZA) is a key drug for tuberculosis treatment. The active form of PZA, pyrazinoic acid (POA), appears to inhibit multiple targets in M. tuberculosis. Recently, the bifunctional enzyme Rv2783 was reported as a new target of POA. However, the
Lei He, Peng Cui, Wanliang Shi, Qiong Li, Wenhong Zhang, Min Li, Ying Zhang   +6 more
doaj   +1 more source

Computational evolution of an RNA-binding protein towards enhanced oxidized-RNA binding

Computational and Structural Biotechnology Journal, 2020
The oxidation of RNA has been implicated in the development of many diseases. Among the four ribonucleotides, guanosine is the most susceptible to oxidation, resulting in the formation of 8-oxo-7,8-dihydroguanosine (8-oxoG). Despite the limited knowledge
Juan C. Gonzalez-Rivera, Asuka A. Orr, Sean M. Engels, Joseph M. Jakubowski, Mark W. Sherman, Katherine N. O'Connor, Tomas Matteson, Brendan C. Woodcock, Lydia M. Contreras, Phanourios Tamamis   +9 more
doaj   +1 more source

Polyribosome-Dependent Clustering of Membrane-Anchored RNA Degradosomes To Form Sites of mRNA Degradation in Escherichia coli

mBio, 2021
Here, we show that RNase E, RhlB, and PNPase act together as components of the multienzyme RNA degradosome in polyribosome-dependent clustering to form puncta on the inner cytoplasmic membrane.
Lina Hamouche, Leonora Poljak, Agamemnon J. Carpousis   +2 more
doaj   +1 more source