Results 61 to 70 of about 4,003 (181)

No Correlation Between Interferon Signaling and Cytosolic Mitochondrial DNA/RNA Leakage in Cultured Skin Fibroblasts of Patients With Mitochondrial Diseases

European Journal of Immunology, Volume 56, Issue 4, April 2026.
Investigation of IFN signaling in cultured skin fibroblasts from patients with genetically confirmed mitochondrial diseases of diverse origins revealed that altered IFN signaling is an inconsistent feature of these disorders. Cytosolic accumulation of mtDNA and mtRNA was variably detected and showed little correlation with ISG scores.
Manon Marchais, Alessandra Pennisi, Alexandre Pierga, Alice Lepelley, Nicolas Cagnard, Christine Bole, Patrick Nitschke, Mohamed Hamici, Frédéric Rieux‐Laucat, Manuel Schiff, Arnold Munnich, Agnès Rötig   +11 more
wiley   +1 more source

Human FASTK preferentially binds single‐stranded and G‐rich RNA

The FEBS Journal, Volume 293, Issue 3, Page 729-748, February 2026.
Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Daria M. Dawidziak, Dawid A. Dzadz, Mikołaj I. Kuska, Madhuri Kanavalli, Maria M. Klimecka, Matthew Merski, Katarzyna J. Bandyra, Maria W. Górna   +7 more
wiley   +1 more source

PNPase IN C. ELEGANS: MUTAGENIC ANALYSIS TO COMPLEMENT KNOCKDOWN STUDIES [PDF]

, 2017
PNPase is a gene implicated as a potential target for cancer therapy; human mutations also present with deafness, myopathies, and neuropathies. In this study, C. elegans was used to investigate the effect of knocking out PNPase in a whole animal.
Seibert, Danielle K.
core   +1 more source

Selective RNA Processing and Stabilization are Multi‐Layer and Stoichiometric Regulators of Gene Expression in Escherichia coli

Advanced Science, 2023
Selective RNA processing and stabilization (SRPS) facilitates the differential expression of multiple genes in polycistronic operons. However, how the coordinated actions of SRPS‐related enzymes affect stoichiometric regulation remains unclear.
Daixi Liu, Haibo Lv, Yafei Wang, Jinyu Chen, Dexin Li, Ranran Huang   +5 more
doaj   +1 more source

Biocatalytic Construction of a Chemical Exchange Saturation Transfer Magnetic Resonance Imaging Nucleoside Probe: Synthesis and Evaluation of 5‐Methyl‐5,6‐Dihydrothymidine

Advanced Synthesis &Catalysis, Volume 368, Issue 1, January 2026.
Al‐Hilfi et al. present a biocatalytic strategy for synthesizing 5‐methyl‐5,6‐dihydrothymidine (5‐MDHT), a sensitive MRI contrast agent. The study demonstrates that recombinant enzyme catalysis offers an efficient, sustainable, and eco‐friendly alternative to traditional chemical synthesis for producing clinically relevant imaging probes.
Aimen Al‐Hilfi, E. Alejandro Castellanos Franco, Connor J. Grady, Zinia Mohanta, Michael T. McMahon, Milana Bazayeva, Zhen Li, Kenneth M. Merz Jr., Assaf A. Gilad   +8 more
wiley   +1 more source

Domains and structure of PNPase.

, 2018
(A) Domain organization of PNPase, using the C. crescentus PNPase crystal structure as a reference [44]. The complete PNPase trimer viewed from the (B) top and (C) side, with domains colored the same as in (A). (D) A cut-away view of the RNA-bound trimer
Todd A. Cameron (146712), Lisa M. Matz (5847950), Nicholas R. De Lay (5847953)   +2 more
core   +1 more source

Graphene‐Based Liquid Cell Designs for In Situ Liquid‐Phase Transmission Electron Microscopy: Recent Developments and Perspectives

Small Science, Volume 5, Issue 12, December 2025.
This review highlights the advances in graphene liquid cell designs for in situ liquid‐phase transmission electron microscopy, spanning veil‐type, well‐type, liquid‐flowing‐type, and mixing‐type configurations. These developments collectively enable real‐time visualization of diverse reactions in liquid environments at atomic resolution, thereby ...
Hyeonjong Ma, Hyeongseung Kim, Jiwoong Yang   +2 more
wiley   +1 more source

The ribonuclease E regulator RebA is essential for diazotrophic growth in the cyanobacterium Anabaena PCC 7120

mLife, Volume 4, Issue 5, Page 516-526, October 2025.
Abstract Ribonuclease E (RNase E) is central to bacterial RNA metabolism. In cyanobacteria, its activity is inhibited by RebA, a key mechanism for controlling cell morphology. Here, we demonstrate that rebA is essential for diazotrophic growth of Anabaena PCC 7120, a filamentous cyanobacterium capable of forming heterocysts—specialized nitrogen‐fixing ...
Sujuan Liu, Zhenyu Wang, Guiming Lin, Wenkai Li, Xiaoli Zeng, Ju‐Yuan Zhang, Cheng‐Cai Zhang   +6 more
wiley   +1 more source

Crystal structure of dimeric human PNPase reveals why disease-linked mutants suffer from low RNA import and degradation activities

Nucleic Acids Research, 2018
Human polynucleotide phosphorylase (PNPase) is an evolutionarily conserved 3′-to-5′ exoribonuclease principally located in mitochondria where it is responsible for RNA turnover and import.
B. Golzarroshan, Chia-Liang Lin, Chia-Lung Li, Wei-Zen Yang, Lee-Ya Chu, S. Agrawal, Hanna S. Yuan   +6 more
semanticscholar   +1 more source

Skeletal Muscle Mitochondria Contain Nuclear‐Encoded RNA Species Prior to and Following Adaptation to Exercise Training in Rats

The FASEB Journal, Volume 39, Issue 13, 15 July 2025.
Here, we report that mitochondria from rat skeletal muscle contain a select population of nuclear‐encoded protein‐coding and noncoding RNAs, including 3 microRNAs whose expression tended to be altered by exercise training. These findings suggest that mitochondria‐localized nuclear‐encoded RNAs may play a role in mediating the beneficial adaptive ...
Jessica L. Silver, Séverine Lamon, Stella Loke, Gisella Mazzarino, Larry Croft, Mark Ziemann, Megan Soria, Glenn D. Wadley, Adam J. Trewin   +8 more
wiley   +1 more source