Results 71 to 80 of about 4,319 (202)

Crystal structure of Escherichia coli PNPase: Central channel residues are involved in processive RNA degradation [PDF]

RNA, 2008
Bacterial polynucleotide phosphorylase (PNPase) plays a major role in mRNA turnover by the degradation of RNA from the 3′- to 5′-ends. Here, we determined the crystal structures of the wild-type and a C-terminal KH/S1 domain-truncated mutant (ΔKH/S1) of Escherichia coli PNPase at resolutions of 2.6 Å and 2.8 Å, respectively. The six RNase PH domains of
Zhonghao, Shi, Wei-Zen, Yang, Sue, Lin-Chao, Kin-Fu, Chak, Hanna S, Yuan   +4 more
openaire   +2 more sources

Cytoplasmic RNA decay pathways - enzymes and mechanisms [PDF]

, 2016
RNA decay plays a crucial role in post-transcriptional regulation of gene expression. Work conducted over the last decades has defined the major mRNA decay pathways, as well as enzymes and their cofactors responsible for these processes. In contrast, our
Dziembowski, Andrzej, Tomecki, Rafał, Łabno, Anna   +2 more
core   +1 more source

Skeletal Muscle Mitochondria Contain Nuclear‐Encoded RNA Species Prior to and Following Adaptation to Exercise Training in Rats

The FASEB Journal, Volume 39, Issue 13, 15 July 2025.
Here, we report that mitochondria from rat skeletal muscle contain a select population of nuclear‐encoded protein‐coding and noncoding RNAs, including 3 microRNAs whose expression tended to be altered by exercise training. These findings suggest that mitochondria‐localized nuclear‐encoded RNAs may play a role in mediating the beneficial adaptive ...
Jessica L. Silver, Séverine Lamon, Stella Loke, Gisella Mazzarino, Larry Croft, Mark Ziemann, Megan Soria, Glenn D. Wadley, Adam J. Trewin   +8 more
wiley   +1 more source

An RNA-seq based comparative approach reveals the transcriptome-wide interplay between 3′-to-5′ exoRNases and RNase Y

Nature Communications, 2020
Bacterial RNA degradation is typically initiated by endoribonucleases and followed by exoribonucleases. Here the authors report the targetome of endoRNase Y in Streptococcus pyogenes, revealing the interplay between RNase Y and 3′-to-5′ exoribonuclease ...
Laura Broglia, Anne-Laure Lécrivain, Thibaud T. Renault, Karin Hahnke, Rina Ahmed-Begrich, Anaïs Le Rhun, Emmanuelle Charpentier   +6 more
doaj   +1 more source

Systematic analysis of plant mitochondrial and chloroplast small RNAs suggests organelle-specific mRNA stabilization mechanisms [PDF]

, 2016
Land plant organellar genomes encode a small number of genes, many of which are essential for respiration and photosynthesis. Organellar gene expression is characterized by a multitude of RNA processing events that lead to stable, translatable ...
Gusewski, Sandra, Ruwe, Hannes, Schmitz-Linneweber, Christian, Wang, Gongwei   +3 more
core   +1 more source

Phase Separation Regulates Metabolism, Mitochondria, and Diseases

MedComm, Volume 6, Issue 7, July 2025.
Mitochondrion‐related liquid–liquid phase separation. (1) Phase separation mediates the self‐assembly of mitochondrial nucleoids (mt‐nucleoids). (2) Phase segregation mediates the formation of mitochondrial RNA granules (MRGs). (3) Phase separation mediates the formation of mitochondrial degradosomes.
Chuan Gao, Peng Ding, Changqing Zhang, Junjie Gao   +3 more
wiley   +1 more source

Rapid Degradation of Hfq-Free RyhB inYersinia pestisby PNPase Independent of Putative Ribonucleolytic Complexes [PDF]

BioMed Research International, 2014
The RNA chaperone Hfq in bacteria stabilizes sRNAs by protecting them from the attack of ribonucleases. Upon release from Hfq, sRNAs are preferably degraded by PNPase. PNPase usually forms multienzyme ribonucleolytic complexes with endoribonuclease E and/or RNA helicase RhlB to facilitate the degradation of the structured RNA.
Zhongliang Deng, Zizhong Liu, Yujing Bi, Xiaoyi Wang, Dongsheng Zhou, Ruifu Yang, Yanping Han   +6 more
openaire   +2 more sources

Target recognition by RNase E RNA-binding domain AR2 drives sRNA decay in the absence of PNPase

Proceedings of the National Academy of Sciences, 2022
The C-terminal domain (CTD) of the major endoribonuclease RNase E not only serves as a scaffold for the central RNA decay machinery in gram-negative bacteria but also mediates coupled degradation of small regulatory RNAs (sRNAs) and their cognate target transcripts following RNA chaperone Hfq–facilitated sRNA–mRNA base pairing. Despite the
Dhriti Sinha, Nicholas R. De Lay
openaire   +2 more sources

Crystal structure of the S. solfataricus archaeal exosome reveals conformational flexibility in the RNA-binding ring. [PDF]

PLoS ONE, 2010
The exosome complex is an essential RNA 3'-end processing and degradation machinery. In archaeal organisms, the exosome consists of a catalytic ring and an RNA-binding ring, both of which were previously reported to assume three-fold symmetry.Here we ...
Changrui Lu, Fang Ding, Ailong Ke
doaj   +1 more source

Quick change: post-transcriptional regulation in Pseudomonas [PDF]

, 2017
Pseudomonas species have evolved dynamic and intricate regulatory networks to fine-tune gene expression, with complex regulation occurring at every stage in the processing of genetic information.
Afonyushkin, Agalarov, Andrade, Arce-Rodriguez, Baker, Barquist, Berghoff, Bhagirath, Boudry, Brencic, Brencic, Broder, Bubunenko, Cech, Chambers, Chambonnier, Chatterjee, Coggan, Desnoyers, Duss, Fang, Fernandez, Fields, Gao, Goodman, Goodman, Heurlier, Holmqvist, Holmqvist, Hsu, Ingolia, Irie, Jacob G. Malone, Jean-Pierre, Jorgensen, Jorgensen, Kang, Kong, Lapouge, Lin, Link, Little, Lu, Lucia Grenga, Maki, Melamed, Meyer, Mikulecky, Miller, Moll, Moller, Morita, Moscoso, Mulcahy, Nesterchuk, Petrova, Reimmann, Richard H. Little, Schubert, Schulmeyer, Sittka A, Sobrero, Sonnleitner, Sonnleitner, Sonnleitner E, Takeuchi, Thomason, Unwin, Valentin-Hansen, Van Assche, Ventre, Vogel, Wang, Wang, Wei, Zhang, Zhang   +76 more
core   +1 more source