Results 91 to 100 of about 4,003 (181)

A type III-A CRISPR-Cas system employs degradosome nucleases to ensure robust immunity

eLife, 2019
CRISPR-Cas systems provide sequence-specific immunity against phages and mobile genetic elements using CRISPR-associated nucleases guided by short CRISPR RNAs (crRNAs).
Lucy Chou-Zheng, Asma Hatoum-Aslan
doaj   +1 more source

Identification of Polynucleotide Phosphorylase (PNPase) in Escherichia coli Involved in Persister Formation

bioRxiv, 2018
Despite the identification of many genes and pathways involved in the persistence phenomenon of bacteria, the mechanisms of persistence are not well understood.
Nan Wu, Yumeng Zhang, Shanshan Zhang, Youhua Yuan, Shuang Liu, P. Cui, Wen Zhang, Ying Zhang   +7 more
semanticscholar   +1 more source

Strategies for Detecting Aminoacylation and Aminoacyl‐tRNA Editing in vitro and in Cells

Israel Journal of Chemistry, Volume 64, Issue 8-9, September 2024.
Abstract Aminoacyl‐tRNA synthetases (aaRSs) maintain translational fidelity by ensuring the formation of correct aminoacyl‐tRNA pairs. Numerous point mutations in human aaRSs have been linked to disease phenotypes. Structural studies of aaRSs from human pathogens encoding unique domains support these enzymes as potential candidates for therapeutics ...
Rylan R. Watkins, Arundhati Kavoor, Karin Musier‐Forsyth   +2 more
wiley   +1 more source

PNPase Independent of Putative Ribonucleolytic Complexes

, 2020
The RNA chaperone Hfq in bacteria stabilizes sRNAs by protecting them from the attack of ribonucleases. Upon release from Hfq, sRNAs are preferably degraded by PNPase. PNPase usually forms multienzyme ribonucleolytic complexes with endoribonuclease E and/
Yujing Bi, Zizhong Liu, Yanping Han, Ruifu Yang, Xiaoyi Wang, Dongsheng Zhou, Zhongliang Deng   +6 more
core  

Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli

BMC Genomics, 2015
The RNA steady-state levels in the cell are a balance between synthesis and degradation rates. Although transcription is important, RNA processing and turnover are also key factors in the regulation of gene expression. In Escherichia coli there are three
V. Pobre, C. Arraiano
semanticscholar   +1 more source

Single‐Molecule Electrical Profiling of Peptides and Proteins

Advanced Science, Volume 11, Issue 28, July 24, 2024.
This work presents a timely overview of the prominent research progress in peptide and protein‐related studies through single‐molecule electrical techniques, including single‐molecule junction, single‐molecule field‐effect transistor (FET), and nanopore.
Hongyu Ju, Li Cheng, Mengmeng Li, Kunrong Mei, Suhang He, Chuancheng Jia, Xuefeng Guo   +6 more
wiley   +1 more source

Roles and partners of bacterial and human PNPase.

, 2018
Roles and partners of bacterial and human PNPase.
Todd A. Cameron (146712), Lisa M. Matz (5847950), Nicholas R. De Lay (5847953)   +2 more
core   +1 more source

Continuous enzyme-coupled assay of phosphate- or pyrophosphate-releasing enzymes

BioTechniques, 2012
A coupled enzyme assay able to monitor the kinetics of reactions catalyzed by phosphate- or pyrophosphate-releasing enzymes is presented here.
Antonio S. Guillén Suárez, Alessandra Stefan, Silvia Lemma, Emanuele Conte, Alejandro Hochkoeppler   +4 more
doaj   +1 more source

A conserved loop in polynucleotide phosphorylase (PNPase) essential for both RNA and ADP/phosphate binding

, 2014
Polynucleotide phosphorylase (PNPase) reversibly catalyzes RNA phosphorolysis and polymerization of nucleoside diphosphates. Its homotrimeric structure forms a central channel where RNA is accommodated.
F. Briani, T. Carzaniga, E. Mazzantini, M. E. Regonesi, G. Dehò, C. Greco, P. Tortora, M. Nardini, L. De Gioia   +8 more
core   +1 more source

The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome

Bioscience Reports, 2014
Multiprotein complexes that carry out RNA degradation and processing functions are found in cells from all domains of life. In Escherichia coli, the RNA degradosome, a four-protein complex, is required for normal RNA degradation and processing.
Feng Lu, Aziz Taghbalout
doaj   +1 more source