Results 201 to 208 of about 3,302 (208)

The RNA degradosome: life in the fast lane of adaptive molecular evolution

Trends in Biochemical Sciences, 2006
In Escherichia coli, the multi-enzyme RNA degradosome contributes to the global, posttranscriptional regulation of gene expression. The degradosome components are recognized through natively unstructured "microdomains" comprising as few as 15-40 amino acids.
Agamemnon J. Carpousis, Vidya Chandran, Ben F. Luisi, Maria J. Marcaida, Mark A. DePristo   +4 more
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Preparation of Escherichia coli Rne Protein and Reconstitution of RNA Degradosome

2001
Publisher Summary This chapter describes methods for the purification of Rne, the key scaffold in the assembly process, and for the reconstitution of active degradosomes from individually purified enzymes. The endoribonuclease RNase E was initially described as an activity required for the penultimate step in the maturation of 5S rRNA, the processing
Glen A. Coburn, Douglas J. Briant, George A. Mackie, Xin Miao, Annie Prud’Homme-Genereux   +4 more
openaire   +3 more sources

Composition and adpatation of the E. coli RNA degradosome

2004
Although transcription and translation are recognized mechanisms for regulating gene expression, control over RNA stability can also accomplish this task. In E. coli, bulk mRNA degradation is largely carried out by a complex of enzymes called the degradosome. It is composed of the endonuclease RNase E, the exonuclease PNPase, the helicase RhIB, and the
openaire   +2 more sources

Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome

2009
The mitochondrial degradosome (mtEXO) is the main enzymatic complex in RNA degradation, processing, and surveillance in Saccharomyces cerevisiae mitochondria. It consists of two nuclear-encoded subunits: the ATP-dependent RNA helicase Suv3p and the 3' to 5' exoribonuclease Dss1p. The two subunits depend on each other for their activity; the complex can
Michal Malecki, Pawel Golik, Piotr P. Stepien   +2 more
openaire   +3 more sources

Structural Studies of the Yeast Mitochondrial Degradosome

2005
The yeast mitochondrial degradosome/exosome (mtExo) is responsible for most RNA turnover in mitochondria and has been proposed to form a central part of a mitochondrial RNA surveillance system responsible for degradation of aberrant and unprocessed RNA ([1], [2]).
Feddersen, Ane, Jonstrup, Anette Thyssen, Brodersen, Ditlev Egeskov   +2 more
openaire   +2 more sources

Dissection of the Network of Interactions That Links RNA Processing with Glycolysis in the Bacillus subtilis Degradosome

Journal of Molecular Biology, 2012
The RNA degradosome is a multiprotein macromolecular complex that is involved in the degradation of messenger RNA in bacteria. The composition of this complex has been found to display a high degree of evolutionary divergence, which may reflect the adaptation of species to different environments.
Newman JA, Hewitt L, Rodrigues C, Solovyova AS, Harwood CR, Lewis RJ   +5 more
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Genetic and Biochemical Approaches for Analysis of Mitochondrial Degradosome from Saccharomyces cerevisiae

2001
Publisher Summary Regulation of RNA turnover is one of the major mechanisms of controlling gene expression in yeast mitochondria. RNA degradation is carried out by the yeast mitochondrial degradosome (also known as mtEXO); a multiprotein enzyme complex showing NTP-dependent 3′ → 5′-exoribonuclease activity in vitro.
Andrzej Dziembowski, Piotr P. Stepien
openaire   +3 more sources

Degradosome

2008
openaire   +1 more source