Results 11 to 20 of about 873 (157)

Vitamin B12 as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter [PDF]

MicrobiologyOpen
Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and ...
Elizabeth Phillips, Katherine Picott, Steffen Kümmel, Olivia Bulka, Elizabeth Edwards, Po‐Hsiang Wang, Matthias Gehre, Ivonne Nijenhuis, Barbara S. Lollar   +8 more
doaj   +5 more sources

From Energy Deprivation Stress to Catalytic Adaptation of Reductive Dehalogenase TmrA for 1,1‐DCA Dechlorination: An Experimental and in Silico Study [PDF]

Microbial Biotechnology
Organohalide‐respiring bacteria capable of metabolizing multiple organohalogens represent valuable tools for bioremediation and offer intriguing evolutionary potential for adaptation to non‐native substrates.
Yi Ren, Evan Landers, Matthew Lee, Mike Manefield   +3 more
doaj   +3 more sources

Comparative proteogenomics reveals ecological and evolutionary insights into the organohalide-respiring Dehalobacter restrictus strain T [PDF]

Applied and Environmental Microbiology
1,1,1-Trichloroethane (1,1,1-TCA) and chloroform (CF) are persistent groundwater contaminants because of their widespread industrial use as organic solvents and improper disposal in the past.
Xiaocui Li, Xiuying Li, Huijuan Jin, Jingjing Wang, Lian Yu, Jun Yan, Yi Yang   +6 more
doaj   +4 more sources

Complete genome sequence of Dehalobacter restrictus PER-K23T [PDF]

Standards in Genomic Sciences, 2013
Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or ...
David Bruce, Cliff Han, Ioanna Pagani, Victor Markowitz, Natalia Ivanova, Hauke Smidt, Holliger, Christof, Pagani, Ioanna, Christof Holliger, Kyrpides, Nikos, Amy Chen, Woyke, T., Chen, A., Goodwin, Lynne, Julien Maillard, Hazuki Teshima, Wei, C.L., Woyke, Tanja, Teshima, H., James Han, Tanja Woyke, Maillard, Julien, Smidt, Hauke, Ivanova, N., Pati, A., Chia-Lin Wei, Huntemann, Marcel, Roxanne Tapia, Maillard, J., Detter, J.C., Kyrpides, N., Sam Pitluck, Markowitz, V., Szeto, E., Bruce, D.C., Bruce, David, Smidt, H., Kruse, T.K., Nolan, M., Goodwin, L.A., Ivanova, Natalia, Han, Cliff, Huntemann, M., Teshima, Hazuki, Nikos Kyrpides, Detter, Chris, Matt Nolan, Pagani, I., Szeto, Ernest, Wei, Chia-Lin, Ernest Szeto, Markowitz, Victor, Amrita Pati, Han, C., Holliger, C., Han, James, Nolan, Matt, Pitluck, S., Chen, Amy, Han, J., Pitluck, Sam, Marcel Huntemann, Tapia, Roxanne, Lynne Goodwin, Tapia, R., Pati, Amrita, Kruse, Thomas, Thomas Kruse, Chris Detter, Szeto E, Bruce D, Woyke T, Teshima H, Goodwin L, Ivanova N, Huntemann M, Tapia R, Pitluck S, Markowitz V, Pagani I, Holliger C, Maillard J, Chen A, Detter C, Wei CL, Nolan M, Kruse T, Pati A, Kyrpides N, Han J, Smidt H, Han C   +91 more
core   +7 more sources

The Genus Dehalobacter

, 2016
The genus Dehalobacter embraces bacterial populations that seem to exclusively degrade organohalides. All isolates in pure culture and highly enriched strains are obligate organohalide-respiring bacteria that use hydrogen as energy and electron source ...
Holliger, Christof, Maillard, Julien, Julien Maillard, Christof Holliger   +3 more
core   +2 more sources

Pangenomic insights into <i>Dehalobacter</i> evolution and acquisition of functional genes for bioremediation. [PDF]

Microb Genom
Dehalobacter is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a Dehalobacter population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the mec cassette, just downstream of its active ...
Bulka O, Mahadevan R, Edwards EA.
europepmc   +3 more sources

Genome sequence of Dehalobacter sp. strain TeCB1, able to respire chlorinated benzenes [PDF]

Genome Announcements, 2017
Dehalobacter sp. strain TeCB1 was isolated from groundwater contaminated with a mixture of organohalides and is able to respire 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene.
Ertan, H ; https://orcid.org/, Ertan, Haluk, Lee, M, Ricardo Alfán-Guzmán, Manefield, Mike, Manefield, M, Manefield, M ; https://orcid.org/, Ertan, H, Matthew Lee, Alfán-Guzmán, R, Alfan Guzman, Ricardo, Haluk Ertan, Lee, M ; https://orcid.org/, Mike Manefield, Lee, Matthew, Alfan-Guzman, Ricardo   +15 more
core   +5 more sources

Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases [PDF]

Applied and Environmental Microbiology
Reductive dehalogenases (RDases) are essential in the anaerobic degradation of various organohalide contaminants. This family of enzymes has broad sequence diversity, but high structural conservation. There have been few studies assessing how RDase amino
Katherine J. Picott, Connor M. Bowers, Elizabeth A. Edwards   +2 more
doaj   +2 more sources

A bacterial chloroform reductive dehalogenase: purification and biochemical characterization

Microbial Biotechnology, 2017
Summary We report herein the purification of a chloroform (CF)‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared
Bat‐Erdene Jugder, Susanne Bohl, Helene Lebhar, Robert D. Healey, Mike Manefield, Christopher P. Marquis, Matthew Lee   +6 more
doaj   +2 more sources

Developing an E. coli heterologous expression system for characterizing a marine debrominase from Roseobacter sp. [PDF]

Applied and Environmental Microbiology
Reductive dehalogenases (RDases) are a family of enzymes that employ a cobamide cofactor and two iron-sulfur clusters to selectively cleave carbon-halogen bonds.
Xiaofang Li, Sen Yang, Yuping Liu, Hua Huang, Xinshuai Zhang, Yin Zhong, Lorenz Adrian, Ping’an Peng   +7 more
doaj   +2 more sources