Vitamin B12 as a source of variability in isotope effects for chloroform biotransformation by Dehalobacter [PDF]
Carbon and chlorine isotope effects for biotransformation of chloroform by different microbes show significant variability. Reductive dehalogenases (RDase) enzymes contain different cobamides, affecting substrate preferences, growth yields, and ...
Elizabeth Phillips +8 more
doaj +5 more sources
Accelerating bioelectrodechlorination via data-driven inverse design [PDF]
Microbial electrorespiration harnesses bacteria to drive reductive dechlorination, offering a sustainable method to remediate environments contaminated with persistent chlorinated organic pollutants (COPs).
Zhiling Li +4 more
doaj +2 more sources
A bacterial chloroform reductive dehalogenase: purification and biochemical characterization
Summary We report herein the purification of a chloroform (CF)‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared
Bat‐Erdene Jugder +6 more
doaj +2 more sources
Whole metagenome sequencing and 16S rRNA gene amplicon analyses reveal the complex microbiome responsible for the success of enhanced in-situ reductive dechlorination (ERD) of a tetrachloroethene-contaminated Superfund site. [PDF]
The North Railroad Avenue Plume (NRAP) Superfund site in New Mexico, USA exemplifies successful chlorinated solvent bioremediation. NRAP was the result of leakage from a dry-cleaning that operated for 37 years.
Rebecca A Reiss +3 more
doaj +2 more sources
Global prevalence of organohalide-respiring bacteria dechlorinating polychlorinated biphenyls in sewage sludge [PDF]
Background Massive amounts of sewage sludge are generated during biological sewage treatment and are commonly subjected to anaerobic digestion, land application, and landfill disposal.
Guofang Xu +4 more
doaj +2 more sources
Pangenomic insights into <i>Dehalobacter</i> evolution and acquisition of functional genes for bioremediation. [PDF]
Dehalobacter is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a Dehalobacter population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the mec cassette, just downstream of its active ...
Bulka O, Mahadevan R, Edwards EA.
europepmc +3 more sources
Anaerobic Biodegradation of Chloroform and Dichloromethane with a Dehalobacter Enrichment Culture. [PDF]
Chloroform (CF) and dichloromethane (DCM) are among the more commonly identified chlorinated aliphatic compounds found in contaminated soil and groundwater. One way to address this problem is to add microbes to the subsurface that can biodegrade these compounds.
Wang H +4 more
europepmc +4 more sources
Semi-automatic in silico gap closure enabled de novo assembly of two Dehalobacter genomes from metagenomic data. [PDF]
Typically, the assembly and closure of a complete bacterial genome requires substantial additional effort spent in a wet lab for gap resolution and genome polishing.
Shuiquan Tang +2 more
doaj +1 more source
Complete genome sequence of Dehalobacter restrictus PER-K23T [PDF]
Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene.
Kruse, T.K. +22 more
openaire +5 more sources
Burning question: Rethinking organohalide degradation strategy for bioremediation applications. [PDF]
This review specifically focuses on the novel solution of organohalide bioremediation, proposing redox‐potential‐mediated hybrid bioprocesses tailored to the complexities of organohalide pollution. Abstract Organohalides are widespread pollutants that pose significant environmental hazards due to their high degree of halogenation and elevated redox ...
Lu Q, Liang Q, Wang S.
europepmc +2 more sources

