Heterologous Expression of Active Dehalobacter Respiratory Reductive Dehalogenases in Escherichia coli. [PDF]
Appl Environ Microbiol, 2022 Understanding microbial reductive dehalogenation is important to refine the global halogen cycle and to improve bioremediation of halogenated contaminants; however, studies of the family of enzymes responsible are limited.
Picott KJ, Flick R, Edwards EA.
europepmc +4 more sources
Two distinct <i>Dehalobacter</i> metagenome-assembled genomes from anaerobic chloroform and dichloromethane degrading consortia. [PDF]
Microbiol Resour AnnouncHere we present two metagenomes and two Dehalobacter metagenome-assembled genomes from subcultures of an anaerobic chloroform and dichloromethane degrading microbial community used for bioremediation. Our objective was to assemble and curate the genome(s)
Bulka O, Edwards EA.
europepmc +4 more sources
Environmental Science & Technology, 2023 Chloroform (CF) and dichloromethane (DCM) contaminate groundwater sites around the world, which can be remediated through bioaugmentation. Although several strains of Dehalobacter restrictus can reduce CF to DCM, and multiple Peptococcaceae can ferment ...
Olivia Bulka +4 more
semanticscholar +3 more sources
A gap-filling algorithm for prediction of metabolic interactions in microbial communities. [PDF]
PLoS Computational Biology, 2021 The study of microbial communities and their interactions has attracted the interest of the scientific community, because of their potential for applications in biotechnology, ecology and medicine.
Dafni Giannari +2 more
doaj +3 more sources
Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases [PDF]
Applied and Environmental MicrobiologyReductive dehalogenases (RDases) are essential in the anaerobic degradation of various organohalide contaminants. This family of enzymes has broad sequence diversity, but high structural conservation. There have been few studies assessing how RDase amino
Katherine J. Picott +2 more
doaj +2 more sources
Diverse reductive dehalogenases are associated with Clostridiales-enriched microcosms dechlorinating 1,2-dichloroethane [PDF]
BioMed Research International, Volume 2015, Issue 1, 2015., 2015 The achievement of successful biostimulation of active microbiomes for the cleanup of a polluted site is strictly dependent on the knowledge of the key microorganisms equipped with the relevant catabolic genes responsible for the degradation process.
Balloi, Annalisa +8 more
core +6 more sources
Complete genome sequence of Dehalobacter restrictus PER-K23T [PDF]
Standards in Genomic Sciences, 2013 Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or ...
T. Kruse +22 more
semanticscholar +5 more sources
Developing an E. coli heterologous expression system for characterizing a marine debrominase from Roseobacter sp. [PDF]
Applied and Environmental MicrobiologyReductive dehalogenases (RDases) are a family of enzymes that employ a cobamide cofactor and two iron-sulfur clusters to selectively cleave carbon-halogen bonds.
Xiaofang Li +7 more
doaj +2 more sources
Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater. [PDF]
Journal of Microbiological Methods, 2016 Nucleic acid amplification of biomarkers is increasingly used to monitor microbial activity and assess remedial performance in contaminated aquifers. Previous studies described the use of filtration, elution, and direct isothermal amplification (i.e.
Robert D. Stedtfeld +6 more
semanticscholar +3 more sources
Bibliometrics and Visualization Analysis of Three Obligate Organohalide Respiring Bacteria Genera: A Systematic Review [PDF]
MicroorganismsOrganohalide-respiring bacteria (OHRB) facilitate the reductive dehalogenation of toxic halogenated compounds in the environment, which supports their growth and proliferation. Research conducted on OHRB has achieved notable advancements.
Lisi Jiang +6 more
doaj +2 more sources