Whole metagenome sequencing and 16S rRNA gene amplicon analyses reveal the complex microbiome responsible for the success of enhanced in-situ reductive dechlorination (ERD) of a tetrachloroethene-contaminated Superfund site. [PDF]
The North Railroad Avenue Plume (NRAP) Superfund site in New Mexico, USA exemplifies successful chlorinated solvent bioremediation. NRAP was the result of leakage from a dry-cleaning that operated for 37 years.
Rebecca A Reiss +3 more
doaj +2 more sources
Redox chemistry of cobalamin and iron‐sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus [PDF]
Respiration of Dehalobacter restrictus is based on reductive dechlorination of tetrachloroethene. The terminal component of the respiratory chain is the membrane‐bound tetrachloroethene reductase. The metal prosthetic groups of the purified enzyme have been studied by optical and EPR spectroscopy. The 60‐kDa monomer contains one cobalamin with E m(Co1+/
Schumacher, W. +3 more
openaire +3 more sources
Characterization of the Corrinoid Iron-Sulfur ProteinTetrachloroethene Reductive Dehalogenase of Dehalobacterrestrictus [PDF]
ABSTRACT The membrane-bound tetrachloroethene reductive dehalogenase (PCE-RDase) (PceA; EC 1.97.1.8), the terminal component of the respiratory chain of Dehalobacter restrictus , was purified 25-fold to apparent electrophoretic homogeneity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed
Maillard Julien +5 more
openaire +5 more sources
The role of bacteria and zerovalent iron (Fe(0)) in the degradation of chlorinated solvents in subsurface environments is of interest to researchers and remediation practitioners alike. Fe(0) used in reactive iron barriers for groundwater remediation positively interacted with enrichment cultures containing Dehalobacter strains in the transformation of
Matthew, Lee +6 more
openaire +3 more sources
Global prevalence of organohalide-respiring bacteria dechlorinating polychlorinated biphenyls in sewage sludge [PDF]
Background Massive amounts of sewage sludge are generated during biological sewage treatment and are commonly subjected to anaerobic digestion, land application, and landfill disposal.
Guofang Xu +4 more
doaj +2 more sources
Summary Dehalobacter sp. strain UNSWDHB can dechlorinate up to 4 mM trichloromethane at a rate of 0.1 mM per day to dichloromethane and 1,1,2‐trichloroethane (1 mM, 0.1 mM per day) with the unprecedented product profile of 1,2‐dichloroethane and vinyl chloride.
Wong, YK +4 more
openaire +4 more sources
Pangenomic insights into Dehalobacter evolution and acquisition of functional genes for bioremediation. [PDF]
Dehalobacter is a genus of organohalide-respiring bacteria that is recognized for its fastidious growth using reductive dehalogenases (RDases). In the SC05 culture, however, a Dehalobacter population also mineralizes dichloromethane (DCM) produced by chloroform dechlorination using the mec cassette, just downstream of its active ...
Bulka O, Mahadevan R, Edwards EA.
europepmc +3 more sources
Anaerobic Biodegradation of Chloroform and Dichloromethane with a Dehalobacter Enrichment Culture. [PDF]
Chloroform (CF) and dichloromethane (DCM) are among the more commonly identified chlorinated aliphatic compounds found in contaminated soil and groundwater. One way to address this problem is to add microbes to the subsurface that can biodegrade these compounds.
Wang H +4 more
europepmc +4 more sources
The development of rational and effective engineered bioremediation approaches for sites contaminated with chlorinated solvents requires a fundamental understanding of the factors limiting the in situ activity of dehalorespiring bacteria. Frequently, multiple dehalorespiring bacteria are present at contaminated sites, particularly when bioaugmentation ...
Yenjung, Lai, Jennifer G, Becker
openaire +3 more sources
Burning question: Rethinking organohalide degradation strategy for bioremediation applications. [PDF]
This review specifically focuses on the novel solution of organohalide bioremediation, proposing redox‐potential‐mediated hybrid bioprocesses tailored to the complexities of organohalide pollution. Abstract Organohalides are widespread pollutants that pose significant environmental hazards due to their high degree of halogenation and elevated redox ...
Lu Q, Liang Q, Wang S.
europepmc +2 more sources

