Whole metagenome sequencing and 16S rRNA gene amplicon analyses reveal the complex microbiome responsible for the success of enhanced in-situ reductive dechlorination (ERD) of a tetrachloroethene-contaminated Superfund site. [PDF]
PLoS ONEThe North Railroad Avenue Plume (NRAP) Superfund site in New Mexico, USA exemplifies successful chlorinated solvent bioremediation. NRAP was the result of leakage from a dry-cleaning that operated for 37 years.
Rebecca A Reiss +3 more
doaj +2 more sources
A bacterial chloroform reductive dehalogenase: purification and biochemical characterization
Microbial Biotechnology, 2017 Summary We report herein the purification of a chloroform (CF)‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared
Bat‐Erdene Jugder +6 more
doaj +2 more sources
Genome Sequence of Dehalobacter sp. Strain TeCB1, Able To Respire Chlorinated Benzenes [PDF]
Genome Announcements, 2017 Dehalobacter sp. strain TeCB1 was isolated from groundwater contaminated with a mixture of organohalides and is able to respire 1,2,4,5-tetrachlorobenzene and 1,2,4-trichlorobenzene. Here, we report its 3.13-Mb draft genome sequence.
Ricardo Alfán-Guzmán +3 more
semanticscholar +4 more sources
Isolation and Characterization of Dehalobacter sp. Strain TeCB1 Including Identification of TcbA: A Novel Tetra- and Trichlorobenzene Reductive Dehalogenase [PDF]
Frontiers in Microbiology, 2017 Dehalobacter sp. strain TeCB1 was isolated from groundwater near Sydney, Australia, that is polluted with a range of organochlorines. The isolated strain is able to grow by reductive dechlorination of 1,2,4,5-tetrachlorobenzene to 1,3- and 1,4 ...
Ricardo Alfán-Guzmán +3 more
semanticscholar +3 more sources
Heterologous expression of Dehalobacter spp. respiratory reductive dehalogenases in Escherichia coli [PDF]
bioRxiv, 2021 Reductive dehalogenases (RDases) are a family of redox enzymes that are required for anaerobic organohalide respiration, a microbial process that is useful in bioremediation.
Katherine J. Picott +2 more
semanticscholar +2 more sources
Global prevalence of organohalide-respiring bacteria dechlorinating polychlorinated biphenyls in sewage sludge [PDF]
MicrobiomeBackground Massive amounts of sewage sludge are generated during biological sewage treatment and are commonly subjected to anaerobic digestion, land application, and landfill disposal.
Guofang Xu +4 more
doaj +2 more sources
Complete genome sequence of Dehalobacter restrictus PER-K23T [PDF]
Standards in Genomic Sciences, 2013 Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or ...
T. Kruse +22 more
semanticscholar +4 more sources
Genome Sequence of Dehalobacter UNSWDHB, a Chloroform-Dechlorinating Bacterium [PDF]
Genome Announcements, 2013 The chloroform-respiring bacterium Dehalobacter UNSWDHB was isolated from subsurface soil contaminated with a mixture of organohalides, including chloroform. Here, we present its 3.2-Mb genome.
Nandan P. Deshpande +4 more
semanticscholar +3 more sources
Genome-scale metabolic network reconstruction of the chloroform-respiring Dehalobacter restrictus strain CF [PDF]
bioRxiv, 2018 Background Organohalide-respiring bacteria (OHRB) play an important role in the global halogen cycle and bioremediation of industrial sites contaminated with chlorinated organics. One notable OHRB is Dehalobacter restrictus strain CF, which is capable of
Kevin Correia, H. Ho, R. Mahadevan
semanticscholar +2 more sources
Environmental Science & Technology, 2019 Trichloromethane (TCM) is a frequently detected and persistent groundwater contaminant. Recent studies have reported that two closely related Dehalobacter strains (UNSWDHB and CF) transform TCM to dichloromethane, with inconsistent carbon isotope effects
Benjamin Heckel +6 more
semanticscholar +5 more sources