Over-Expression of Dehydroascorbate Reductase Improves Salt Tolerance, Environmental Adaptability and Productivity in Oryza sativa [PDF]
Antioxidants, 2022 Abiotic stress induces reactive oxygen species (ROS) generation in plants, and high ROS levels can cause partial or severe oxidative damage to cellular components that regulate the redox status.
Young-Saeng Kim +9 more
doaj +2 more sources
Arabidopsis thaliana dehydroascorbate reductase 2: Conformational flexibility during catalysis. [PDF]
Sci Rep, 2017 Abstract Dehydroascorbate reductase (DHAR) catalyzes the glutathione (GSH)-dependent reduction of dehydroascorbate and plays a direct role in regenerating ascorbic acid, an essential plant antioxidant vital for defense against oxidative stress.
Bodra N +8 more
europepmc +6 more sources
Dehydroascorbate and dehydroascorbate reductase are phantom indicators of oxidative stress in plants [PDF]
FEBS Letters, 1997 In many physiological studies dehydroascorbate (DHA) reductase is regarded as one of the chloroplast enzymes involved in the protection against oxidative stress. Here, evidence is presented that plant cells do not possess a specific DHA reductase. The DHA reductase activities measured in plant extracts are due to side reactions of proteins containing ...
Hartmut Follmann +2 more
exaly +4 more sources
Molecular cloning and characterization of a rice dehydroascorbate reductase [PDF]
FEBS Letters, 2000 Plant dehydroascorbate reductase (DHAR), which re‐reduces oxidized ascorbate to maintain an appropriate level of ascorbate, is very important, but no gene or cDNA for plant DHAR has been cloned yet. Here, we describe a cDNA for a rice glutathione‐dependent DHAR (designated DHAR1). A recombinant Dhar1p produced in Escherichia coli was functional.
Yasushi Maki +2 more
exaly +3 more sources
New role for thioredoxins in plants: Implication of TRXo1 in protein depersulfidation [PDF]
Redox BiologyPersulfidation, a posttranslational modification of cysteines to persulfides, is the best characterized molecular mechanism of H2S signaling. This study is focused on new functions for thioredoxins (TRXs) in plants beyond those of thiol disulfide (S–S ...
Sabrina De Brasi-Velasco +5 more
doaj +2 more sources
High Throughput Analyses of Ascorbate-turnover Enzyme Activities in Rice (Oryza sativa L.) Seedlings
Bio-Protocol, 2021 Ascorbate (Vitamin C) fulfills various functions in plant photosynthesis and abiotic stress tolerance. The four key enzymes involved in the ascorbate-turnover pathway are ascorbate peroxidase, ascorbate oxidase, monodehydroascorbate reductase, and ...
Lin Wu +4 more
doaj +1 more source
The presence of dehydroascorbate and dehydroascorbate reductase in plant tissues [PDF]
FEBS Letters, 1998 Ascorbate, an essential antioxidant in plant tissues, is oxidised to dehydroascorbate (DHA) via successive reversible single electron transfers with monodehydroascorbate (MDHA) as a free radical intermediate. Rapid regeneration of ascorbate from its oxidised forms is required to support antioxidant capacity.
Foyer, Christine H, Mullineaux, Philip M
openaire +2 more sources
Plants, 2021 Boron (B) performs physiological functions in higher plants as an essential micronutrient, but its protective role in salt stress is poorly understood. Soybean (Glycine max L.) is planted widely throughout the world, and salinity has adverse effects on ...
Hesham F. Alharby +4 more
doaj +1 more source
The pivotal function of dehydroascorbate reductase in glutathione homeostasis in plants [PDF]
Journal of Experimental Botany, 2020 Abstract Under natural conditions, plants are exposed to various abiotic and biotic stresses that trigger rapid changes in the production and removal of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2). The ascorbate-glutathione pathway has been recognized to be a key player in H2O2 metabolism, in which reduced glutathione (
Shengchun Li +3 more
openaire +2 more sources
Reduction of Dehydroascorbate to Ascorbate by the Selenoenzyme Thioredoxin Reductase [PDF]
Journal of Biological Chemistry, 1997 Recycling of ascorbate from its oxidized forms is essential to maintain stores of the vitamin in human cells. Whereas reduction of dehydroascorbate to ascorbate is thought to be largely GSH-dependent, we reconsidered the possibility that the selenium-dependent thioredoxin system might contribute to ascorbate regeneration.
J M, May +3 more
openaire +2 more sources