Assessment of the microbial communities associated with white syndrome and brown jelly syndrome in aquarium corals [PDF]
Bacterial and ciliate assemblages associated with aquarium corals displaying white syndrome (WS) and brown jelly syndrome (BJS) were investigated. Healthy (n = 10) and diseased corals (WS n = 18; BJS n = 3) were analysed for 16S rRNA gene bacterial ...
Bythell, John C. +3 more
core +1 more source
Hepatitis C virus 3'UTR regulates viral translation through direct interactions with the host translation machinery. [PDF]
The 3' untranslated region (3'UTR) of hepatitis C virus (HCV) messenger RNA stimulates viral translation by an undetermined mechanism. We identified a high affinity interaction, conserved among different HCV genotypes, between the HCV 3'UTR and the host ...
Bai, Yun +2 more
core +2 more sources
Mature red blood cells (RBCs) can capture extracellular DNA, with short fragments homologous to cfDNA. This uptake is mediated by apoptotic bodies, which induce RBC oxidative stress, deformation, and accelerated in vivo clearance. The rbcDNA abundance correlates with tumor burden and therapeutic response, highlighting its potential as a liquid biopsy ...
Zihang Zeng +20 more
wiley +1 more source
Temporal and spatial stability of ammonia-oxidizing archaea and bacteria in aquarium biofilters. [PDF]
Nitrifying biofilters are used in aquaria and aquaculture systems to prevent accumulation of ammonia by promoting rapid conversion to nitrate via nitrite.
Samik Bagchi +5 more
doaj +1 more source
Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward +2 more
core +1 more source
Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits
Xu et al. discovers that switching off auxin transporters in soybean, PIN3a and PIN3b, disrupts auxin flow; this triggers ARF‐STF3/4 signal cascade to activate the nitrate importer NPF2.13 and soil nitrogen acquisition. Multi‐year field trials show edited soybeans maintain yield with higher oil content, offering a potential genetic route to improve ...
Huifang Xu +16 more
wiley +1 more source
Purification of Cortactin Using Anion Exchange Chromatography [PDF]
Cortactin is an actin-binding protein that has been shown to be involved in cellular migration and metastases in cancer. Bacterially expressed and purified cortactin protein is often used in in vitro assays to examine cortactin’s role in promoting cell ...
Priester, Jacob
core +1 more source
Application of COMPOCHIP Microarray to Investigate the Bacterial Communities of Different Composts [PDF]
A microarray spotted with 369 different 16S rRNA gene probes specific to microorganisms involved in the degradation process of organic waste during composting was developed. The microarray was tested with pure cultures, and of the 30,258 individual probe-
A Alfreider +54 more
core +1 more source
We investigate by proteomics studies how strand‐symmetric and ‐asymmetric cytosine 5‐modifications in DNA are selectively recognized by the nuclear proteome. Using promoter probes with defined modification patterns, we identify tissue‐specific reader proteinsincluding MYC, MAX, and RFX5that discriminate 5‐hydroxymethylcytosine symmetry and sequence ...
Lena Engelhard +8 more
wiley +1 more source
Validation of Double Gradient Denaturing Gradient Gel Electrophoresis through Multigenic Retrospective Analysis [PDF]
Abstract Among established techniques for the identification of either known or new mutations, denaturing gradient gel electrophoresis (DGGE) is one of the most effective. However, conventional DGGE is affected by major drawbacks that limit its routine application: the different denaturant gradient ranges and migration times required for
CREMONESI L. +9 more
openaire +3 more sources

