The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa [PDF]
BMC Veterinary Research, 2022 Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls.
Maria Eberhardt +5 more
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Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation [PDF]
Nanomaterials, 2016 Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors.
Monika Mortimer +3 more
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Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures [PDF]
MethodsX, 2023 Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay +4 more
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Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90% [PDF]
MethodsX, 2021 Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa +8 more
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Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation [PDF]
BMC Research Notes, 2023 Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza +5 more
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Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]
Reproductive Medicine and BiologyPurpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano +15 more
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Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia [PDF]
Life, 2021 In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler +5 more
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Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]
International Journal of Fertility and Sterility, 2020 Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini +6 more
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Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation [PDF]
The World Journal of Men's Health, 2021 Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated.
Gabriela Hernández-Silva +7 more
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Fractionating microplastics by density gradient centrifugation: a novel approach using LuerLock syringes in a low-cost density gradient maker [PDF]
Chemistry Teacher InternationalMicroplastics are now ubiquitous in the environment and are even considered “technofossils” of the Anthropocene. Given their omnipresence and potential impact, identifying and analyzing these particles becomes increasingly crucial.
Majcen Alina +5 more
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