Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation [PDF]
The World Journal of Men's Health, 2021 Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated.
Gabriela Hernández-Silva +7 more
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Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures [PDF]
MethodsX, 2023 Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay +4 more
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Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90% [PDF]
MethodsX, 2021 Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa +8 more
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Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]
Reproductive Medicine and BiologyPurpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano +15 more
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Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation [PDF]
BMC Research Notes, 2023 Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza +5 more
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Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia [PDF]
Life, 2021 In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler +5 more
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Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]
International Journal of Fertility and Sterility, 2020 Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini +6 more
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The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa [PDF]
BMC Veterinary Research, 2022 Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls.
Maria Eberhardt +5 more
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Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity [PDF]
International Journal of General MedicineZi-Na Wen, Li Duan, Yong Chen, Qing-Hong Qiu, Gang Liu, Ning Luo, Peng-Hao Li, Er-Po Tian, Ren-Shan Ge Andrology Laboratory, Sichuan Jinxin Xinan Women and Children Hospital, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Ren-Shan Ge ...
Wen ZN +8 more
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A density gradient centrifugation method for rapid separation of nanoTiO2 and TiO2 aggregates from microalgal cells in complex mixtures with mercury [PDF]
MethodsX, 2020 In natural environment, the microorganisms are exposed to complex mixtures of contaminants, including manufactured nanoparticles and their aggregates.
Mengting Li, Vera I. Slaveykova
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