Results 31 to 40 of about 226,785 (298)

Different Methods for Seminal Plasma Removal and Sperm Selection on the Quality and Fertility of Collared Peccary Sperm

Animals, 2023
Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a ...
Maria V. O. Santos, Andréia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira   +7 more
doaj   +1 more source

Preparation of purified peste des petits ruminants (PPR) virus antigen from a local isolate of Bangladesh

Fundamental and Applied Agriculture, 2019
Peste des petits ruminants (PPR) is the number one killer disease of small ruminants. The disease causes severe losses to small ruminant production and is presently considered as one of the major threats to about 22 million small ruminant population of ...
Ashrafun Naher Luna, Fardina Sultana Sumi, Jahan Ara Begum, Md. Mushfiqur Rahman, Mohammad Rafiqul Islam, Emdadul Haque Chowdhury   +5 more
doaj   +1 more source

In vitro cultivation of rat bone marrow mesenchymal stem cells and establishment of pEGFP/Ang-1 transfection method

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang, Long Wang, Shu-Li Zhao, Wei Xu   +3 more
doaj   +1 more source

Elimination of bacteria from semen using a combination of density gradient centrifugation and swim-up

Journal of Men's Health, 2022
Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun   +6 more
doaj   +1 more source

Timing of semen cryopreservation: before or after processing? [PDF]

Revista Brasileira de Ginecologia e Obstetrícia
Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta   +4 more
doaj   +1 more source

IMPACT OF PREPARATION USING CONVENTIONAL AND MODIFIED DENSITY GRADIENT CENTRIFUGATION METHODS ON SPERM CONCENTRATION, MOTILITY AND NUMBER OF NORMAL MOTILE SPERM RECOVERY (NMSR)

Folia Medica Indonesiana, 2017
Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques.
Rina Yudiwati, MPBD Pramesti, Agustinus Agustinus, E Pradana, Bambang Purwanto   +4 more
doaj   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Ion-exchange chromatography purification of extracellular vesicles

BioTechniques, 2017
Despite numerous studies, isolating pure preparations of extracellular vesicles (EVs) has proven challenging. Here, we compared ion-exchange chromatography (IEC) to the widely used sucrose density gradient (SDG) centrifugation method for the purification
Maja Kosanović, Bojana Milutinović, Sanja Goč, Ninoslav Mitić, Miroslava Janković   +4 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source