Results 131 to 140 of about 39,457 (305)
Intracellular compartmentation of deoxycytidine nucleotide pools in S phase mouse 3T3 fibroblasts
, 1989 We labeled mouse 3T3 fibroblasts, synchronized in G0 or S phase, from [3H]cytidine or [3H]deoxycytidine and measured the flow of isotope into and through deoxycytidine nucleotide pools, including the two deoxyliponucleotides dCDP choline and dCDP ...
Reichard, Peter,, Spyrou, Giannis,
core
Journal of Integrative Plant Biology, EarlyView.Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang +7 more
wiley +1 more source
Journal of Integrative Plant Biology, EarlyView.Pentatricopeptide repeat proteins coordinate nucleus‐organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA ...
Mingming Wu +7 more
wiley +1 more source
Effects of 5-aza-2′-deoxycytidine on PEPP2 expression.
, 2016 PL21 and HL60 cells, and PBMNCs from HD were incubated with 200 nM of 5-aza-2′-deoxycytidine for 72 hours. RNA was extracted and RT-PCR (A) or quantitative PCR (B) was performed. Cells were also examined for protein expression by immunohistochemistry (C).
Hiroyuki Aburatani (162634) +11 more
core +1 more source
Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns
Journal of Integrative Plant Biology, EarlyView.Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng +5 more
wiley +1 more source
eGFP expression increased after 5-Aza-2′-Deoxycytidine treatment.
, 2012 A) Bright field (upper row) and fluorescent (bottom row) images of D1 sorted corrected cells at different experimental time (scale bar: 150 µm).
Massimo Sanchez (183600) +7 more
core +1 more source
Journal of Integrative Plant Biology, EarlyView.Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen +8 more
wiley +1 more source
Solvent effects on blue shifted improper hydrogen bond of C-H···O in deoxycytidine isomers
, 2010 Solvent effects on blue shifted improper hydrogen bond of C-H···O in deoxycytidine ...
Fangfang Chen (13063851) +2 more
core
, 2018 5-(Hydroxymethyl)-2′-deoxycytidine (5-hmdC), 5-(formyl)-2′-deoxycytidine (5-fodC), and 5-(carboxyl)-2′-deoxycytidine (5-cadC) are crucial intermediate products of the DNA demethylation pathway, which can also act as potential biomarkers reflecting the ...
Dantong Liu (4922356) +7 more
core +1 more source
Plant, Cell &Environment, EarlyView.ABSTRACT Bacteriophages impact soil bacteria through lysis, altering the availability of organic carbon and plant nutrients. However, the magnitude of nutrient uptake by plants from lysed bacteria remains unknown, partly because this process is challenging to investigate in the field.
Vlastimil Novak +10 more
wiley +1 more source