Results 141 to 150 of about 14,531 (182)

A Continuous Spectrophotometric Assay for Thymidine and Deoxycytidine Kinases

Analytical Biochemistry, 1998
We have developed a continuous spectrophotometric assay for thymidine and deoxycytidine kinase activities by coupling nucleoside 5'-monophosphate formation to a methylation reaction which generates a product absorbing at 340 nm. With thymidine kinase, we used the alternate substrate deoxyuridine and coupled the reaction to thymidylate synthase.
Y, Liu, D V, Santi
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5-Fluorocytosine derivatives as inhibitors of deoxycytidine kinase

Bioorganic & Medicinal Chemistry Letters, 2009
A series of potent piperidine-linked cytosine derivatives were prepared as inhibitors of deoxycytidine kinase (dCK). Compound 9h was discovered to be a potent inhibitor of dCK and shows a good combination of cellular potency and pharmacokinetic parameters.
James E, Tarver, Theodore C, Jessop, Marianne, Carlsen, David J, Augeri, Qinghong, Fu, Jason P, Healy, Alexander, Heim-Riether, Amy, Xu, Jerry A, Taylor, Min, Shen, Philip E, Keyes, S, David Kimball, Xuan-Chuan, Yu, Maricar, Miranda, Qingyun, Liu, Jonathan C, Swaffield, Amr, Nouraldeen, Alan G E, Wilson, Rick, Finch, Kanchan, Jhaver, Ann Marie Digeorge, Foushee, Steve, Anderson, Tamas, Oravecz, Kenneth G, Carson   +23 more
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THE NMR CONFORMATION STUDY OF THE COMPLEXES OF DEOXYCYTIDINE KINASE (dCK) AND 2′-DEOXYCYTIDINE/2′-DEOXYADENOSINE

Nucleosides, Nucleotides and Nucleic Acids, 2001
The structures of the bound 13C/2H double-labelled 2'(R/S), 5'(R/S)-2H2-1',2',3',4',5'-13C5-2'-deoxyadenosine and the corresponding 2'-deoxycytidine moieties in the complexes with human deoxycytidine kinase (dCK) have been characterized for the first time by the solution NMR spectroscopy, using Transferred Dipole-Dipole Cross-correlated Relaxation and ...
T, Maltseva, E, Usova, S, Eriksson, J, Milecki, A, Földesi, J, Chattopadhayaya   +5 more
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Selective activation of deoxycytidine kinase by thymidine-5′-thiosulphate and release by deoxycytidine in human lymphocytes

Biochemical Pharmacology, 2003
Deoxycytidine kinase (dCK) catalyses the rate-limiting step of the salvage of three natural deoxyribonucleosides as well as several therapeutic nucleoside analogues, which in turn can enhance its enzymatic activity [Biochem Pharmacol 56 (1998) 1175], improving the efficacy of the cytostatic therapy.
Gergely, Keszler, Karoly, Szikla, Zygmunt, Kazimierczuk, Tatjana, Spasokoukotskaja, Maria, Sasvari-Szekely, Maria, Staub   +5 more
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Nucleotide specificity of human deoxycytidine kinase.

Molecular Pharmacology, 1992
The ability of deoxycytidine kinase (dCK) to phosphorylate 2'-deoxycytidine (dCyd) and its analogs in the presence of eight nucleoside triphosphates (NTPs), simulating the cellular milieu, was investigated. Using highly purified dCK from MOLT-4 T lymphoblasts, Km and Vmax values were determined for the phosphorylation of dCyd in the presence of ...
D S, Shewach, K K, Reynolds, L, Hertel
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Activation of Deoxycytidine Kinase by Various Nucleoside Analogues

1998
The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells.
T, Spasokoukotskaja, M, Sasvári-Székely, L, Hullán, F, Albertioni, S, Eriksson, M, Staub   +5 more
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Unexpected Immunohistochemical Localization of Deoxycytidine Kinase

Clinical Cancer Research, 2006
To the Editor: We read with great interest the April 15, 2006 article by Sebastiani et al. reporting immunohistochemical evaluation of deoxycytidine kinase (dCK) in 44 pancreatic cancer patients, which found that most cases (40 of 44) were positive for dCK ([1][1]).
Lorelei Johnson, Raymond Lai, Cheryl Santos, Laith Dabbagh, John Mackey   +4 more
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Cloning of the Dck gene encoding rat deoxycytidine kinase

Gene, 1994
In order to study the mutational inactivation of deoxycytidine kinase (Dck) in a rat model for acute myeloid leukemia we have cloned the complete coding region of the rat Dck gene. Using primers chosen from the human Dck cDNA sequence, we obtained a rat-specific probe via PCR and used it to isolate two clones from a rat lymphocyte cDNA library. The ORF
A P, Stegmann, M W, Honders, R, Willemze, J E, Landegent   +3 more
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Thymidine kinase and deoxycytidine kinase in HIV-infected children.

The new microbiologica, 2009
It has been demonstrated that HIV infection may affect the levels of thymidine kinase (TK) and deoxycytidine kinase (dCK) in peripheral blood mononuclear cells from HIV infected adults. The aim of this study was to examine the effect of HIV infection and/or antiretroviral therapy on the activity of the above enzymes in HIV-infected children.
FALASCA, FRANCESCA, MAIDA P, BONI A, BERNARDI S, CURSI L, RIZZO B, ANTONELLI, Guido, GATTINARA GC, TURRIZIANI, Ombretta   +8 more
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5'-Substituted 2'-Deoxycytidines as Non-Substrate Inhibitors of Human Deoxycytidine Kinase

Nucleosides, Nucleotides and Nucleic Acids, 1995
Abstract Several 5′-substituted analogues of 2′-deoxycytidine (dC), including 5′-amino2′,5′-dideoxycytidine (5′-amino-ddC), 5′-azido-2′,5′-dideoxycytidine (5′-azido-ddC) and 5′-O-ethyl-2′-deoxycytidine (5′-O-ethyl-dC), are non-substrate inhibitors of human dC kinase.
K. Krawiec, B. Kierdaszuk, D. Shugar
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