Results 1 to 10 of about 69,682 (133)
BioTechniques, 1999 Human tumor samples were screened for point mutations by adapting a mobilityshift assay to automated DNA sizing. This screen identifies the type of point mutation and relative amount of mutated DNA sequences present in a sample. Test samples having known
L.A. Marcelino +6 more
doaj +2 more sources
Study of the method of spinal cord neuron culture in Sprague–Dawley rats
Ibrain, Volume 9, Issue 3, Page 270-280, Fall 2023., 2023 In this study, the separation and purification methods of spinal cord neuron (SPN) culture, that is, the cytarabine (Ara‐C) inhibition method, the differential velocity adherent method, and natural growth medium for neurons, were compared. The neuron‐specific medium can be grown naturally and cultivated to obtain SPNs of higher purity.
Yi‐Fei Sun +3 more
wiley +1 more source
APOBEC3G protects the genome of human cultured cells and mice from radiation‐induced damage
The FEBS Journal, Volume 290, Issue 7, Page 1822-1839, April 2023., 2023 A3G protects mice from IR‐induced damage and promotes accurate DNA double‐strand breaks repair in cultured cells. Transgenic mice expressing A3G successfully survived lethal irradiation, whereas wild‐type controls quickly succumbed to radiation syndrome.
Yelena Britan‐Rosich +11 more
wiley +1 more source
A compendium of single extracellular vesicle flow cytometry
Journal of Extracellular Vesicles, Volume 12, Issue 2, February 2023., 2023 Abstract Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more ...
Joshua A. Welsh +23 more
wiley +1 more source
Functionalized electrodes embedded in nanopores: read‐out enhancement?
Chemistry – An Asian Journal, Volume 18, Issue 1, January 3, 2023., 2023 Nanogaps functionalized with tiny diamonds can enhance the read‐out of nanopores and detect mutations. Abstract In this review, functionalized nanogaps embedded in nanopores are discussed in view of their high biosensitivity in detecting biomolecules, their length, type, and sequence.
Maria Fyta
wiley +1 more source
Z‐nucleic acids: Uncovering the functions from past to present
European Journal of Immunology, Volume 52, Issue 11, Page 1700-1711, November 2022., 2022 This article summarizes the research on Z‐nucleic acid historically and highlights the milestones in this field, including the discovery, characterization of the Z‐nucleic acids and their biological functions mediated by interacting with Z‐nucleic acid binding proteins such as ZBP1, ADAR1, and so on.
Qiannan Tang
wiley +1 more source
ChemBioChem, Volume 23, Issue 11, June 3, 2022., 2022 A bioinorganic perspective on the AlkB as well as the TET enzyme families, Fe2+/α‐ketoglutarate‐dependent dioxygenases involved in nucleic acid repair and processing, and epigenetic gene regulation is presented in this review. Important spectroscopic techniques which can be useful for in vitro examination of the structure and formation of those ...
David Schmidl +4 more
wiley +1 more source
Biotechnology and Bioengineering, Volume 119, Issue 1, Page 145-161, January 2022., 2022 Development and application of powerful target metabolite profiling methodology to monitor how primary metabolism support precursors and energy for heterologous secondary metabolite production in Streptomyces superhost strains Abstract Microbial superhost strains should provide an ideal platform for the efficient homologous or heterologous phenotypic ...
Kanhaiya Kumar, Per Bruheim
wiley +1 more source
Epigenetics: a catalyst of plant immunity against pathogens
New Phytologist, Volume 233, Issue 1, Page 66-83, January 2022., 2022 Summary The plant immune system protects against pests and diseases. The recognition of stress‐related molecular patterns triggers localised immune responses, which are often followed by longer‐lasting systemic priming and/or up‐regulation of defences. In some cases, this induced resistance (IR) can be transmitted to following generations.
Adam Hannan Parker +2 more
wiley +1 more source
Oxidative Medicine and Cellular Longevity, Volume 2021, Issue 1, 2021., 2021 Currently, traditional cancer therapy still falls far short of expectations. However, a variety of invasive cancers that are resistant to chemotherapy (such as platinum drugs, one of the most applied antineoplastics in clinic) and targeted agents are susceptible to ferroptosis.
Yue Cheng +4 more
wiley +1 more source