Results 11 to 20 of about 192,494 (228)
Deoxyribonuclease Treatment Improves the Homogeneity of Single-Stranded DNA Preparations
BioTechniques, 1997 The isolation of single-stranded (ss) phagemid DNA using standard protocols often results in impure preparations, which contain undesirable quantities of chromosomal and/or double-stranded (ds) phagemid DNA.
T.K. Aliev +3 more
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Deoxyribonuclease activities of rumen bacteria from the genus Prevotella
Acta Agriculturae Slovenica, 1999 Twenty-nine strains belonging to four species of the anaerobic bacterial genus Prevotella were tested for deoxyribonuclease activity against the nonmethylated l phage DNA and circular plasmid pBR322 DNA.
Tomaž ACCETTO, Gorazd AVGUŠTIN
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THE HETEROGENEITY OF PANCREATIC DEOXYRIBONUCLEASE
Canadian Journal of Biochemistry and Physiology, 1962 The action of crystalline pancreatic deoxyribonuclease on sodium oligonucleotides in the presence of manganous ions has been studied and a pH optimum of 6.6 observed. Inhibition of the enzyme activity by increased ionic strength of the digest occurred. The liberation of products soluble in uranyl acetate – trichloroacetate was found to vary with enzyme
G C, BECKING, R O, HURST
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Extracellular DNA and Deoxyribonuclease Activity as Prognostic Markers in Sepsis
BiomedicinesBackground/Objectives: Sepsis is characterized by a dysregulated immune response to infection and is associated with high lethality. Extracellular DNA (ecDNA) has drawn significant interest as a damage-associated molecular pattern because of its ...
Monika Janíková +8 more
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The Specificity of Pancreatic Deoxyribonuclease [PDF]
European Journal of Biochemistry, 1973 The kinetics of degradation of calf thymus DNA by pancreatic DNAase has been investigated with the main purpose of providing the background for studying the specificity of the enzyme and its use in investigations of nucleotide sequences in DNA. The relationships between the hyperchromic shift or acid solubility and the reciprocal average degree of ...
S. D. Ehrlich +2 more
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An injectable hydrogel to disrupt neutrophil extracellular traps for treating rheumatoid arthritis
Drug Delivery, 2023 Rheumatoid arthritis (RA), an autoimmune disease, is characterized by inflammatory cell infiltration that damages cartilage, disrupts bone, and impairs joint function.
Nan Wang +3 more
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Deoxyribonucleases and Their Applications in Biomedicine [PDF]
Biomolecules, 2020 Extracellular DNA, also called cell-free DNA, released from dying cells or activated immune cells can be recognized by the immune system as a danger signal causing or enhancing inflammation. The cleavage of extracellular DNA is crucial for limiting the inflammatory response and maintaining homeostasis.
Lucia Lauková +4 more
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Encyclopedia, 2021 The deoxyribonuclease 1 (Dnase1) family is a key family of endonucleases that degrades DNA. Loss of Dnase1 family function causes several diseases where the host’s immune system targets the host, such as systemic lupus erythematosus, hypocomplementemic ...
Minal Engavale +6 more
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The role of the host—Neutrophil biology
Periodontology 2000, EarlyView., 2023 Abstract Neutrophilic polymorphonuclear leukocytes (neutrophils) are myeloid cells packed with lysosomal granules (hence also called granulocytes) that contain a formidable antimicrobial arsenal. They are terminally differentiated cells that play a critical role in acute and chronic inflammation, as well as in the resolution of inflammation and wound ...
Iain L. C. Chapple +4 more
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Deoxyribonucleases of Pneumococcus
Journal of Biological Chemistry, 1967 Abstract Two enzymes with deoxyribonuclease activity were isolated from Pneumococcus. One of these, an endonuclease active on both native and denatured deoxyribonucleic acid, produced 5'-phosphate-terminated fragments which, even after extensive degradation, were larger than mononucleotides.
S, Lacks, B, Greenberg
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