Results 81 to 90 of about 322,846 (227)

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

First 20 Years of Orbitrap Mass Spectrometry as the Mainstream Analytical Technique

Mass Spectrometry Reviews, EarlyView.
ABSTRACT This review traces the first 20 years of Orbitrap mass spectrometry as a mainstream high‑resolution and accurate‑mass (HR/AM) technology. It outlines the historical development of the Orbitrap analyzer, the evolution of major instrument families, and the key technological innovations that enabled its widespread adoption. Particular emphasis is
Alexander Makarov
wiley   +1 more source

Recent Advances in Top‐Down Proteomics for Single‐Cell Research

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Individual cells have distinctive molecular characteristics, including biologically relevant proteoforms. Although single‐cell (SC) molecular omics offer unprecedented insights into cellular heterogeneity and function, the characterization of proteoforms in SCs remains an uncharted territory.
Jake A. Melby, Pei Su, Fabio P. Gomes
wiley   +1 more source

Examination of the Molecular Mass of Polyhydroxypolyamides [PDF]

, 2010
The work described in this thesis investigated the use of size exclusion chromatography (SEC) to fractionate a variety of different polyhydroxypolyamide (PHPA) samples for analysis by matrix assisted laser desorption ionisation-time of flight ...
Faville, Martyn
core  

Imaging of Human Lens Lipids by Desorption Electrospray Ionization Mass Spectrometry [PDF]

, 2010
The lipid composition of the human lens is distinct from most other tissues in that it is high in dihydrosphingomyelin and the most abundant glycerophospholipids in the lens are unusual 1-O-alkyl-ether linked phosphatidylethanolamines and ...
Wu, C., Mitchell, T. W., Cooks, R. Graham, Deeley, J. M., Todd W. Mitchell, Chunping Wu, R. Graham Cooks, Panhuis, M. I. H., Panhuis, Marc in het, Xiangjia Zhu, Zhu, Xiangjia, Zhu, X., Roger J. W. Truscott, Truscott, R. J. W., Cooks, R. G., Truscott, Roger J.W., Wu, Chunping, Shane R. Ellis, Stephen J. Blanksbya, Ellis, Shane R., Mitchell, Todd W., Jane M. Deeley, Deeley, Jane M., Marc in het Panhuis, Ellis, S. R., Blanksby, Stephen J.   +25 more
core   +1 more source

Use of analytical strategies to understand spatial chemical variation in bacterial surface communities

Journal of Bacteriology
Not only do surface-growing microbes such as biofilms display specific traits compared to planktonic cells, but also they display many heterogeneous behaviors over many spatial and temporal contexts.
Abigail A. Weaver, Joshua D. Shrout
doaj   +1 more source

Solid-Phase Extraction Strategies to Surmount Body Fluid Sample Complexity in High-Throughput Mass Spectrometry-Based Proteomics

Journal of Analytical Methods in Chemistry, 2015
For large-scale and standardized applications in mass spectrometry- (MS-) based proteomics automation of each step is essential. Here we present high-throughput sample preparation solutions for balancing the speed of current MS-acquisitions and the time ...
Marco R. Bladergroen, Yuri E. M. van der Burgt   +1 more
doaj   +1 more source

Mass Spectrometry Insights Into Post‐Translational Modifications in Extracellular Vesicles

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are membrane‐enclosed structures secreted by virtually all living cells, serving as essential mediators of intercellular communication in both physiological and pathological processes. There is growing interest in their potential applications as biomarkers, therapeutic targets, and drug delivery systems, which ...
Dávid Virág, Krisztián Márk Karvaly, Alexandra Molnár, Mirjam Balbisi, Júlia Németh, Lilla Turiák   +5 more
wiley   +1 more source

Advancing proteomics with ion/ion chemistry Mass Spectrometry For Proteomics Analysis

BioTechniques, 2006
Mass spectrometers, instruments that use electric and/or magnetic fields to measure a gas-phase ion's mass-to-charge ratio (m/z), are used in a wide variety of applications—with the field having a reputation for providing good sensitivity and high ...
David M. Good, Joshua J. Coon
doaj   +1 more source

Hydrogen Is the Superior Nebulization Gas for Desorption and Electrospray Ionization

Analytical Chemistry
A previous comparative study between helium and nitrogen as nebulizing and desolvation gases in electrospray ionization (ESI) and desorption electrospray ionization (DESI) found that the signal responses of compounds of varying sizes and polarities were improved. Here, an expanded selection of nebulizing gases was evaluated to investigate mechanisms of
Bincy Binny, George Joseph, Andre R. Venter   +2 more
openaire   +2 more sources