Results 31 to 40 of about 14,908,548 (354)

In silico prediction of post-translational modifications in therapeutic antibodies

mAbs, 2022
Monoclonal antibodies are susceptible to chemical and enzymatic modifications during manufacturing, storage, and shipping. Deamidation, isomerization, and oxidation can compromise the potency, efficacy, and safety of therapeutic antibodies.
Shabdita Vatsa
doaj   +1 more source

Characterization and Modeling of Reversible Antibody Self-Association Provide Insights into Behavior, Prediction, and Correction

Antibodies, 2021
Reversible antibody self-association, while having major developability and therapeutic implications, is not fully understood or readily predictable and correctable.
Carl Mieczkowski, Alan Cheng, Thierry Fischmann, Mark Hsieh, Jeanne Baker, Makiko Uchida, Gopalan Raghunathan, Corey Strickland, Laurence Fayadat-Dilman   +8 more
doaj   +1 more source

A library approach for the de novo high-throughput isolation of humanized VHH domains with favorable developability properties following camelid immunization

mAbs, 2023
In this study, we generated a novel library approach for high throughput de novo identification of humanized single-domain antibodies following camelid immunization. To achieve this, VHH-derived complementarity-determining regions-3 (CDR3s) obtained from
Paul Arras, Han Byul Yoo, Lukas Pekar, C. Schröter, Thomas Clarke, Simon Krah, Daniel Klewinghaus, Vanessa Siegmund, Andreas Evers, Stefan Zielonka   +9 more
semanticscholar   +1 more source

Machine learning prediction of antibody aggregation and viscosity for high concentration formulation development of protein therapeutics

mAbs, 2022
Machine learning has been recently used to predict therapeutic antibody aggregation rates and viscosity at high concentrations (150 mg/ml). These works focused on commercially available antibodies, which may have been optimized for stability.
Pin-Kuang Lai, Austin Gallegos, Neil Mody, Hasige A. Sathish, Bernhardt L. Trout   +4 more
doaj   +1 more source

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

mAbs, 2023
Antibody drugs should exhibit not only high-binding affinity for their target antigens but also favorable physicochemical drug-like properties. Such drug-like biophysical properties are essential for the successful development of antibody drug products ...
Hristo L. Svilenov, Paolo Arosio, Tim Menzen, Peter Tessier, Pietro Sormanni   +4 more
doaj   +1 more source

Testis Development [PDF]

Endocrine Reviews, 2018
Production of sperm and androgens is the main function of the testis. This depends on normal development of both testicular somatic cells and germ cells. A genetic program initiated from the Y chromosome gene sex-determining region Y (SRY) directs somatic cell specification to Sertoli cells that orchestrate further development.
Juho-Antti Mäkelä, Jaakko J Koskenniemi, Helena E Virtanen, Jorma Toppari   +3 more
openaire   +2 more sources

PEP-Patch: Electrostatics in Protein–Protein Recognition, Specificity, and Antibody Developability

bioRxiv, 2023
The electrostatic properties of proteins arise from the number and distribution of polar and charged residues. Due to their long-ranged nature, electrostatic interactions in proteins play a critical role in numerous processes, such as molecular ...
Franz Waibl, Nancy D. Pomarici, Valentin J. Hoerschinger, J. R. Loeffler, C. Deane, G. Georges, H. Kettenberger, M. Fernández-Quintero, K. Liedl   +8 more
semanticscholar   +1 more source

An accelerated surface-mediated stress assay of antibody instability for developability studies

mAbs, 2020
High physical stability is required for the development of monoclonal antibodies (mAbs) into successful therapeutic products. Developability assays are used to predict physical stability issues such as high viscosity and poor conformational stability ...
Marie R.G. Kopp, Adriana-Michelle Wolf Pérez, Marta Virginia Zucca, Umberto Capasso Palmiero, Brigitte Friedrichsen, Nikolai Lorenzen, Paolo Arosio   +6 more
doaj   +1 more source

Guiding diffusion models for antibody sequence and structure co-design with developability properties

bioRxiv, 2023
Recent advances in deep generative methods have allowed antibody sequence and structure co-design. This study addresses the challenge of tailoring the highly variable complementarity-determining regions (CDRs) in antibodies to fulfill developability ...
Amelia Villegas-Morcillo, Jana M. Weber, M. Reinders   +2 more
semanticscholar   +1 more source

Profiling the Biophysical Developability Properties of Common IgG1 Fc Effector Silencing Variants

Antibodies, 2023
Therapeutic antibodies represent the most significant modality in biologics, with around 150 approved drugs on the market. In addition to specific target binding mediated by the variable fragments (Fvs) of the heavy and light chains, antibodies possess ...
R. Pejchal, A. Cooper, Michael E. Brown, M. Vásquez, E. Krauland   +4 more
semanticscholar   +1 more source