Results 51 to 60 of about 28,160 (153)
Legionella pneumophila modulates the host cytoskeleton by an effector of transglutaminase activity
mLife, EarlyView.Abstract The bacterial pathogen Legionella pneumophila delivers more than 330 effector proteins into host cells through its Dot/Icm type IV secretion system (T4SS) to facilitate its intracellular replication. A number of these effectors modulate organelle trafficking pathways to create a membrane‐bound niche called the Legionella‐containing vacuole ...
Yan Liu, Yao Liu, Zhao‐Qing Luo
wiley +1 more source
Analysis of Chemotaxis in Dictyostelium [PDF]
, 2011 Dictyostelium discoideum is an excellent model organism for the study of directed cell migration, since Dictyostelium cells show robust chemotactic responses to the chemoattractant cAMP. Many powerful experimental tools are applicable, including forward and reverse genetics, biochemistry, microscopy, and proteomics.
Chuan Hsiang Huang +3 more
openaire +3 more sources
Small Structures, EarlyView.Seeded growth onto aggregated seed crystallites of a unimer mixture of polymers containing one crystalline block presents fascinating similarities with the aggregation of Dictyostelium discoideum amoebae into slime molds. The mixture subdivides in two groups to form compartmentalized nanoflowers with thin petals surrounding a thicker central section ...
Jiantian Zhao +9 more
wiley +1 more source
A recombinant antibody toolbox for Dictyostelium discoideum
BMC Research Notes, 2020 Objective The amoeba Dictyostelium discoideum has been a valuable model organism to study numerous facets of eukaryotic cell biology, such as cell motility, cell adhesion, macropinocytosis and phagocytosis, host–pathogen interactions and multicellular ...
Wanessa C. Lima +2 more
doaj +1 more source
CRISPR Toolbox for Genome Editing in Dictyostelium
Frontiers in Cell and Developmental Biology, 2021 The development of new techniques to create gene knockouts and knock-ins is essential for successful investigation of gene functions and elucidation of the causes of diseases and their associated fundamental cellular processes.
Kensuke Yamashita +3 more
doaj +1 more source
A signal transduction blind spot: the function of adenylyl cyclase transmembrane domains
The FEBS Journal, EarlyView.Transmembrane adenylyl cyclases (tmACs) are essential for synthesising the universal second messenger cyclic AMP. All tmACs share a common topology that includes a large 12‐helix transmembrane (TM) domain. This domain accounts for ~ 35% of the coding sequence but its function is still an open question.
Ryan S. Dowsell, Matthew G. Gold
wiley +1 more source
Inference of the drivers of collective movement in two cell types: Dictyostelium and melanoma [PDF]
, 2016 Collective cell movement is a key component of many important biological processes, including wound healing, the immune response and the spread of cancers.
Ferguson, Elaine A. +3 more
core +2 more sources
Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium
Cells, 2019 In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium.
Tetsuya Muramoto +3 more
doaj +1 more source
Luminal Phospholipase D Attacks Bacterial Membranes in Dictyostelium discoideum Phagosomes
Molecular Microbiology, EarlyView.This study shows that PldX, a luminal Phospholipase D, is necessary for the efficient destruction of ingested Klebsiella pneumoniae bacteria in Dictyostelium discoideum phagosomes. PldX and the AlyL lysozyme together attack the bacterial membrane and permeabilize it. To date, the molecular substrate of PldX remains to be determined. ABSTRACT Phagocytic
Otmane Lamrabet +6 more
wiley +1 more source
Local modulation of chemoattractant concentrations by single cells: dissection using a bulk-surface computational model [PDF]
, 2016 Chemoattractant gradients are usually considered in terms of sources and sinks that are independent of the chemotactic cell. However, recent interest has focused on ‘self-generated’ gradients, in which cell populations create their own local gradients as
Insall, R.H., Mackenzie, J.A., Nolan, M.
core +1 more source