Results 131 to 140 of about 6,190 (152)
Some of the next articles are maybe not open access.
Use of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands
2016Identification of small molecules that interact specifically with the ligand-binding domains (LBDs) of nuclear receptors (NRs) can be accomplished using a variety of methodologies. Here, we describe the use of differential scanning fluorimetry to identify these ligands, a technique that requires no modification or derivatization of either the protein ...
Kara A, DeSantis, Jeffrey L, Reinking
openaire +2 more sources
Protein stability in an ionic liquid milieu: on the use of differential scanning fluorimetry
Physical Chemistry Chemical Physics, 2011Protein stability is a major bottleneck in the biotechnological application of ionic liquid-containing solvents, either in the frame of biocatalysis or protein storage. Herein, differential scanning fluorimetry was successfully implemented as a high throughput method to fast scan the impact of a number of cholinium-based ionic liquids on the stability ...
João V, Rodrigues +4 more
openaire +2 more sources
Fluorimetry and differential scanning calorimetry analysis of ionomers with low Eu3+ contents
Journal of Polymer Science, Part B: Polymer Physics, 1997Fluorimetry and differential scanning calorimetry have been used to characterize ionomers that were synthesized by copolymerization of methyl methacrylate, methacrylic acid, and europium methacrylate (EMA). Under excitation of UV light at 375 nm no self-quenching was found in fluorescence of EMA-containing ionomers at 615 nm within the Eu3 ...
exaly +2 more sources
2011
Studies of the structural changes invoked in proteins by the binding of the glycosaminoglycan (GAG) polysaccharide portion of proteoglycans are of increasing importance to research in a wide range of fields, from biochemistry and molecular biology to biotechnology and medicine.
Katarzyna A, Uniewicz +6 more
openaire +2 more sources
Studies of the structural changes invoked in proteins by the binding of the glycosaminoglycan (GAG) polysaccharide portion of proteoglycans are of increasing importance to research in a wide range of fields, from biochemistry and molecular biology to biotechnology and medicine.
Katarzyna A, Uniewicz +6 more
openaire +2 more sources
Journal of Pharmaceutical and Biomedical Analysis, 2013
When formulating a biopharmaceutical protein, its stability in the liquid state is critical. In addition, when preparing biological reference materials the stability, both when lyophilised and after reconstitution, needs to be determined. In order to optimise the stability in aqueous conditions (as indicated by Tmelt or denaturation point) the impact ...
Paul Matejtschuk, Craig Thelwell
exaly +3 more sources
When formulating a biopharmaceutical protein, its stability in the liquid state is critical. In addition, when preparing biological reference materials the stability, both when lyophilised and after reconstitution, needs to be determined. In order to optimise the stability in aqueous conditions (as indicated by Tmelt or denaturation point) the impact ...
Paul Matejtschuk, Craig Thelwell
exaly +3 more sources
Screening of DNA G-quadruplex stabilizing ligands by nano differential scanning fluorimetry
The Analyst, 2019A proof of principle study on the use of nanoDSF as a screening tool for G-quadruplex targeting compounds.
Pagano, Bruno +4 more
openaire +3 more sources
Nature Protocols, 2007
Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye with affinity for hydrophobic parts of the protein, which are exposed as the protein ...
Frank H, Niesen +2 more
openaire +2 more sources
Differential scanning fluorimetry (DSF) is a rapid and inexpensive screening method to identify low-molecular-weight ligands that bind and stabilize purified proteins. The temperature at which a protein unfolds is measured by an increase in the fluorescence of a dye with affinity for hydrophobic parts of the protein, which are exposed as the protein ...
Frank H, Niesen +2 more
openaire +2 more sources
The Journal of Physical Chemistry Letters, 2017
Thermodynamic properties of protein unfolding have been extensively studied; however, the methods used have typically required significant preparation time and high protein concentrations. Here we present a facile, simple, and parallelized differential scanning fluorimetry (DSF) method that enables thermodynamic parameters of protein unfolding to be ...
Thaiesha A. Wright +3 more
openaire +2 more sources
Thermodynamic properties of protein unfolding have been extensively studied; however, the methods used have typically required significant preparation time and high protein concentrations. Here we present a facile, simple, and parallelized differential scanning fluorimetry (DSF) method that enables thermodynamic parameters of protein unfolding to be ...
Thaiesha A. Wright +3 more
openaire +2 more sources
Stability of proteins and viruses determined by differential scanning fluorimetry
2016Differential scanning fluorimetry (DSF) is readily employed high-throughput method for probing protein stability. Typically, DSF monitors thermal unfolding of proteins using a fluorescent reporter dye (e.g. Sypro Orange) and a real-time PCR instrument[1]. Recently, a new method, nanoDSF, was developed which enables measuring of intrinsic tryptophan and
Sviben, Dora +4 more
openaire +2 more sources
Evaluation of compound selectivity of aldo-keto reductases using differential scanning fluorimetry
Journal of Biochemistry, 2016Inhibitors of AKR1B10 belonging to the aldo-keto reductase (AKR) superfamily are considered promising candidates for anti-cancer drugs. AKR1B1, a structurally similar isoform of AKR1B10, is involved in glucose metabolism. Thus, selective inhibition of AKR1B10 is required for the development of anti-cancer drugs.
Aurangazeb, Kabir +5 more
openaire +2 more sources