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Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein
2020Differential scanning fluorimetry (DSF) can be used to detect the binding of a small molecule ligand to a purified target protein. Upon binding with certain ligands, the protein can be stabilized from thermal denaturation. DSF uses a fluorescent dye and Real-Time PCR instrument to detect the unfolding process of proteins during thermal denaturation ...
Xiaohui, Li, Chunhua, Zhang
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Differential scanning fluorimetry (DSF) is a versatile and accessible technique for probing the thermal stability of peptide-major histocompatibility complexes (pMHCs). Understanding the thermal transition midpoint (Tm) between the folded and unfolded states of pMHCs provides critical insights into their structural stability, which is essential for ...
Holly Anne, Martin, Lance M, Hellman
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Holly Anne, Martin, Lance M, Hellman
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Characterization of human Akt1-lipid interactions by label-free differential scanning fluorimetry
Understanding the role of lipids in protein function is key to obtaining details about cellular signaling pathways. Among the various methods available, label-free differential scanning fluorimetry has several advantages. This technique uses the intrinsic fluorescence of tryptophan and tyrosine residues, requires minute sample amounts, enables rapid ...Obada, Bahra +2 more
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BPS2025 - Differential scanning fluorimetry and computational studies of SEX4
Biophysical JournalIsabelle E. Crampton +3 more
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Kinase Inhibitor Selectivity Profiling Using Differential Scanning Fluorimetry
Methods in Molecular Biology, 2012Oleg Fedorov +2 more
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