Results 161 to 170 of about 3,651,834 (302)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Identification of Differentially Expressed Genes in Non-functioning Pituitary Adenoma by Transcriptome Analysis. [PDF]

In Vivo
Uzkul ND, Akkoyunlu DS, Cabuk B, Isik EB, Iskenderoglu ET, Keskin SE, Cine N, Savli H.   +7 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Genomic Signatures of Selection Are Enriched in Differentially Expressed Genes in Sticklebacks Adapting to Contrasting Environments. [PDF]

Genome Biol Evol
Kwakye A, Dzikowski N, Wund MA, Veeramah KR.   +3 more
europepmc   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Differentially expressed genes upon MC3465 treatment.

Naive and MTB-infected macrophages derived from 4 individual donors were treated with MC3465 (10 μM) for 4 h and 24 h. Differentially expressed genes were identified by mRNAseq.
Alexandre Giraud-Gatineau (6266753), Alexandre Pawlik (2897897), Francesco Fiorentino (8736915), Wafa Frigui (86861), Olivier Neyrolles (72204), Matthew J. G. Eldridge (11859346), David Hardy (848279), Claude Gutierrez (8119457), Camille Keck (18462503), Nathalie Aulner (369942), Antonello Mai (205768), Saideep Gona (17652438), Luis B. Barreiro (11816886), Alexandra Maure (8841152), Roland Brosch (86868), Anne Danckaert (134213), Emeline Lawarée (18462500), Dante Rotili (462382), Priscille Brodin (57030), Ludovic Tailleux (72195), Mélanie Hamon (18462506)   +20 more
core   +1 more source

Transcriptomic profiling for identifying differentially expressed genes in aneurysm. [PDF]

Bioinformation
Lois GS, Murugan S, Jaganathan MS, S DK, J JB.   +4 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Gut-microbiome composition, single nucleotide polymorphisms, and differentially expressed genes correlate with aggression in an ant. [PDF]

iScience
Krapf P, Cicconardi F, Schilling M, Aigner GP, Klammsteiner T, Ayasse M, Arthofer W, Mikheyev AS, Steiner FM, Schlick-Steiner BC.   +9 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source