Results 131 to 140 of about 23,186 (181)
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Nature, 1958
THE enzymatic reduction of dihydrofolic acid (FH2) to tetrahydrofolic acid (FH4) has been reported by several investigators1–3. Futterman1 noted that dihydrofolic acid was reduced by chicken liver extracts when either reduced di- or tri-phospho-pyridine nucleotide served as the co-factor. We have found that the rate of reduction of dihydrofolic acid by
J M, PETERS, D M, GREENBERG
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THE enzymatic reduction of dihydrofolic acid (FH2) to tetrahydrofolic acid (FH4) has been reported by several investigators1–3. Futterman1 noted that dihydrofolic acid was reduced by chicken liver extracts when either reduced di- or tri-phospho-pyridine nucleotide served as the co-factor. We have found that the rate of reduction of dihydrofolic acid by
J M, PETERS, D M, GREENBERG
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Dismutation of dihydrofolate by dihydrofolate reductase
Biochemistry, 1984Degradation of 7,8-dihydrofolate (H2folate) in the presence of dihydrofolate reductase (DHFR) has been shown due not to an oxygenase activity of the reductase as previously reported but to dismutation of H2folate to folate and 5,6,7,8-tetrahydrofolate (H4folate). The reaction can be followed spectrophotometrically or by analysis of the reaction mixture
R L, Blakley, L, Cocco
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How Dihydrofolate Reductase Facilitates Protonation of Dihydrofolate
Journal of the American Chemical Society, 2003Dihydrofolate Reductase (DHFR) catalyzes the reduction of dihydrofolate (H2F) to tetrahydrofolate. On the basis of 10-12.5 ns molecular dynamics simulations of two conformations (closed and occluded) of the ternary DHFR/NADPH/H2F complex from Escherichia coli and a free energy perturbation approach, we have calculated the pKa value for the N5 atom in ...
Thomas H, Rod, Charles L, Brooks
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Life without dihydrofolate reductase FolA
Trends in Microbiology, 2003Reduced folate derivatives participate in numerous reactions of bacterial intermediary metabolism. Consequently, the well-characterized enzyme implicated in the formation of tetrahydrofolate--dihydrofolate reductase FolA--was considered to be essential for bacterial growth.
Myllykallio, H. +3 more
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The mammalian dihydrofolate reductase locus
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990Synthese de resultats relatifs au gene de la dihydrofolate reductase, en particulier: expression et replication, structure de la chromatine et organisation nucleaire, mutation, reparation et recombinaison du gene; role de ce gene dans la division des cellules tumorales et applications en chimiotherapie et genie ...
J L, Hamlin, C, Ma
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Directed Mutagenesis of Dihydrofolate Reductase
Science, 1983Three mutations of the enzyme dihydrofolate reductase were constructed by oligonucleotide-directed mutagenesis of the cloned Escherichia coli gene. The mutations—at residue 27, aspartic acid replaced with asparagine; at residue 39, proline replaced with cysteine; and at residue 95, glycine replaced with alanine—were
Villafranca, Jesus E. +6 more
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Dihydrofolate Reductase Inhibitors
1984While exploring structural variations of anilinopyrimidines with antimalarial activity, a group at Imperial Chemical Industries found that certain biguanides synthesised as ring-opened analogues possessed good activity against Plasmodium gallinaceum infections in chicks (Curd et al. 1945).
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