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Directed Mutagenesis of Dihydrofolate Reductase
Science, 1983Three mutations of the enzyme dihydrofolate reductase were constructed by oligonucleotide-directed mutagenesis of the cloned Escherichia coli gene. The mutations—at residue 27, aspartic acid replaced with asparagine; at residue 39, proline replaced with cysteine; and at residue 95, glycine replaced with alanine—were
Villafranca, Jesus E. +6 more
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Dihydrofolate reductases as targets for inhibitors
Advances in Enzyme Regulation, 1980Abstract Species differences among dihydrofolate reductases were first detected and most strikingly illustrated by the selective binding of small molecule inhibitors. This led on the one hand to practical applications in the chemotherapy of microbial infections, and on the other hand to stimuli for sequence and conformation studies of representative ...
G H, Hitchings, S L, Smith
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The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase)
Histochemistry, 1979The purpose of the present investigation is to report some histochemical and cytospectrophotometric observations providng more objective evidence for the specific activity of the histochemical method for dihydrofolate reductase activity (5.6.7.8-tetrahydrofolate: E.C. 1.5.1.3).
D, Onicescu, V, Atanasiu
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Structure of Dihydrofolate When Bound to Dihydrofolate Reductase
Journal of the American Chemical Society, 1998The Raman spectrum of dihydrofolate (H2folate) complexed with dihydrofolate reductase (DHFR) and NADP+, believed to be an accurate mimic of the productive DHFR/NADPH/H2folate complex involved in the reaction catalyzed by DHFR, contains bands associated with stretch motions of N5C6 of bound substrate.
Hua Deng, Robert Callender
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The interactions of dihydrofolate with M. tuberculosis dihydrofolate reductase
2016 Second Asian Conference on Defence Technology (ACDT), 2016The molecular dynamics (MD) simulation was used to determine the interactions and binding affinities of Mycobacterium tuberculosis dihydrofolate reductase (mtbDHFR) in complex with dihydrofolate (DHF), which is the natural substrate of the enzyme. The MD simulation approach can predict the ternary complexes of mtbDHFR with NADPH and DHF in the present ...
Pimonluck Sittikornpaiboon +2 more
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Inhibitors of Dihydrofolate Reductase
1983Methotrexate (1b), which remains the single most important antifol anticancer agent after 30 years of clinical use, owes its activity to its stoichiometric inhibition of the enzyme dihydrofolate reductase (EC 1. 5. 1. 3.). Methotrexate was introduced as an antileukaemic agent 10 years before dihydrofolate reductase was demonstrated to be its prime ...
B. Roth, E. Bliss, C. R. Beddell
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