Results 131 to 140 of about 23,657 (193)

Synthesis, in vitro biological evaluation and in silico studies of novel pyrrolidine derived thiosemicarbazones as dihydrofolate reductase inhibitors. [PDF]

RSC Adv
Aftab H, Ullah S, Khan A, Al-Rashida M, Islam T, Alshammari A, Albekairi NA, Taslimi P, Al-Harrasi A, Shafiq Z, Alghamdi S.   +10 more
europepmc   +1 more source

Deep mutational scanning of Pneumocystis jirovecii dihydrofolate reductase reveals allosteric mechanism of resistance to an antifolate. [PDF]

PLoS Genet
Rouleau FD, Dubé AK, Gagnon-Arsenault I, Dibyachintan S, Pageau A, Després PC, Lagüe P, Landry CR.   +7 more
europepmc   +1 more source

Synthesis, molecular docking study and biological evaluation of new pyrrole scaffolds as potential antitubercular agents for dual targeting of enoyl ACP reductase and dihydrofolate reductase. [PDF]

PLoS One
Mahnashi MH, Avunoori S, Gopi S, Shaikh IA, Saif A, Bantun F, Faidah HS, Alhadi AA, Alshehri JH, Alharbi AA, S R PK, Joshi SD.   +11 more
europepmc   +1 more source

The Function of the Active Site Glutamate in Human Dihydrofolate Reductase

Pteridines, 1991
Blakley R. L., Appleman J. R., Tsay J. T., Beard W. A., Freisheim J. H., Ayling J. E., Jablonsky M. J.   +6 more
doaj   +1 more source

TaqMan-quantitative PCR assays applied in <i>Neospora caninum</i> knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase <i>drug selectable markers</i>. [PDF]

Front Cell Infect Microbiol
Rico-San Román L, Hänggeli KPA, Hemphill A, Horcajo P, Collantes-Fernández E, Ortega-Mora LM, Boubaker G.   +6 more
europepmc   +1 more source

Some of the next articles are maybe not open access.

Related searches:

Dismutation of dihydrofolate by dihydrofolate reductase

Biochemistry, 1984
Degradation of 7,8-dihydrofolate (H2folate) in the presence of dihydrofolate reductase (DHFR) has been shown due not to an oxygenase activity of the reductase as previously reported but to dismutation of H2folate to folate and 5,6,7,8-tetrahydrofolate (H4folate). The reaction can be followed spectrophotometrically or by analysis of the reaction mixture
R L, Blakley, L, Cocco
openaire   +2 more sources

Dihydrofolic Acid Reductase

Nature, 1958
THE enzymatic reduction of dihydrofolic acid (FH2) to tetrahydrofolic acid (FH4) has been reported by several investigators1–3. Futterman1 noted that dihydrofolic acid was reduced by chicken liver extracts when either reduced di- or tri-phospho-pyridine nucleotide served as the co-factor. We have found that the rate of reduction of dihydrofolic acid by
J M, PETERS, D M, GREENBERG
openaire   +2 more sources

How Dihydrofolate Reductase Facilitates Protonation of Dihydrofolate

Journal of the American Chemical Society, 2003
Dihydrofolate Reductase (DHFR) catalyzes the reduction of dihydrofolate (H2F) to tetrahydrofolate. On the basis of 10-12.5 ns molecular dynamics simulations of two conformations (closed and occluded) of the ternary DHFR/NADPH/H2F complex from Escherichia coli and a free energy perturbation approach, we have calculated the pKa value for the N5 atom in ...
Thomas H, Rod, Charles L, Brooks
openaire   +2 more sources

Dihydrofolate reductase as a therapeutic target

The FASEB Journal, 1990
The folate antagonists are an important class of therapeutic compounds, as evidenced by their use as antiinfective, antineoplastic, and antiinflammatory drugs. Thus far, all of the clinically useful drugs of this class have been inhibitors of dihydrofolate reductase (DHFR), a key enzyme in the synthesis of thymidylate, and ...
B I, Schweitzer, A P, Dicker, J R, Bertino   +2 more
openaire   +2 more sources

A fluorometric assay for dihydrofolate reductase

Analytical Biochemistry, 1977
Abstract A recording fluorometric assay for dihydrofolate reductase is described. The technique measures the appearance of product, tetrahydrofolate, as the reaction proceeds. The assay can be accomplished with as little as 2 pmol of enzyme and is sufficiently sensitive to allow accurate kinetic studies using very low concentrations of substrate ...
C A, Lindquist, E C, Cadman, J R, Bertino   +2 more
openaire   +2 more sources