Results 81 to 90 of about 197,285 (307)

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Noncovalent Dimerization of Ubiquitin [PDF]

Angewandte Chemie International Edition, 2011
Another kind of dynamics: Ubiquitin noncovalently dimerizes with a dissociation constant of approximately 5 mM. The two subunits adopt an array of relative orientations, utilizing an interface also for binding to other proteins (see picture). Quaternary fluctuation among members of the dimer ensemble constitutes a different kind of dynamics that ...
Liu, Zhu, Zhang, Wei-Ping, Xing, Qiong, Ren, Xuefeng, Liu, Maili, Tang, Chun   +5 more
openaire   +2 more sources

Arginine methylation as a regulatory ratchet in cancer: From substrate selection to malignant‐state stabilization

Molecular Oncology, EarlyView.
Arginine methylation can be viewed as a persistence‐prone post‐translational modification regulated by a network of PRMTs. Competitive and compensatory interactions among PRMTs can redistribute methylation across substrate pools shaped by sequence, structural, spatial, and environmental layers, reinforcing RNA‐processing, chromatin, and signaling ...
So Hyun Kwon, Ji Min Lee
wiley   +1 more source

Mechanisms and therapeutic opportunities of the ribotoxic stress response in cancer

Molecular Oncology, EarlyView.
Cancer cells' high translational demand creates opportunities to therapeutically target ribosome function. Ribosome stalling and collisions activate ZAKα and the ribotoxic stress response (RSR), which can trigger rapid, p53‐independent apoptosis in cancer.
Anastassiya Kim, Maryam Aslam, Elouise Torruella, Alphons Sony, Asif Rahman, Simira Smith, Columba de la Parra, Moira Sauane   +7 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

The Dimerization of Precocene I [PDF]

Natural Product Communications, 2017
In an earlier work we reported that treatment of precocene I with Brönsted and Lewis acids produces its oligomerization, giving dimers, trimers, tetramers, etc. Now, in this article we show that bromination of precocene I with phenyltrimethylammonium tribromide (PTT) blocks its oligomerization giving a dibromo-dimer, which was reduced with tri- n-butyl
Fraga, Braulio M., Cabrera, Inmaculada
openaire   +3 more sources

Inhibiting EGFR dimerization using triazolyl-bridged dimerization arm mimics.

, 2015
The epidermal growth factor receptor (EGFR) is overexpressed in multiple carcinomas and is the focus of a variety of targeted therapies. Here we report the design of peptide-based compounds that mimic the EGFR dimerization arm and inhibit allosteric ...
Laura E. Hanold (705810), Eileen J. Kennedy (374107), Eileen J Kennedy, Laura E Hanold, Natarajan Kannan, Aaron M Beedle, Krishnadev Oruganty (471060), Norman T. Ton (705811), Aaron M. Beedle (563313), Natarajan Kannan (41300), Norman T Ton, Krishnadev Oruganty   +11 more
core   +1 more source

Conserved determinants of lentiviral genome dimerization [PDF]

, 2015
Background Retroviruses selectively package two copies of their unspliced genomes by what appears to be a dimerization-dependent RNA packaging mechanism. Dimerization of human immunodeficiency virus Type-1 (HIV-1) genomes is initiated by “
Yuanyuan Liu, Monti, Sarah, Tran, Thao, Hernandez, Mateo, Yang, Ae L, Thao Tran, Liu, Yuanyuan, Zaki, Jessica, Jennifer Bohn, Er Vega, Venkateswaran Ramakrishnan, Ae Lim Yang, Michael F. Summers, Singh, Rashmi, Summers, Michael F, Ramakrishnan, Venkateswaran, Jan Marchant, Marchant, Jan, Sarah Monti, Michelle Seu, Bohn, Jennifer, Vega, Alexander, Seu, Michelle, Jessica Zaki, Mateo Hern, Rashmi Singh   +25 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Dimer‐Specific FokT‐seq Reveals DNA‐Binding Dimerization and Novel Genomic Targets of TDP‐43

Advanced Science
In postmortem brain tissues of patients with sporadic amyotrophic lateral sclerosis (ALS), the dimerization ability of TAR DNA‐binding protein 43 (TDP‐43) is impaired, accompanied by an accumulation of insoluble TDP‐43.
Mingming Yang, Qi Wang, Ruolan Yan, Xiaochuan Wang, Jianlan Gu   +4 more
doaj   +1 more source