Results 1 to 10 of about 1,137,563 (252)

"Pseudo-Beijing": evidence for convergent evolution in the direct repeat region of Mycobacterium tuberculosis.

PLoS ONE, 2011
BackgroundMycobacterium tuberculosis has a global population structure consisting of six main phylogenetic lineages associated with specific geographic regions and human populations. One particular M.
Lukas Fenner, Bijaya Malla, Béatrice Ninet, Olivier Dubuis, David Stucki, Sonia Borrell, Thembela Huna, Thomas Bodmer, Matthias Egger, Sebastien Gagneux   +9 more
doaj   +3 more sources

Suppression of Huntington’s Disease Somatic Instability by Transcriptional Repression and Direct CAG Repeat Binding [PDF]

Nature Communications
Huntington’s disease arises from a CAG expansion in the huntingtin gene beyond a critical threshold. Current therapeutics primarily aim to reduce toxicity by lowering levels of mutant HTT mRNA and protein.
Ella W. Mathews, Sydney R. Coffey, Annette Gärtner, Jillian Belgrad, Robert M. Bragg, Daniel O’Reilly, Jeffrey P. Cantle, Cassandra McHugh, Ashley Summers, Joachim Fentz, Tom Schwagarus, Antje Cornelius, Ioannis Lingos, Zoe Burch, Marina Kovalenko, Marissa A. Andrew, C. Frank Bennett, Holly B. Kordasiewicz, Deanna M. Marchionini, Hilary Wilkinson, Thomas F. Vogt, Paolo Beuzer, Ricardo M. Pinto, Anastasia Khvorova, David Howland, Vanessa C. Wheeler, Jeffrey B. Carroll   +26 more
doaj   +2 more sources

5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]

Cells
Despite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh, Heather M. Terron, Luke A. Burgard, Dylan D. Butler, Frank M. LaFerla, Shelley Lane, Malcolm A. Leissring   +6 more
doaj   +4 more sources

Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes. [PDF]

PLoS ONE, 2017
The need for generating precisely designed mutations is common in genetics, biochemistry, and molecular biology. Here, I describe a new λ Red recombineering method (Direct and Inverted Repeat stimulated excision; DIRex) for fast and easy generation of ...
Joakim Näsvall
doaj   +3 more sources

Studies on ultrasound-mediated insertion-deletion polymorphisms of DNA and underlying mechanisms based on Ames tester strains

Ultrasonics Sonochemistry, 2023
Low-lethality ultrasound technology has received more and more attention in regulating microorganisms of fermentation industry. Herein, two representative Ames tester strains TA97a and TA98 as model organisms were used to explore the effects of ...
Yunliang Li, Siyu Ruan, Feng Lu, Pengfei Xie, Xiaoshuang Liu, Haile Ma   +5 more
doaj   +1 more source

Accurate contact-based modelling of repeat proteins predicts the structure of new repeats protein families.

PLoS Computational Biology, 2021
Repeat proteins are abundant in eukaryotic proteomes. They are involved in many eukaryotic specific functions, including signalling. For many of these proteins, the structure is not known, as they are difficult to crystallise.
Claudio Bassot, Arne Elofsson
doaj   +1 more source

A Genome-Wide Screen for Genes Affecting Spontaneous Direct-Repeat Recombination in Saccharomyces cerevisiae

G3: Genes, Genomes, Genetics, 2020
Homologous recombination is an important mechanism for genome integrity maintenance, and several homologous recombination genes are mutated in various cancers and cancer-prone syndromes.
Daniele Novarina, Ridhdhi Desai, Jessica A. Vaisica, Jiongwen Ou, Mohammed Bellaoui, Grant W. Brown, Michael Chang   +6 more
doaj   +1 more source

Repeat Diagnoses of Bethesda Category III Thyroid Nodules: What To Do Next? [PDF]

PLoS ONE, 2015
To assess the malignancy rates of thyroid nodules repeatedly classified as Bethesda category III on fine needle aspiration (FNA), and to suggest management guidelines for these lesions.This is a retrospective study that included 395 thyroid nodules ...
Mi Ri Yoo, Hye Mi Gweon, Ah Young Park, Kyung Eun Cho, Jeong-Ah Kim, Ji Hyun Youk, Eun Ju Son   +6 more
doaj   +1 more source

CAG Repeat Instability in the Peripheral and Central Nervous System of Transgenic Huntington’s Disease Monkeys

Biomedicines, 2022
Huntington’s Disease (HD) is an autosomal dominant disease that results in severe neurodegeneration with no cure. HD is caused by the expanded CAG trinucleotide repeat (TNR) on the Huntingtin gene (HTT). Although the somatic and germline expansion of the
In K. Cho, Faye Clever, Gordon Hong, Anthony W. S. Chan   +3 more
doaj   +1 more source

CrisprVi: a software for visualizing and analyzing CRISPR sequences of prokaryotes

BMC Bioinformatics, 2022
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and their spacers are important components of prokaryotic CRISPR-Cas systems.
Lei Sun, Jinbiao Wang, Fu Yan, Gongming Wang, Yun Li, Jinlin Huang   +5 more
doaj   +1 more source