Recombineering in Streptococcus mutans Using Direct Repeat-Mediated Cloning-Independent Markerless Mutagenesis (DR-CIMM) [PDF]
Frontiers in Cellular and Infection Microbiology, 2017 Studies of the dental caries pathogen Streptococcus mutans have benefitted tremendously from its sophisticated genetic system. As part of our own efforts to further improve upon the S. mutans genetic toolbox, we previously reported the development of the
Shan Zhang +5 more
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A Genome-Wide Screen for Genes Affecting Spontaneous Direct-Repeat Recombination in Saccharomyces cerevisiae [PDF]
G3: Genes, Genomes, Genetics, 2020 Homologous recombination is an important mechanism for genome integrity maintenance, and several homologous recombination genes are mutated in various cancers and cancer-prone syndromes.
Daniele Novarina +6 more
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5′ DREDGE: Direct Repeat-Enabled Downregulation of Gene Expression via the 5′ UTR of Target Genes [PDF]
CellsDespite the availability of numerous methods for controlling gene expression, there remains a strong need for technologies that maximize two key properties: selectivity and reversibility.
Sagar J. Parikh +6 more
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CRISPRdigger: detecting CRISPRs with better direct repeat annotations. [PDF]
Sci Rep, 2016 AbstractClustered regularly interspaced short palindromic repeats (CRISPRs) are important genetic elements in many bacterial and archaeal genomes, and play a key role in prokaryote immune systems’ fight against invasive foreign elements. The CRISPR system has also been engineered to facilitate target gene editing in eukaryotic genomes. Using the common
Ge R +6 more
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Epstein-Barr virus DNA. X. Direct repeat within the internal direct repeat of Epstein-Barr virus DNA [PDF]
Journal of Virology, 1981 The 3,360-base-pair internal direct repeat (IR) in Epstein-Barr virus DNA separates the short and long unique DNA domains. IR has a single BamHI site. The juncture between the short unique domain and IR has been mapped by restriction endonucleases and is less than 2,600 nucleotides before the BamHI site in IR.
A, Cheung, E, Kieff
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Role of direct repeat and stem-loop motifs in mtDNA deletions: cause or coincidence? [PDF]
PLoS ONE, 2012 Deletion mutations within mitochondrial DNA (mtDNA) have been implicated in degenerative and aging related conditions, such as sarcopenia and neuro-degeneration. While the precise molecular mechanism of deletion formation in mtDNA is still not completely
Lakshmi Narayanan Lakshmanan +3 more
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Engineering the Direct Repeat Sequence of crRNA for Optimization of FnCpf1-Mediated Genome Editing in Human Cells. [PDF]
Mol Ther, 2018 Lin L +15 more
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"Pseudo-Beijing": evidence for convergent evolution in the direct repeat region of Mycobacterium tuberculosis. [PDF]
PLoS ONE, 2011 BackgroundMycobacterium tuberculosis has a global population structure consisting of six main phylogenetic lineages associated with specific geographic regions and human populations. One particular M.
Lukas Fenner +9 more
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DNA repair pathway-related proteins are involved in the circularization step of microDNA eccDNAfib-L [PDF]
Communications BiologyA large number of extrachromosomal circular DNAs (eccDNAs) are found in eukaryotic cells, but the mechanism behind the formation of eccDNAs remains unknown. EccDNAs smaller than 1000 bp are commonly referred to as microDNAs.
Xinyu Tong +9 more
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Direct and Inverted Repeat stimulated excision (DIRex): Simple, single-step, and scar-free mutagenesis of bacterial genes. [PDF]
PLoS ONE, 2017 The need for generating precisely designed mutations is common in genetics, biochemistry, and molecular biology. Here, I describe a new λ Red recombineering method (Direct and Inverted Repeat stimulated excision; DIRex) for fast and easy generation of ...
Joakim Näsvall
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