Results 51 to 60 of about 1,137,563 (252)

A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal [PDF]

, 2013
Embryonic stem (ES) cell self-renewal efficiency is determined by the Nanog protein level. However, the protein partners of Nanog that function to direct self-renewal are unclear.
Alessia Gagliardi, Ambrosetti DC, Ambrosetti DC, Anastasia Felker, Anastasia I Kousa, Avilion AA, Bailey TL, Bogan AA, Brookes E, Chambers I, Chambers I, Chen X, Chen X, Costa Y, DeLano WL, Dignam JD, Douglas Colby, Engelen E, Favaro R, Festuccia N, Fidalgo M, Florian Halbritter, Gao Z, Genzor CG, Halbritter F, Han JD, Harley VR, Hatano SY, Ho SN, Hooper M, Hutchins AP, Ian Chambers, Jang H, Jason T Weiss, Jauch R, Jepsen K, Jeroen Demmers, Karel Bezstarosti, Kim J, Laemmli UK, Lam CS, Liang J, Ma T, Marson A, Maruyama M, Masui S, McDonel P, Mitsui K, Moretto-Zita M, Mullin NP, Navarro P, Navarro P, Nicholas P Mullin, Noble SM, Pardo M, Parisi S, Perkins DN, Qin J, Raymond A Poot, Rebecca Favaro, Remenyi A, Reynolds N, Rogers MS, Rugg-Gunn PJ, Shi W, Silvia K Nicolis, Simon R Tomlinson, Singh AM, Smith AG, Surface LE, Ura H, van den Berg DL, Wang J, Wang Z, Whyte WA, Wu Q, Yates A, Yuan H, Zappone MV, Zi Ying Tan   +79 more
core   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

DMSO Improves the Ski-Slope Effect in Direct PCR

Applied Sciences, 2021
Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA ...
Joo-Young Kim, Ju Yeon Jung, Da-Hye Kim, Seohyun Moon, Won-Hae Lee, Byung-Won Chun, Dong-Ho Choi   +6 more
doaj   +1 more source

Programmable Sequence-Specific Transcriptional Regulation of Mammalian Genome Using Designer TAL Effectors [PDF]

, 2010
The ability to direct functional proteins to specific DNA sequences is a long-sought goal in the study and engineering of biological processes. Transcription activator–like effectors (TALEs) from Xanthomonas sp.
AJ Bogdanove, B Gonzalez, C Engler, C Engler, F Zhang, Feng Zhang, GE Meister, George M Church, H Scholze, HJ Lee, J Boch, J Boch, Le Cong, LE Rosen, M Christian, MJ Moscou, ML Maeder, N Xu, P Blancafort, P Romer, P Romer, PA Carr, Paola Arlotta, R Morbitzer, RM Gordley, RR Beerli, S Grizot, S Kay, S Kay, Simona Lodato, Sriram Kosuri, T Li, Z Wei   +32 more
core   +2 more sources

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source

Physical Properties of Cervical Mucus of Repeat Breeder Aceh Cattle

Jurnal Veteriner, 2017
This study aimed to investigate the changes in physical properties of cervical mucus of repeat breeder aceh cattle. Subjects were three normal (K1) and repeat breeder (RB) adult female aceh. K1 were post partus cattle >2 months had successful pregrncy by
Tongku Nizwan Siregar, Iin Agustina, Dian Masyitah, Al Azhar, Dasrul Dasrul, Cut Nila Thasmi, Rusli Sulaiman, Razali Daud   +7 more
doaj   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

FEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwara   +6 more
wiley   +1 more source

Nucleotide repeats in mitochondrial genome determine human lifespan [PDF]

, 2008
Direct nucleotide repeats can facilitate deletions of segments of mitochondrial genome1, leading to a wide range of neuromuscular disorders1,2 as well as aging2,3 in humans.
Georgii Bazykin, Konstantin Popadin
core   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Scapinin, the protein phosphatase 1 binding protein, enhances cell spreading and motility by interacting with the actin cytoskeleton.

PLoS ONE, 2009
Scapinin, also named phactr3, is an actin and protein phosphatase 1 (PP1) binding protein, which is expressed in the adult brain and some tumor cells. At present, the role(s) of scapinin in the brain and tumors are poorly understood.
Junji Sagara, Toshiaki Arata, Shunichiro Taniguchi   +2 more
doaj   +1 more source