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Directed evolution of biocatalysts
Current Opinion in Chemical Biology, 1999Directed evolution is being used increasingly in academic and industrial laboratories to modify and improve important biocatalysts. Significant advances during this period of review include compartmentalization of genes and the in vitro translation apparatus in emulsions, as well as several impressive demonstrations of catalyst improvement.
Arnold, Frances H., Volkov, Alexander A.
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Directed Evolution of Aldolases
2004Publisher Summary This chapter describes the directed evolution of aldolases. The detailed protocols for generating aldolases with new catalytic properties are presented. The gene encoding aldolase can be amplified using the standard polymerase chain reaction (PCR) from a genomic DNA preparation using the Qiagen kit and primers flanking the gene with
Dirk, Franke +2 more
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Emerging Topics in Life Sciences, 2020
There are near-to-infinite combinations of possibilities for evolution to happen within nature, making it yet impossible to predict how it occurs. However, science is now able to understand the mechanisms underpinning the evolution of biological systems and can use this knowledge to experimentally mimic nature.
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There are near-to-infinite combinations of possibilities for evolution to happen within nature, making it yet impossible to predict how it occurs. However, science is now able to understand the mechanisms underpinning the evolution of biological systems and can use this knowledge to experimentally mimic nature.
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Journal of Applied Philosophy, 1996
Though we humans are immensely more gifted than other animals, yet we are not the outcome of an inevitable selection of the ‘fittest’. Nor on the other hand is our importance diminished by our evolutionary inheritance. Besides, we are already here! Faith in inevitable progress is a ‘scientific’, not a Christian, delusion.
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Though we humans are immensely more gifted than other animals, yet we are not the outcome of an inevitable selection of the ‘fittest’. Nor on the other hand is our importance diminished by our evolutionary inheritance. Besides, we are already here! Faith in inevitable progress is a ‘scientific’, not a Christian, delusion.
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Angewandte Chemie International Edition, 1998
On the way to a combinatorial biotechnology? The directed evolution of enzymes promises a rapid access to effective biocatalysts. New molecular biology techniques for random mutagenesis in combination with high-throughput screening might revolutionize the creation of enzymes with new and improved properties.
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On the way to a combinatorial biotechnology? The directed evolution of enzymes promises a rapid access to effective biocatalysts. New molecular biology techniques for random mutagenesis in combination with high-throughput screening might revolutionize the creation of enzymes with new and improved properties.
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Directed evolution of novel polymerases
Biomolecular Engineering, 2005DNA and RNA polymerases evolved to function in specific environments with specific substrates to propagate genetic information in all living organisms. The commercial availability of these polymerases has revolutionized the biotechnology industry, but for many applications native polymerases are limited by their stability or substrate recognition. Thus,
Rebecca C, Holmberg +2 more
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Protein Design by Directed Evolution
Annual Review of Biophysics, 2008While nature evolved polypeptides over billions of years, protein design by evolutionary mimicry is progressing at a far more rapid pace. The mutation, selection, and amplification steps of the evolutionary cycle may be imitated in the laboratory using existing proteins, or molecules created de novo from random sequence space, as starting templates ...
Christian, Jäckel +2 more
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Nature Methods, 2011
Phage-assisted continuous evolution of proteins automates and accelerates selection, allowing hundreds of rounds of evolution to occur in a single week.
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Phage-assisted continuous evolution of proteins automates and accelerates selection, allowing hundreds of rounds of evolution to occur in a single week.
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Directed Evolution of Adenoviruses
2016The ability to evolve viruses in cell culture in the face of selective pressure is an invaluable method to elucidate the molecular mechanisms of synthetic or natural antivirals, expand tropism, or alter virulence. Recently, mutations to the human adenovirus polymerase that reduce replicative fidelity were described, and we have incorporated one of ...
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