Results 91 to 100 of about 489,553 (296)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution

SynBio
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth.
Vamsi Krishna Gali, Kang Lan Tee, Tuck Seng Wong   +2 more
doaj   +1 more source

Gi- and Gs-coupled GPCRs show different modes of G-protein binding. [PDF]

, 2018
More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered.
Altenbach, Christian, Caro, Lydia N, Dror, Ron O, Ernst, Oliver P, Hollingsworth, Scott A, Hubbell, Wayne L, Latorraca, Naomi R, Van Eps, Ned   +7 more
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

eLife, 2015
Understanding the extent to which enzyme evolution is reversible can shed light on the fundamental relationship between protein sequence, structure, and function.
Miriam Kaltenbach, Colin J Jackson, Eleanor C Campbell, Florian Hollfelder, Nobuhiko Tokuriki   +4 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Protein directed evolution

Revista de Química, 2008
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational
Roberto Laos
doaj  

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Consequences of a covariant Description of Heavy Ion Reactions at intermediate Energies

, 1995
Heavy ion collisions at intermediate energies are studied by using a new RQMD code, which is a covariant generalization of the QMD approach. We show that this new implementation is able to produce the same results in the nonrelativistic limit (i.e. 50MeV/
A. Bonasera, A. Komar, A. Komar, A. Komar, A. P. Balachandran, Amand Faessler, C. Fuchs, C. Grégoire, C. M. Ko, D. Dominici, D. Dominici, D. Dominici, D. G. Currie, E. C. G. Sudarshan, E. Lehmann, E. Lehmann, F. Rohrlich, G. Batko, G. F. Bertsch, G. F. Bertsch, H. Kruse, H. Sorge, I. T. Todorov, J. Aichelin, J. Aichelin, J. Cugnon, J. Samuel, J. Samuel, P. A. M. Dirac, R. Schmidt, Rajeev K. Puri, S. W. Huang, T. Kodama, T. Maruyama, T. Maruyama, W. Bauer, W. Cassing   +36 more
core   +1 more source

Function‐driven design of a surrogate interleukin‐2 receptor ligand

FEBS Letters, EarlyView.
Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang, Zelin Cheng, Teng Li, Fulian Wang, Liangminghui Zhang, Xiuxiu He, Lili Liu, Wei Wang, Aibin Liang, Guang Yang   +9 more
wiley   +1 more source