Results 81 to 90 of about 493,855 (292)

Computer simulations explain mutation-induced effects on the DNA editing by adenine base editors. [PDF]

, 2020
Adenine base editors, which were developed by engineering a transfer RNA adenosine deaminase enzyme (TadA) into a DNA editing enzyme (TadA*), enable precise modification of A:T to G⋮C base pairs. Here, we use molecular dynamics simulations to uncover the
Komor, Alexis C, Paesani, Francesco, Rallapalli, Kartik L   +2 more
core  

Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution [PDF]

, 2000
Enzymes isolated from organisms native to cold environments generally exhibit higher catalytic efficiency at low temperatures and greater thermosensitivity than their mesophilic counterparts.
Arnold, Frances H., Miyazaki, Kentaro, Wintrode, Patrick L.   +2 more
core   +1 more source

A Bibliometric Analysis of Publications in Uremic Toxins From 1991 to 2024

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Uremic toxins are a growing area of research in nephrology, with significant implications in the progression and treatment of chronic kidney disease (CKD) and the management of end‐stage kidney disease (ESKD). This bibliometric analysis aims to evaluate the global research trends, key contributors, and the impact of publications in ...
Yuh‐Shan Ho, Karim M. Soliman, Wisit Cheungpasitporn, Wannasit Wathanavasin, Ahmed Daoud, Ahmed I. Kamal, Balázs Szamosfalvi, Tibor Fülöp   +7 more
wiley   +1 more source

Engineering and In Vitro Selection of a Novel AAV3B Variant with High Hepatocyte Tropism and Reduced Seroreactivity

Molecular Therapy: Methods & Clinical Development, 2020
Limitations to successful gene therapy with adeno-associated virus (AAV) can comprise pre-existing neutralizing antibodies to the vector capsid that can block cellular entry, or inefficient transduction of target cells that can lead to sub-optimal ...
Moanaro Biswas, Damien Marsic, Ning Li, Chenhui Zou, Gloria Gonzalez-Aseguinolaza, Irene Zolotukhin, Sandeep R.P. Kumar, Jyoti Rana, John S.S. Butterfield, Oleksandr Kondratov, Ype P. de Jong, Roland W. Herzog, Sergei Zolotukhin   +12 more
doaj   +1 more source

Structural and dynamic changes associated with beneficial engineered single-amino-acid deletion mutations in enhanced green fluorescent protein. [PDF]

, 2014
Single-amino-acid deletions are a common part of the natural evolutionary landscape but are rarely sampled during protein engineering owing to limited and prejudiced molecular understanding of mutations that shorten the protein backbone.
Arpino, JA, Jones, DD, Rizkallah, PJ
core   +3 more sources

Revealing the structure of land plant photosystem II: the journey from negative‐stain EM to cryo‐EM

FEBS Letters, EarlyView.
Advances in cryo‐EM have revealed the detailed structure of Photosystem II, a key protein complex driving photosynthesis. This review traces the journey from early low‐resolution images to high‐resolution models, highlighting how these discoveries deepen our understanding of light harvesting and energy conversion in plants.
Roman Kouřil
wiley   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Crafting Genetic Diversity: Unlocking the Potential of Protein Evolution

SynBio
Genetic diversity is the foundation of evolutionary resilience, adaptive potential, and the flourishing vitality of living organisms, serving as the cornerstone for robust ecosystems and the continuous evolution of life on Earth.
Vamsi Krishna Gali, Kang Lan Tee, Tuck Seng Wong   +2 more
doaj   +1 more source

Feedback and Feeding in the Context of Galaxy Evolution withSPICA: Direct Characterisation of Molecular Outflows and Inflows [PDF]

Publications of the Astronomical Society of Australia, 2017
AbstractA far-infrared observatory such as theSPace Infrared telescope for Cosmology and Astrophysics, with its unprecedented spectroscopic sensitivity, would unveil the role of feedback in galaxy evolution during the last ~10 Gyr of the Universe (z= 1.5–2), through the use of far- and mid-infrared molecular and ionic fine structure lines that trace ...
E. González-Alfonso, L. Armus, F. J. Carrera, V. Charmandaris, A. Efstathiou, E. Egami, J. A. Fernández-Ontiveros, J. Fischer, G. L. Granato, C. Gruppioni, E. Hatziminaoglou, M. Imanishi, N. Isobe, H. Kaneda, D. Koziel-Wierzbowska, M. A. Malkan, J. Martín-Pintado, S. Mateos, H. Matsuhara, G. Miniutti, T. Nakagawa, F. Pozzi, F. Rico-Villas, G. Rodighiero, P. Roelfsema, L. Spinoglio, H. W. W. Spoon, E. Sturm, F. van der Tak, C. Vignali, L. Wang   +30 more
openaire   +9 more sources

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source