Results 101 to 110 of about 493,855 (292)

Improving Hydrolytic Activity and Enantioselectivity of Epoxide Hydrolase from Phanerochaete chrysosporium by Directed Evolution

Molecules
Epoxide hydrolases (EHs) catalyze the conversion of epoxides into vicinal diols. The epoxide hydrolase gene from P. chrysosporium was previously cloned and subjected to site-directed mutation to study its enzyme activity, but the results were ...
Huanhuan Shao, Pan Xu, Xiang Tao, Xinyi He, Chunyan Pu, Shaorong Liang, Yingxin Shi, Xiaoyan Wang, Hong Feng, Bin Yong   +9 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Complex networks theory for analyzing metabolic networks

, 2006
One of the main tasks of post-genomic informatics is to systematically investigate all molecules and their interactions within a living cell so as to understand how these molecules and the interactions between them relate to the function of the organism,
A. B. Horne, A. Bairoch, A. Broder, A. L. Barabasi, A. L. Barabasi, A. Samal, A. Wagner, B. M. Bakker, C. H. Schilling, C. Wagner, D. J. Watts, E. Ravasz, H. Jeong, H. Kitano, H. Lipson, H. W. Ma, H. W. Ma, H. W. Ma, Hong Yu, J. A. Papin, J. C. M. Mombach, J. Gagneur, J. Stelling, J. Stelling, Jianhua Luo, Jing Zhao, L. H. Hartwell, M. Arita, M. C. Palumbo, M. Faloutsos, M. Kanehisa, M. Kanehisa, M. Nakao, M. V. Martinov, N. Lemke, P. D. Karp, P. D. Karp, P. Erdös, P. Holme, R. Albert, R. Guimera, R. Mahadevan, R. Overbeek, R. Schuster, S. Goto, S. Schuster, S. Schuster, S. Wuchty, Yixue Li, Z. N. Oltvai, Z. W. Cao   +50 more
core   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

{Co4O4} Cubanes in a conducting polymer matrix as bio-inspired molecular oxygen evolution catalysts

Nature Communications
Exploration of efficient molecular water oxidation catalysts for long-term application remains a key challenge for the conversion of renewable energy sources into fuels.
Shangkun Li, Zeyi Zhang, Walker R. Marks, Xinan Huang, Hang Chen, Dragos C. Stoian, Rolf Erni, Carlos A. Triana, Greta R. Patzke   +8 more
doaj   +1 more source

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Directed Evolution with Fast and Efficient Selection Technologies

CHIMIA, 2001
Directed molecular evolution has proven to be a very powerful concept for the generation of proteins with improved properties, such as increased activity, binding affinity, folding efficiency or enhanced chemical and/or thermodynamic stability ...
Ekkehard Mössner, Andreas Plückthun
doaj  

Improving glyphosate oxidation activity of glycine oxidase from Bacillus cereus by directed evolution.

PLoS ONE, 2013
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative ...
Tao Zhan, Kai Zhang, Yangyan Chen, Yongjun Lin, Gaobing Wu, Lili Zhang, Pei Yao, Zongze Shao, Ziduo Liu   +8 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Rate and cost of adaptation in the Drosophila Genome

, 2014
Recent studies have consistently inferred high rates of adaptive molecular evolution between Drosophila species. At the same time, the Drosophila genome evolves under different rates of recombination, which results in partial genetic linkage between ...
Lässig, Michael, Mustonen, Ville, Schiffels, Stephan   +2 more
core   +1 more source