Results 91 to 100 of about 493,855 (292)

Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

eLife, 2015
Understanding the extent to which enzyme evolution is reversible can shed light on the fundamental relationship between protein sequence, structure, and function.
Miriam Kaltenbach, Colin J Jackson, Eleanor C Campbell, Florian Hollfelder, Nobuhiko Tokuriki   +4 more
doaj   +1 more source

Protostellar Outflow Evolution in Turbulent Environments

, 2008
The link between turbulence in star formatting environments and protostellar jets remains controversial. To explore issues of turbulence and fossil cavities driven by young stellar outflows we present a series of numerical simulations tracking the ...
Adam Frank, Alice C. Quillen, Andrew J. Cunningham, Bally, Bally, Bally, Bally, Banerjee, Blitz, Cabrit, Carroll, Cunningham, Cunningham, Cunningham, Cunningham, Delamarter, Elmegreen, Eric G. Blackman, Hartigan, Heyer, Jonathan Carroll, Knee, Krumholz, Krumholz, Krumholz, Larson, Lebedev, Lee, Li, Mac Low, Mac Low, Matzner, Matzner, Matzner, Nakamura, Quillen, Shepherd, Smith, Stanke, Stone, Sutherland, Warin, Yirak   +42 more
core   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Protein directed evolution

Revista de Química, 2008
Directed evolution allows us to explore protein functionalities not required in the natural environment. It mimics natural genetic processes and selective pressures. This approach is used when the molecular basis is not completely understood and rational
Roberto Laos
doaj  

Transposon-Based Approaches for Generating Novel Molecular Diversity During Directed Evolution [PDF]

, 2014
This chapter introduces a set of transposon-based methods that were developed to sample trinucleotide deletion, trinucleotide replacement, and domain insertion. Each approach has a common initial step that utilizes an engineered version of the Mu transposon called MuDel.
Jones, Darran Dafydd, Arpino, James A. J., Baldwin, Amy Joy, Edmundson, Matthew C.   +3 more
openaire   +3 more sources

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Directed transport as a mechanism for protein folding in vivo

, 2009
We propose a model for protein folding in vivo based on a Brownian-ratchet mechanism in the multidimensional energy landscape space. The device is able to produce directed transport taking advantage of the assumed intrinsic asymmetric properties of the ...
Alberts B., Bhutani N., Ellis R. J., Ernesto González-Candela, Kramer G., Lapanje S., Levinthal C., van Kampen N. G., Visscher K., Víctor Romero-Rochín   +9 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source