Results 261 to 270 of about 495,896 (276)
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Directional mutation pressure, mutator mutations, and dynamics of molecular evolution
Journal of Molecular Evolution, 1993Using a general form of the directional mutation theory, this paper analyzes the effect of mutations in mutator genes on the G+C content of DNA, the frequency of substitution mutations, and evolutionary changes (cumulative mutations) under various degrees of selective constraints.
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A novel biotinylated suicide inhibitor for directed molecular evolution of lipolytic enzymes
Bioorganic & Medicinal Chemistry, 2000A bifunctional activity label (8) for directed molecular evolution of lipolytic enzymes has been designed and synthesized. The structure is composed of a 4-nitrophenyl activated phosphonate, that is, a suicide substrate of lipases/esterases, connected to a biotin moiety through a spacer containing a disulfide bridge. The phosphonate (3) was prepared by
H J, Deussen +3 more
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Directed Molecular Evolution: Bridging the Gap between Genomics Leads and Commercial Products
OMICS: A Journal of Integrative Biology, 2002Agricultural crops, engineered to express transgenic traits, have been rapidly adopted by farmers since the initial commercialization of this technology in 1996. However, despite nearly 20 years of research in agricultural biotechnology, only two product categories have achieved commercial success: plants containing ...
Michael W, Lassner, David, McElroy
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Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs
Nature Protocols, 2010G protein-coupled receptors (GPCRs) and their downstream signaling cascades contribute to most physiological processes and a variety of human diseases. Isolating the effects of GPCR activation in an in vivo experimental setting is challenging as exogenous ligands have off-target effects and endogenous ligands constantly modulate the activity of native ...
Shuyun, Dong +2 more
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Directed molecular evolution by machine learning and the influence of nonlinear interactions
Journal of Theoretical Biology, 2005Alternative search strategies for the directed evolution of proteins are presented and compared with each other. In particular, two different machine learning strategies based on partial least-squares regression are developed: the first contains only linear terms that represent a given residue's independent contribution to fitness, the second contains ...
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Directed molecular evolution of nitrite oxido-reductase by DNA-shuffling.
Biomedical and environmental sciences : BES, 2007To develop directly molecular evolution of nitrite oxido-reductase using DNA-shuffling technique because nitrobacteria grow extremely slow and are unable to nitrify effectively inorganic nitrogen in wastewater treatment.The norB gene coding the ndtrite oxido-reductase in nitrobacteria was cloned and sequenced.
Jun-Wen, Li +4 more
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[Bacterial resistance to antibiotics, an exemplary model of directed molecular evolution].
Revue medicale de Liege, 1998The emergence and spread of antibiotic resistance among bacteria make use of the same strategies and obey the same laws as natural evolution but the process is oriented and accelerated.
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Strategies to Develop Allergy Immunotherapeutics with Applications of Directed Molecular Evolution
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents, 2003Erik Karrer +3 more
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