Results 71 to 80 of about 26,459 (307)

Protein Stabilization by Introduction of Cross-Strand Disulfides^†

, 2016
Disulfides cross-link residues in a protein that are separated in primary sequence and stabilize the protein through entropic destabilization of the unfolded state.
Kausik Chakraborty (184226), C. Ramakrishnan (2256529), Raghavan Varadarajan (76468), Sudhir Thakurela (398994), P. Shaik Syed Ali (411027), Ravindra Singh Prajapati (411026), S. Indu (2477002)   +6 more
core   +1 more source

Interleukin-2 signalling is modulated by a labile disulfide bond in the CD132 chain of its receptor [PDF]

Open Biology, 2012
Certain disulfide bonds present in leucocyte membrane proteins are labile and can be reduced in inflammation. This can cause structural changes that result in downstream functional effects, for example, in integrin activation.
Clive Metcalfe, Peter Cresswell, A. Neil Barclay   +2 more
doaj   +1 more source

Topical ocular application of aggrelyte-2A reduces lens stiffness in mice

Frontiers in Ophthalmology, 2023
Presbyopia is the progressive loss of the ability of the lens to focus on nearby objects due to its increased stiffness. It occurs in the mid-40s and continues to worsen until the mid-60s.
Sudipta Panja, Mi-Hyun Nam, Hanmant Gaikwad, Johanna Rankenberg, Ram H. Nagaraj, Ram H. Nagaraj   +5 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Measurement of Mixed Disulfides Including Glutathionylated Proteins

, 2010
Mixed disulfides between protein cysteines and low-molecular-weight thiol cysteine or glutathione lead to the formation of cysteinylated proteins or glutathionylated proteins.
Raffaella Priora, SALZANO S., Lucia Coppo (2512243), Pietro Ghezzi, CHERUBINI DI SIMPLICIO P., Raffaella Priora (16047182), Sonia Salzano (740669), COPPO L., Lucia Coppo, Paolo Di Simplicio, Pietro Ghezzi (4462387), Paolo Di Simplicio (16047185), GHEZZI P., Sonia Salzano, PRIORA R.   +14 more
core   +3 more sources

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Disulfides by reduction of thiosulfonic s-esters

, 1982
Disulfides are smoothly prepared from thiosulfonic S-esters by chlorotrimethylsilane and sodium iodide. In addition thiosulfonic S-esters have been shown to be probable intermediates in other already known reactions leading to ...
G. PALUMBO, PALUMBO, GIOVANNI, Michelangelo Parrilli, PARRILLI, MICHELANGELO, Orsola Neri, Carla Ferreri, C. FERRERI, Giovanni Palumbo, CAPUTO, ROMUALDO, R. CAPUTO, Romualdo Caputo, O. NERI, FERRERI, CARLA   +12 more
core   +1 more source

Chemoselective synthesis of diaryl disulfides via a visible light-mediated coupling of arenediazonium tetrafluoroborates and CS2

Beilstein Journal of Organic Chemistry, 2017
A highly efficient and chemoselective method for the synthesis of diaryl disulfides is developed via a visible light-promoted coupling of readily accessible arenediazonium tetrafluoroborates and CS2.
Jing Leng, Shi-Meng Wang, Hua-Li Qin
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source