Terminal restriction fragment length polymorphism is an “old school” reliable technique for swift microbial community screening in anaerobic digestion [PDF]
The microbial community in anaerobic digestion has been analysed through microbial fingerprinting techniques, such as terminal restriction fragment length polymorphism (TRFLP), for decades.
De Vrieze, Jo +3 more
core +6 more sources
Cold adaptation and replicable microbial community development during long-term low temperature anaerobic digestion treatment of synthetic sewage [PDF]
The development and, activity of a cold-adapting microbial community was monitored during low temperature anaerobic digestion (LtAD) treatment of wastewater.
Cysneiros, D. +6 more
core +2 more sources
Bacterial and Archaeal DNA from Lake Sediments
This book, entitled Tracking Environmental Change Using Lake Sediments: Volume 6 – Sedimentary DNA, provides an overview of the applications of sedimentary DNA-based approaches to paleolimnological studies. These approaches have shown considerable potential in providing information about the long-term changes of overall biodiversity in lakes and their ...
Vuillemin, A. +4 more
openaire +2 more sources
Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation [PDF]
We discovered a thermostable enzyme from the archaeon Pyrococcus furiosus ( Pfu ), which increases yields of PCR product amplified with Pfu DNA polymerase. A high molecular mass (>250 kDa) complex with PCR-enhancing activity was purified from Pfu
Holly H, Hogrefe +3 more
openaire +2 more sources
Solution structure of an archaeal DNA binding protein with an eukaryotic zinc finger fold. [PDF]
While the basal transcription machinery in archaea is eukaryal-like, transcription factors in archaea and their viruses are usually related to bacterial transcription factors.
Florence Guillière +7 more
doaj +1 more source
Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle [PDF]
The Rowett Institute of Nutrition and Health and SRUC are funded by the Rural and Environment Science and Analytical Services Division (RESAS) of the Scottish Government.
Duthie, Carol-Anne +9 more
core +3 more sources
The Epigenomic Landscape of Prokaryotes. [PDF]
DNA methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. Studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and ...
Matthew J Blow +18 more
doaj +1 more source
Archaeal RNA polymerase arrests transcription at DNA lesions [PDF]
Transcription elongation is not uniform and transcription is often hindered by protein-bound factors or DNA lesions that limit translocation and impair catalysis. Despite the high degree of sequence and structural homology of the multi-subunit RNA polymerases (RNAP), substantial differences in response to DNA lesions have been reported.
Alexandra M, Gehring +1 more
openaire +2 more sources
Photoreactivation of DNA by an Archaeal nucleoprotein Sso7d [PDF]
Sso7d is a chromosomal protein of hyperthermophilic Archaea. The crystal structure of Sso7d–d(GTAAT I UAC) 2 has been clarified at high resolution, showing that the protein binds in the minor groove of DNA, causing a sharp kink of ≈60°.
Ryu, Tashiro +2 more
openaire +2 more sources
Culture-independent 16S rRNA gene analysis approach was used to explore and evaluate archaea in a polluted site, El-Zeitia, Suez Gulf, Egypt. Metagenomic DNA was extracted from a sediment sample.
Hosam Easa Elsaied
doaj +1 more source

