Results 71 to 80 of about 762,695 (253)

Deletion mutants of bacteriophage phiX174 [PDF]

open access: yes, 1972
Mutants of bacteriophage phiX174 have been isolated that are less dense than wild-type phiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded
Benbow, Robert M.   +2 more
core  

A cellulose-based bioassay for the colorimetric detection of pathogen DNA [PDF]

open access: yes, 2014
Cellulose-paper-based colorimetric bioassays may be used at the point of sampling without sophisticated equipment. This study reports the development of a colorimetric bioassay based on cellulose that can detect pathogen DNA.
Goyal, Madhu   +3 more
core   +1 more source

Deciphering of interactions between platinated DNA and HMGB1 by hydrogen/deuterium exchange mass spectrometry [PDF]

open access: yes, 2017
A high mobility group box 1 (HMGB1) protein has been reported to recognize both 1,2-intrastrand crosslinked DNA by cisplatin (1,2-cis-Pt-DNA) and monofunctional platinated DNA using trans-[PtCl2(NH3)(thiazole)] (1-trans-PtTz-DNA).
Du, Zhifeng   +9 more
core   +2 more sources

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

open access: yesFEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp   +5 more
wiley   +1 more source

The Escherichia coli RutR transcription factor binds at targets within genes as well as intergenic regions. [PDF]

open access: yes, 2008
The Escherichia coli RutR protein is the master regulator of genes involved in pyrimidine catabolism. Here we have used chromatin immunoprecipitation in combination with DNA microarrays to measure the binding of RutR across the chromosome of ...
Akira Ishihama   +24 more
core   +5 more sources

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

open access: yesInternational Journal of Analytical Chemistry, 2020
Trans-Zeatin is the major active phytohormone in immature corn kernels. Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-zeatin was constructed.
Ping Sun, Xiwei Zhang, Xianxiang Wang
doaj   +1 more source

Parallel DNA Synthesis : Two PCR product from one DNA template [PDF]

open access: yesarXiv, 2013
Conventionally in a PCR reaction, Primers binds to DNA template in an antiparallel manner and template DNA is amplified as it is. Here we describe an approach in which First primer binds in a complementary parallel orientation leading to synthesis in parallel direction.
arxiv  

Brucella NyxA and NyxB dimerization enhances effector function during infection

open access: yesFEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre   +4 more
wiley   +1 more source

Hierarchical coassembly of DNA–triptycene hybrid molecular building blocks and zinc protoporphyrin IX

open access: yesBeilstein Journal of Nanotechnology, 2016
Herein, we describe the successful construction of composite DNA nanostructures by the self-assembly of complementary symmetrical 2,6,14-triptycenetripropiolic acid (TPA)–DNA building blocks and zinc protoporphyrin IX (Zn PpIX).
Rina Kumari   +6 more
doaj   +1 more source

RecA filament sliding on DNA facilitates homology search

open access: yeseLife, 2012
During homologous recombination, RecA forms a helical filament on a single stranded (ss) DNA that searches for a homologous double stranded (ds) DNA and catalyzes the exchange of complementary base pairs to form a new heteroduplex.
Kaushik Ragunathan   +2 more
doaj   +1 more source

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