Results 61 to 70 of about 506,971 (266)

Strain promoted azide alkyne cycloaddition, an efficient surface functionalization strategy for microRNA magnetic separation

Next Materials
In an emergency context, the detection of microRNA (miRNA), which are potential biomarkers of cardiovascular and muscular pathologies is hampered by the time required for the various steps involved: treatment of the patient sample, reverse transcription (
Djamila Kechkeche, Sirine El Mousli, Claire Poujouly, Emilie Secret, Vincent Dupuis, Isabelle Le Potier, Marie-Emmanuelle Goriot, Julien Siracusa, Sébastien Banzet, Jean Gamby, Jean-Michel Siaugue   +10 more
doaj   +1 more source

Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli

mBio, 2016
Recombineering, in vivo genetic engineering with bacteriophage homologous recombination systems, is a powerful technique for making genetic modifications in bacteria.
Lynn C. Thomason, Nina Costantino, Donald L. Court   +2 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Hierarchical coassembly of DNA–triptycene hybrid molecular building blocks and zinc protoporphyrin IX

Beilstein Journal of Nanotechnology, 2016
Herein, we describe the successful construction of composite DNA nanostructures by the self-assembly of complementary symmetrical 2,6,14-triptycenetripropiolic acid (TPA)–DNA building blocks and zinc protoporphyrin IX (Zn PpIX).
Rina Kumari, Sumit Singh, Mohan Monisha, Sourav Bhowmick, Anindya Roy, Neeladri Das, Prolay Das   +6 more
doaj   +1 more source

A Single Complementary-Sense Transcript of a Geminiviral DNA β Satellite Is Determinant of Pathogenicity

Molecular Plant-Microbe Interactions, 2005
Small circular single-stranded DNA satellites, termed DNAβ, have recently been found associated with some geminivirus infections. The DNA β associated with Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan.
Muhammad Saeed, S. A. Akbar Behjatnia, Shahid Mansoor, Yusuf Zafar, Shahida Hasnain, M. Ali Rezaian   +5 more
doaj   +1 more source

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

International Journal of Analytical Chemistry, 2020
Trans-Zeatin is the major active phytohormone in immature corn kernels. Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-zeatin was constructed.
Ping Sun, Xiwei Zhang, Xianxiang Wang
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil

São Paulo Medical Journal, 2002
CONTEXT: Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.
Andréa Carla de Souza Góes, Dayse Aparecida da Silva, Cristiane Santana Domingues, João Marreiro Sobrinho, Elizeu Fagundes de Carvalho   +4 more
doaj   +1 more source

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

FEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui, Agnes Banreti, Armelle L. Ernst, Guillaume Leseigneur, Uwe J. Meierhenrich   +4 more
wiley   +1 more source

Isolation of the Complementary DNA for Human Transcobalamin II

Experimental Biology and Medicine, 1989
A complementary DNA (cDNA) clone coding for transcobalamin II (TCII) has been isolated from a human umbilical vein endothelial cell cDNA library. The cDNA is 1.9 Kb and includes the nucleotide sequence which encodes the NH2-terminal 19 amino acids of human TCII.
O, Platica, R, Geneczko, A, Regec, E V, Quadros, S P, Rothenberg   +4 more
openaire   +2 more sources