Results 51 to 60 of about 44,514 (187)

Structural Insights Into the Function of Leishmania major Adenylosuccinate Lyase

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT One of several intriguing aspects of kinetoplastid biochemistry is the complete dependence on host purines and purine recycling due to the lack of a de novo purine biosynthesis pathway. Adenylosuccinate lyase (ASL, EC 4.3.2.2) is a key enzyme in the purine synthesis pathway responsible for the conversion of adenylosuccinate into adenosine ...
Ivan R. e Silva, Monique Mantovani, Maria E. S. D. Lino, Aline O. Albuquerque, João H. M. da Silva, Ronaldo A. P. Nagem, Adriana L. Rojas, Geraldo R. Sartori, Eduardo Horjales, David Campbell, Otavio H. Thiemann   +10 more
wiley   +1 more source

A chromosome-scale genome assembly of Giardia duodenalis by long-read sequencing of ten trophozoites

Scientific Data
Giardia duodenalis, a protozoan parasite of important zoonotic concern, is estimated to cause approximately 280 million human infections annually worldwide. Currently, genome sequencing of G.
Jingjing Sun, Yuancai Chen, Yizhuo Wei, Kaihui Zhang, Yin Fu, Zi Yan, Xingyu Zhu, Sumei Zhang, Longxian Zhang, Junqiang Li   +9 more
doaj   +1 more source

A rapid and simple method of detection of Blepharisma japonicum using PCR and immobilisation on FTA paper

BMC Ecology, 2003
Background The rapid expansion in the availability of genome and DNA sequence information has opened up new possibilities for the development of methods for detecting free-living protozoa in environmental samples.
Hughes Jacqueline M, Hide Geoff, McNuff Robert   +2 more
doaj   +1 more source

DNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR

The Korean Journal of Parasitology, 2014
PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles is therefore clear. QIAamp® DNA Stool Mini Kit (Qiagen) was evaluated for its ability to recover DNA from oocysts/cysts directly from feces. Twenty-five Giardia-positive,
openaire   +2 more sources

Response of Fecal Bacteria and Fungi to Tannin‐Rich Diets in Sika Deer (Cervus nippon): Evidence from Both Feeding Experiments and Field Investigations

Integrative Zoology, EarlyView.
Feeding with tannin‐rich diets altered the fecal microbial composition and increased the relative abundance of tannin‐degrading microbes. We hypothesize that fecal bacteria and fungi may play important roles in helping herbivores adapt to tannin‐rich diets but respond to different tannin concentrations varies.
Di Zhu, Feng Wu, Peiying Wen, Haiyang Gao, Xiaoran Hao, Hongfang Wang   +5 more
wiley   +1 more source

Formation of linear amplicons with inverted duplications in Leishmania requires the MRE11 nuclease. [PDF]

PLoS Genetics, 2014
Extrachromosomal DNA amplification is frequent in the protozoan parasite Leishmania selected for drug resistance. The extrachromosomal amplified DNA is either circular or linear, and is formed at the level of direct or inverted homologous repeated ...
Marie-Claude N Laffitte, Marie-Michelle Genois, Angana Mukherjee, Danielle Légaré, Jean-Yves Masson, Marc Ouellette   +5 more
doaj   +1 more source

Domination Versus Sisterhoods in the Blood Microbiota of Migrating Birds: Patterns of Within‐ and Between‐Individual Blood Parasite Diversity Revealed Through Metabarcoding

Integrative Zoology, EarlyView.
Avian haemosporidian blood parasites are typically identified through Sanger sequencing of a partial cytochrome b fragment, the MalAvi barcoding region. Next‐generation sequencing is seldom used for avian blood parasite identification; this study demonstrates a higher detection rate of co‐infections via metabarcoding and its possible implications ...
Peter Pibaque, Gaia Porporato, Simone Cescutti, Alanis Cruz‐Flores, Tobias Busche, Anika Winker, Tim Maximilian Rapp, Patrick Bergkamp, Anna Doneva, Nayden Chakarov   +9 more
wiley   +1 more source

Drivers of Host–Pathogen Community Assemblages in European Forests and Urban Green Spaces

Integrative Zoology, EarlyView.
ABSTRACT Despite advances in understanding infectious diseases, the persistence and re‐emergence of wildlife pathogens continue to raise public and veterinary health concerns. This study investigates the relationship between biodiversity and rodent‐borne diseases in Europe, focusing on habitat alterations and their impact on rodent diversity.
Vincent Sluydts, Marie Bouilloud, Maxime Galan, Hussein Alburkat, Anaïs Bordes, Vincent Bourret, Valeria Colombo, Luc DeBruyn, Lara Dutra, Jana Eccard, Jasmin Firozpoor, Romain Gallet, Maciej Grzybek, Heikki Henttonen, Jens Jacob, Andrew McManus, Tarja Sironen, Peter Stuart, Caroline Tatard, Benjamin Roche, Herwig Leirs, Nathalie Charbonnel   +21 more
wiley   +1 more source

DNA Topoisomerase as Drug Target in unicellular protozoan parasite Leishmania donovani

INDIAN JOURNAL OF PHYSIOLOGY AND ALLIED SCIENCES, 2022
Visceral leishmaniasis or Kala-Azar is a neglected tropical disease caused by the unicellular kinetoplastid parasite Leishmania donovani.The treatments available have various shortcomings like toxicity and high cost. The development of resistance in the strains makes thetreatment less effective. Hence, there is a need for new and better treatments. DNA
openaire   +1 more source

β- d -Glucosyl-hydroxymethyluracil is a conserved DNA modification in kinetoplastid protozoans and is abundant in their telomeres [PDF]

Proceedings of the National Academy of Sciences, 1998
The unusual DNA base β- d -glucosyl-hydroxymethyluracil, called “J,” replaces ≈0.5–1% of Thy in DNA of African trypanosomes but has not been found in other organisms thus far. In Trypanosoma brucei , J is located predominantly in repetitive DNA, and its
Van Leeuwen, F, Taylor, MC, Mondragon, A, Moreau, H, Gibson, WC, Kieft, R, Borst, P   +6 more
openaire   +3 more sources