Results 261 to 270 of about 145,655 (292)

Transcription of tomato ribosomal DNA and the organization of the intergenic spacer

Molecular and General Genetics MGG, 1990
The organization of the intergenic spacer of a 9.04 kb tomato ribosomal RNA gene (rDNA) was determined. The 3258 bp spacer contains two major repeat elements enclosing a region which includes 351 bp of an 81.8% A --T rich sequence. A block of nine 53 bp repeats begins 388 bp downstream from the 3' end of the 25S rRNA.
K L, Perry, P, Palukaitis
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Nucleotide sequence of the intergenic spacer (IGS) of the tomato ribosomal DNA

Plant Molecular Biology, 1989
The intergenic spacer (IGS) of the ribosomal DNA spans the region from the 3' end of the 25S rRNA gene to the 5' end of the 18S rRNA gene. Here we report the sequence of the IGS of tomato (Lycopersicon esculentum)
W, Schmidt-Puchta, I, Günther, H L, Sänger   +2 more
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Identification of variability of ribosomal DNA spacer from Pseudomonas soil isolates

Canadian Journal of Microbiology, 1994
The polymerase chain reaction was used to amplify the spacer region located between the 16S and 23S ribosomal RNA genes of strains of Pseudomonas fluorescens and Pseudomonas putida isolated from peat bog, canola field, or arctic plants. Some of these amplified spacer regions were used to probe Southern blots of total DNA digests from the various ...
S, Gill, J, Belles-Isles, G, Brown, S, Gagné, C, Lemieux, J P, Mercier, P, Dion   +6 more
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Functional analysis of internal transcribed spacer 2 of Saccharomyces cerevisiae ribosomal DNA

Journal of Molecular Biology, 1992
Using the previously described "tagged ribosome" (pORCS) system for in vivo mutational analysis of yeast rDNA, we show that small deletions in the 5'-terminal portion of ITS2 completely block maturation of 26 S rRNA at the level of the 29 SB precursor (5.8 S rRNA-ITS2-26 S rRNA). Various deletions in the 3'-terminal part, although severely reducing the
C A, van der Sande, M, Kwa, R W, van Nues, H, van Heerikhuizen, H A, Raué, R J, Planta   +5 more
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Tobacco ribosomal DNA spacer element stimulates amplification and expression of heterologous genes

Nature Biotechnology, 2000
Here we show that the cis-acting genetic element aps (amplification-promoting sequence), isolated from the nontranscribed spacer region of tobacco ribosomal DNA (rDNA), increases the level of expression of recombinant proteins. Transgenic tobacco plants, transformed with expression cassettes containing the herbicide-resistant acetolactate synthase (hr ...
N, Borisjuk, L, Borisjuk, S, Komarnytsky, S, Timeva, V, Hemleben, Y, Gleba, I, Raskin   +6 more
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Genotyping of Trichophyton rubrum by analysis of ribosomal-DNA intergenic spacer regions

Mycopathologia, 2007
An attempt was made to explore the genotyping of Trichophyton rubrum (T. rubrum) and the relationship between genotype and geographical origin using ribosomal restriction endonuclease polymorphic analysis. The total DNA was extracted by cetyltrimethyl ammonium bromide (CTAB). The probe was amplified from part of the 18S, ITSI, 5.8S, and ITSII region of
Guoling, Yang, Lijia, An, Qiao, Li, Jingrong, Lin, Weida, Liu, Liji, Jin, Xiran, Lin   +6 more
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Fourteen internal transcribed spacers in the circular ribosomal DNA of Euglena gracilis

Journal of Molecular Biology, 1990
Cytoplasmic ribosomes from Euglena gracilis contain 16 rRNA components. These include the typical 5 S, 5.8 S and 19 S rRNAs that are found in other eukaryotes as well as 13 discrete small RNAs that interact to form the equivalent of eukaryotic 25-28 S rRNA (accompanying paper).
M N, Schnare, J R, Cook, M W, Gray
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Typing truffle species by PCR amplification of the ribosomal DNA spacers

Mycological Research, 1994
Variation within the internal transcribed spacer (ITS) and the intergenic spacer (IGS) of the ribosomal RNA genes of European species of Tuber was examined by polymerase chain reaction (PCR) and coupled restriction fragment length polymorphism (RFLP) analysis.
Henrion, B., Chevalier, G., Martin, F.
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Polyploidy and Ribosomal-DNA Spacer Variability in Poa labillardieri

Australian Journal of Botany, 1991
With changing agricultural practices, poa tussock (Poa labillardieri Steud.) has become a serious weed of Australian tableland pastures. To determine whether there are any genetic differences within the species that might have a bearing on this problem, accessions of P.
CE May, MH Campbell
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Is higher-order structure conserved in eukaryotic ribosomal DNA intergenic spacers?

Journal of Molecular Evolution, 1992
Computer-based structural analysis of the ribosomal DNA intergenic spacer (IGS) from the mosquito Aedes albopictus revealed a potential to form strong and extensive secondary structures throughout a 4.7-kilobase (kb) region. The predicted stability of secondary structures was particularly high within a 3.15-kb region containing 17 tandem 201 base-pair ...
G D, Baldridge, M W, Dalton, A M, Fallon
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