Results 71 to 80 of about 69,429 (309)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Two Distinct, Geographically Overlapping Lineages of the Corallimorpharian Ricordea Florida (Cnidaria: Hexacorallia: Ricordeidae) [PDF]

, 2011
We examined the genetic variation of the corallimorpharian Ricordea florida; it is distributed throughout the Caribbean region and is heavily harvested for the marine aquarium trade. Eighty-four distinct individuals of R. florida were sequenced from four
Lado-Insua, T., Rhyne, Andrew L., Rodríguez-Matos, L., Schizas, N. V., Torres-Pratts, H.   +4 more
core   +1 more source

Swelling‐Programmed Topographical Guidance for Dynamic Spheroid Self‐Assembly via a Mechanochemical Hydrogel Niche

Advanced Functional Materials, EarlyView.
A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam, Seon Yeong Chae, Young Woo Kwon, Hye Jin Heo, Hye Rin Lee, Ji Hyeok Lee, Dong‐Wook Han, Yun Hak Kim, Suck Won Hong   +8 more
wiley   +1 more source

Morphological and DNA-based Classification of Cyathus sp. Isolates from Thailand (Basidiomycota, Nidulariaceae)

Journal of Pure and Applied Microbiology, 2020
Cyathus sp. isolates from three areas in Thailand (Khon Kaen University, Nam Nao National Park and Phu Ruea, Loei Province) were morphologically characterized by their peridiocarp, peridioles and basidiospores.
Waraporn Sutthisa, Niwat Sanoamuang
doaj   +1 more source

Variability of Human rDNA

Cells, 2021
In human cells, ribosomal DNA (rDNA) is arranged in ten clusters of multiple tandem repeats. Each repeat is usually described as consisting of two parts: the 13 kb long ribosomal part, containing three genes coding for 18S, 5.8S and 28S RNAs of the ...
Evgeny Smirnov, Nikola Chmúrčiaková, František Liška, Pavla Bažantová, Dušan Cmarko   +4 more
doaj   +1 more source

The domestication of the probiotic bacterium Lactobacillus acidophilus [PDF]

, 2014
Lactobacillus acidophilus is a Gram-positive lactic acid bacterium that has had widespread historical use in the dairy industry and more recently as a probiotic. Although L.
Aerts, Maarten, Bray, James E, Bull, Matthew J, Jolley, Keith A, Mahenthiralingam, Eshwar, Maiden, Martin CJ, Marchesi, Julian R, Vandamme, Peter   +7 more
core   +3 more sources

Infusible Extracellular Matrix Biomaterial Enhances Cell‐Specific Pro‐Repair Responses Following Acute Myocardial Infarction

Advanced Healthcare Materials, EarlyView.
We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin, Alexander Chen, Quincy P. Lyons, Michael B. Nguyen, Maria L. Karkanitsa, Justin Yu, Van K. Ninh, Emily Gardner, Elyse G. Wong, Sriya N. Paleti, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Colin G. Luo, Zhenxing Fu, Kevin R. King, Karen L. Christman   +18 more
wiley   +1 more source

Multiple ITS Copies Reveal Extensive Hybridization within Rheum (Polygonaceae), a Genus That Has Undergone Rapid Radiation [PDF]

, 2014
During adaptive radiation events, characters can arise multiple times due to parallel evolution, but transfer of traits through hybridization provides an alternative explanation for the same character appearing in apparently non-sister lineages.
A Gelman, A Keller, A Kovarik, A Kovarik, Ailan Wang, AL Wang, AR Griffin, AS Losina-Losinskaya, BB Liu, BG Baldwin, BG Baldwin, C Hughes, C Kiefer, C Koetschan, C Lexer, CL Schoch, D Harpke, D Harpke, D Harpke, D Schluter, DN Young, Dongshi Wan, DS Wan, DV Jobes, DY Zhang, DZ Li, ER Glor, ES Buckler, ES Buckler, F Herder, F Ma, F Ronquist, G Benson, G Besnard, G Bänfer, G Muir, G Sergey, GE McKinnon, GL Stebbins, GP Robideau, GW Grimm, I Alvarez, J Lihova, J Schultz, J Song, JD Thompson, JE Richardson, JF Manen, JJ Doyle, JQ Liu, JQ Liu, JQ Liu, Jun Wang, JW Ochieng, K Mao, K Tamura, KC Rowe, KJ Parsons, LH Rieseberg, LQ Xiao, LY Wang, M Arakaki, M Mayol, M Ryberg, M Wolf, MA Buchheim, MA Koch, MH Yang, NH Barton, O Seehausen, P Poczai, PR Grant, R Jaretzky, R Matyasek, RC Albertson, RH Nilsson, RI Milne, Richard Milne, RR Liu, RW Masueli, S Chen, S Dumolin-Lape'gue, S Gavrilets, S Guindon, S Hartmann, S Joly, S Shaw, Shilin Chen, T Denk, T Sang, TA Hall, TC Chin, TC Kao, TJ Givnish, TM Hardig, TM Porter, TT Xu, UK Schliewen, Xiaotao Bai, Xu Zhang, XY Zheng, Y Kita, YJ Wang, Yongshuai Sun, YP Hu, YS Sun   +105 more
core   +3 more sources

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

A new disease of Erica arborea in Italy caused by Neofusicoccum luteum

Phytopathologia Mediterranea, 2015
Shoot blight was observed on Erica arborea L., in a natural growing area on Caprera Island (Italy), during 2011 and 2012. Fungal isolates obtained from 324 symptomatic shoots were identified as Neofusicoccum luteum by analysis of morphological and ...
Benedetto LINALDEDDU, Bruno SCANU, Salvatore SEDDAIU, Antonio DEIDDA, Lucia MADDAU, Antonio FRANCESCHINI   +5 more
doaj   +1 more source