Results 251 to 260 of about 578,618 (302)
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Thermophoresis of single stranded DNA
ELECTROPHORESIS, 2010AbstractThe manipulation and analysis of biomolecules in native bulk solution is highly desired; however, few methods are available. In thermophoresis, the thermal analog to electrophoresis, molecules are moved along a microscopic temperature gradient. Its theoretical foundation is still under debate, but practical applications for analytics in biology
Philipp, Reineck +2 more
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Tight Single-Stranded DNA Knots
Journal of Biomolecular Structure and Dynamics, 1993Trefoil (3(1)) and figure-8 (4(1)) knots have been synthesized from DNA molecules containing two single-turn helical domains, linked by four oligodeoxythymidine linkers. Both topologies are derived from the same DNA molecule. The tightest knots are fashioned by minimizing the lengths of the linkers.
H, Wang, S M, Du, N C, Seeman
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Isolation of Single‐Stranded DNA
Current Protocols in Molecular Biology, 2014AbstractSingle‐stranded DNA (ssDNA) is often used for DNA sequencing as well as microarray and hybridization technologies. Asymmetric PCR or exonuclease digestion followed by urea gel separation and isolation on streptavidin‐coated magnetic beads are commonly used for this purpose. These two methods may not yield large amounts of highly purified ssDNA.
Yuji, Wakimoto +2 more
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The Single-Stranded DNA Phages
CRC Critical Reviews in Microbiology, 1975(1975). The Single-Stranded DNA Phages. CRC Critical Reviews in Microbiology: Vol. 4, No. 2, pp. 161-223.
David Tilton Denhardt, Peter Model
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Scientific American, 1962
The molecule of the hereditary material deoxyribonucleic acid (DNA) normally consists of two interwoven strands. The discovery of a form with only one strand promises to clarify how the molecule replicates.
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The molecule of the hereditary material deoxyribonucleic acid (DNA) normally consists of two interwoven strands. The discovery of a form with only one strand promises to clarify how the molecule replicates.
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Single-Stranded DNA Family Shuffling
2004Publisher Summary This chapter examines the various aspects of single-stranded DNA family shuffling. DNA shuffling is a technique that allows accelerated and directed protein evolution in vitro . This technique has been shown to be powerful in combining independently isolated mutations of a gene into a single progeny. By using the sense strand ssDNA
Osamu, Kagami +2 more
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Molecular Biotechnology, 1996
DNA sequence and expression analyses have greatly benefited from using M13 and pUC derived cloning vectors and their polycloning sites. A chronology of the original concepts and experiments is reviewed.
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DNA sequence and expression analyses have greatly benefited from using M13 and pUC derived cloning vectors and their polycloning sites. A chronology of the original concepts and experiments is reviewed.
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Single-Stranded DNA Phage Origins
1988Since the rediscovery of bacteriophage o X174 in the 1950 s by Robert Sinsheimer (1959), the single-stranded DNA phages have been widely used as model systems in molecular biology. o X174 can be considered as their “ godfather. ” The single-stranded DNA bacteriophages are divided into two classes based on the morphology of their representatives, either
P D, Baas, H S, Jansz
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Stretching single stranded DNA
Soft Matter, 2011Recently, single molecule force spectroscopy experiments provided much important information about the elastic properties of a single stranded DNA. Theoretical efforts, which followed these experiments could not provide satisfactory explanation for some of the observed results.
Sanjay Kumar, Garima Mishra
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Single-stranded DNA damage: Protecting the single-stranded DNA from chemical attack
DNA Repair, 2020Cellular processes, such as DNA replication, recombination and transcription, require DNA strands separation and single-stranded DNA is formation. The single-stranded DNA is promptly wrapped by human single-stranded DNA binding proteins, replication protein A (RPA) complex.
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