The yeast Rad7/Rad16/Abf1 complex generates superhelical torsion in DNA that is required for nucleotide excision repair [PDF]
, 2004 Nucleotide excision repair (NER) in eukaryotes removes DNA base damage as an oligonucleotide in a complex series of reactions. The nature of the dual incision reactions on either side of the damaged base has been extensively investigated.
Owen-Hughes, Tom +4 more
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Role of DNA superhelicity in partitioning of the pSC101 plasmid
Cell, 1990 Previous work has shown that a cis-acting locus (termed par for partitioning) on the pSC101 plasmid accomplishes its stable inheritance in dividing cell populations. We report here that the DNA of pSC101 derivatives lacking the par region shows a decrease in overall superhelical density as compared with DNA of wild-type pSC101.
C A, Miller, S L, Beaucage, S N, Cohen
openaire +2 more sources
Multiple structures of RNA polymerase II isolated from human nuclei by ChIP-CryoEM analysis
Nature CommunicationsRNA polymerase II (RNAPII) is a central transcription enzyme that exists as multiple forms with or without accessory factors, and transcribes the genomic DNA packaged in chromatin.
Tomoya Kujirai +12 more
doaj +1 more source
A Superhelical Spiral in the Escherichia coli DNA Gyrase a C-terminal Domain Imparts Unidirectional Supercoiling Bias [PDF]
, 2019 DNA gyrase is unique among type II topoisomerases in that its DNA supercoiling activity is unidirectional. The C-terminal domain of the gyrase A subunit (GyrA-CTD) is required for this supercoiling bias.
Verdine, Gregory L. +3 more
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DNA superhelical density and CRP-mediated lac promoter activation [PDF]
, 1991 The cyclic AMP receptor protein (CRP) functions in Escherichia coli in the regulation of several catabolite-sensitive operons. Cyclic AMP (cAMP) forms a complex with CRP which binds to specific DNA sites near the promoter of lactose operon {lacP) and ...
Lee, Hae Kyung
core
Conformational changes induced by salt in complexes of histones and superhelical nuclear DNA. [PDF]
, 1981 When HeLa cells are lysed in solutions containing a non-ionic detergent and 0.75 M-NaCl, structures are released that retain many of the morphological features of nuclei.
Cook, Peter, Cook, PR, Levin, JM
core
Free energy profiles for unwrapping the outer superhelical turn of nucleosomal DNA under torsional stress [PDF]
, 2019 Torsional stress gives a significant impact on the structure and dynamics of chromatin. For example, the torsion stress generates supercoiling in chromatin, where negatively supercoiled domains form a decompacted large-scale chromatin structure and are ...
Kono, Hidetoshi +5 more
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Caracterização das DNA topoisomerases II de Trypanosoma rangeli [PDF]
, 2012 Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Biotecnologia, Florianópolis, 2010DNA topoisomerases são enzimas que participam de diversos processos celulares tais como: replicação ...
Stoco, Patrícia Hermes
core
Initiation of Agrobacterium tumefaciens T-DNA processing: purified proteins VirD1 and VirD2 catalyze site- and strand-specific cleavage of superhelical T-border DNA in vitro [PDF]
, 1995 T-DNA processing during agroinfection of plants is initiated by site- and strand-specific incision at the T-DNA border sequences of the Ti plasmid. Two proteins are required for this reaction: VirD2 (49.6 kDa), catalyzing a site-specific cleaving-joining
Scheiffele, Peter +2 more
core
Synthesis of Superhelical Simian Virus 40 Deoxyribonucleic Acid in Cell Lysates* [PDF]
In vivo-labeled SV40 replicating DNA molecules can be converted into covalently closed superhelical SV40 DNA (SV40(I) using a lysate of sv40-infected monkey cells containing intact nuclei.
Berg, P., DePamphilis, M. L., Beard, P.
core +1 more source