Results 101 to 110 of about 7,352 (214)
PLoS Genetics, 2012 To investigate the role of DNA topoisomerases in transcription, we have studied global gene expression in Saccharomyces cerevisiae cells deficient for topoisomerases I and II and performed single-gene analyses to support our findings.
Jakob Madsen Pedersen +9 more
doaj +1 more source
Ultraviolet light irradiation of PM2 superhelical DNA
Nucleic Acids Research, 1977 Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2.
M, Woodworth-Gutai +3 more
openaire +3 more sources
, 1993 Initiation of bacteriophage φ29 DNA replication is activated by protein p6, a viral double-stranded DNA-binding protein that forms a nucleoprotein complex at the viral replication origins.
Serrano, Manuel +3 more
core +1 more source
Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila Embryos [PDF]
, 1992 We descrtbe a cell·free system, derlved (rom preblastoderm DrosophÜIJ embryos. ror tbe efficient assembly of eloned DNA into chromatin. Tbe cbromatin assembly system ulilizes cndogcDous eure histones aod assembly racton aod yields loog arrays of ...
Earl Wu +3 more
core
Transcription of polyoma virus DNA after interaction with nuclear proteins in vitro
, 1976 RNA polymerase B, histones and non-histone chromosomal proteins (NHP) extracted from calf thymus nuclei were used for transcription experiments with superhelical form 1 and linear Eco R1 cleaved Polyoma virus DNA.
G. Meneguzzi +2 more
core +1 more source
Dye titrations of covalently closed supercoiled DNA analyzed by agarose gel electrophoresis [PDF]
, 1976 We have developed a rapid electrophoretic technique for performing ethidium bromide dye titrations in cylindrical 0.7% agarose gels. The technique was used to analyze the extent of supercoiling in circular covalently closed SV40, Co1E1, and pSC101 DNA ...
DeLeys, Robert J., Jackson, David A.
core +1 more source
, 2015 Type IB topoisomerases unwind positive and negative DNA supercoils and play a key role in removing supercoils that would otherwise accumulate at replication and transcription forks.
Breeana G. Anderson (1325925) +1 more
core +1 more source
, 1995 A Monte Carlo model for the generation of superhelical DNA structures at thermodynamic equilibrium (Klenin et al., 1991; Vologodskii et al., 1992) was modified to account for the presence of local curvature.
Klenin, K.V. +2 more
core +1 more source
Selective binding of specific mouse genomic DNA fragments by mouse vimentin filaments in vitro
, 1996 Mouse vimentin intermediate filaments (IFs) reconstituted in vitro were analyzed for their capacity to select certain DNA sequences from a mixture of about 500−bp−long fragments of total mouse genomic DNA.
Shoeman, R. +7 more
core +1 more source
A pH-dependent structural transition in the homopurine-homopyrimidine tract in superhelical DNA
, 1986 С помощью двумерного электрофореза обнаружен структурный переход нового типа в гомопурин-гомопиримидиновом участке в сверхспиральной ДНК. В отличие от других исследованных переходов, этот переход сильно зависит от рН, что проявляется в уменьшении ...
Миркин, С.М. +2 more
core +1 more source