Results 81 to 90 of about 11,225 (206)

The Superhelical Density of Nuclear DNA from Human Cells [PDF]

European Journal of Biochemistry, 1977
Structures resembling nuclei may be released by gently lysing human cells in solutions containing non‐ionic detergents and high concentrations of salt. These structures, which we call nucleoids, sediment in sucrose gradients containing the intercalating agent, actinomycin D, in the manner characteristic of superhelical DNA.
Cook, P, Brazell, I
openaire   +3 more sources

The Concise Guide to PHARMACOLOGY 2025/26: Ion channels

British Journal of Pharmacology, Volume 182, Issue S1, Page S152-S241, December 2025.
The Concise Guide to Pharmacology 2025/26 marks the seventh edition in this series of biennial publications in the British Journal of Pharmacology. Presented in landscape format, the guide provides a comparative overview of the pharmacology of drug target families. The concise nature of the Concise Guide refers to the style of presentation, being clear,
Stephen P. H. Alexander, Jörg Striessnig, Alasdair J. Gibb, Alistair A. Mathie, Emma L. Veale, Eamonn Kelly, Chloe J. Peach, Jane F. Armstrong, Elena Faccenda, Simon D. Harding, Christopher Southan, Jamie A. Davies, Richard W. Aldrich, Bernard Attali, Austin M. Baggetta, Elvir Becirovic, David J. Beech, Martin Biel, Roslyn M. Bill, Ana I. Caceres, William A. Catterall, Han Chow Chua, Alex C. Conner, Henry Danahay, Paul Davies, Katrien De Clerq, Markus Delling, Francesco Di Virgilio, Simonetta Falzoni, Wei Fang, Stefanie Fenske, Anna Fortuny‐Gomez, Samuel Fountain, George K. Chandy, Mohammad‐Reza Ghovanloo, Steve A. N. Goldstein, Martin Gosling, Christian Grimm, Stephan Grissmer, Kotdaji Ha, Tim G. Hales, Verena Hammelmann, Israel Hanukoglu, Meiqin Hu, Ad P. Ijzerman, Sairam V. Jabba, Mike Jarvis, Anders A. Jensen, Sven E. Jordt, Leonard K. Kaczmarek, Stephan Kellenberger, Charles Kennedy, Brian King, Philip Kitchen, Qiang Liu, Joseph W. Lynch, Rian W. Manville, Jessica Meades, Verena Mehlfeld, Annette Nicke, Stefan Offermanns, Edward Pérez‐Reyes, Leigh D. Plant, Stephan A. Pless, Lachlan D. Rash, Dejian Ren, Hussein N. Rubaiy, Mootaz M. Salman, Werner Sieghart, Lucia G. Sivilotti, Trevor G. Smart, Terrance P. Snutch, Paolo Tammaro, Jinbin Tian, James S. Trimmer, Charlotte Van den Eynde, Paola Vergani, Joris Vriens, Stephen G. Waxman, Aguan D. Wei, Brenda T. Winn, Heike Wulff, Haoxing Xu, Fan Yang, Lixia Yue, Xiaoli Zhang, Michael Zhu   +86 more
wiley   +1 more source

Ultraviolet light irradiation of PM2 superhelical DNA

Nucleic Acids Research, 1977
Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2.
M, Woodworth-Gutai, J, Lebowitz, A C, Kato, D T, Denhardt   +3 more
openaire   +3 more sources

Transferability and Reproducibility of the HepaRG CometChip Assay

Environmental and Molecular Mutagenesis, Volume 66, Issue 9, Page 478-491, November 2025.
ABSTRACT This interlaboratory evaluation of HepaRG CometChip was conducted to assess transferability and reproducibility of this new approach methodology (NAM) across four laboratories. Concentrations inducing up to ~70% relative cytotoxicity were determined by the organizing laboratory, and frozen chemical formulation blocks were sent to each ...
Leslie Recio, Carol Swartz, Lincoln Martin, Emily Rottinger, Stefan Pfuhler, Erica Pinkus, Daniel J. Roberts, Leon F. Stankowski, Simran Kaushal, Norah A. Owiti, Bevin P. Engelward   +10 more
wiley   +1 more source

Biological Consequences of Tightly Bent DNA: The Other Life of a Macromolecular Celebrity [PDF]

, 2006
The mechanical properties of DNA play a critical role in many biological functions. For example, DNA packing in viruses involves confining the viral genome in a volume (the viral capsid) with dimensions that are comparable to the DNA persistence length ...
Garcia, Hernan G., Grayson, Paul, Han, Lin, Inamdar, Mandar, Kondev, Jane, Nelson, Philip C., Phillips, Rob, Widom, Jonathan, Wiggins, Paul A.   +8 more
core   +3 more sources

Interaction of f1 histone with superhelical DNA. [PDF]

Proceedings of the National Academy of Sciences, 1975
The superhelicity of double-stranded, closed circular SV40 DNA was altered by the addition of various amounts of ethidium bromide. The interaction of f1 histone with the series of molecules of various superhelicities was studied. The extent of interaction increases with increasing superhelicity regardless of whether it is of the positive or negative ...
T, Vogel, M F, Singer
openaire   +2 more sources

Extracellular Vesicle‐Mediated Regulation of H3C14 Contributes to Gemcitabine Resistance in Bladder Cancer

Journal of Extracellular Vesicles, Volume 14, Issue 11, November 2025.
H3C14 modulates gemcitabine metabolism and is selectively excluded via extracellular vesicles (EVs) in gemcitabine (GCB)‐resistant bladder cancer. CD9⁺/CD63⁺/CD81⁺ EVs (Transport‐EVs) transmit resistance, whereas CD147⁺/LAMB1⁺ EVs (Excretion‐EVs) excrete tumour‐suppressive cargos, highlighting the functional divergence of EV subtypes in GCB resistance.
Cheng‐Shuo Huang, Dah‐Shyong Yu, Shih Sheng Jiang, Ying‐Si Wu, Jar‐Yi Ho, Cheng‐Ping Yu   +5 more
wiley   +1 more source

Cytotoxicity of psammaplin A from a two-sponge association may correlate with the inhibition of DNA replication

BMC Cancer, 2004
Background SV40 DNA replication system is a very useful tool to understand the mechanism of replication, which is a tightly regulated process. Many environmental and cellular factors can induce cell cycle arrest or apoptosis by inhibiting DNA replication.
You Song, Yoon Hyun, Park Jang-Su, Kim Dong-Kyoo, Ryu Seung, Ahn Eun-Young, Jiang Yahong, Lee Burm-Jong, Lee Dong, Jung Jee H   +9 more
doaj   +1 more source

Kinetic Accessibility of Buried DNA Sites in Nucleosomes [PDF]

, 2006
Using a theoretical model for spontaneous partial DNA unwrapping from histones, we study the transient exposure of protein-binding DNA sites within nucleosomes. We focus on the functional dependence of the rates for site exposure and reburial on the site
C. Gardiner, C. H. Wiggins, M. Doi, Richard A. Neher, Ulrich Gerland, Wolfram Möbius   +5 more
core   +2 more sources

The dissociation of nuclear proteins from superhelical DNA

Journal of Cell Science, 1978
ABSTRACT Structures retaining many of the morphological features of nuclei may be released by gently lysing human cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids contain superhelical DNA.
Levin, J, Jost, E, Cook, P
openaire   +3 more sources