DNA Supercoiling: an Ancestral Regulator of Gene Expression in Pathogenic Bacteria? [PDF]
Computational and Structural Biotechnology Journal, 2019 DNA supercoiling acts as a global and ancestral regulator of bacterial gene expression. In this review, we advocate that it plays a pivotal role in host-pathogen interactions by transducing environmental signals to the bacterial chromosome and ...
Shiny Martis B. +4 more
doaj +5 more sources
Investigating DNA supercoiling in eukaryotic genomes [PDF]
Briefings in Functional Genomics, 2017 Supercoiling is a fundamental property of DNA, generated by polymerases and other DNA-binding proteins as a consequence of separating/bending the DNA double helix.
Nick Gilbert
exaly +6 more sources
DNA supercoiling in bacteria: state of play and challenges from a viewpoint of physics based modeling [PDF]
Frontiers in Microbiology, 2023 DNA supercoiling is central to many fundamental processes of living organisms. Its average level along the chromosome and over time reflects the dynamic equilibrium of opposite activities of topoisomerases, which are required to relax mechanical stresses
Ivan Junier +4 more
doaj +4 more sources
DNA supercoiling differences in bacteria result from disparate DNA gyrase activation by polyamines. [PDF]
PLoS Genetics, 2020 DNA supercoiling is essential for all living cells because it controls all processes involving DNA. In bacteria, global DNA supercoiling results from the opposing activities of topoisomerase I, which relaxes DNA, and DNA gyrase, which compacts DNA. These
Alexandre Duprey, Eduardo A Groisman
doaj +3 more sources
The bacterial promoter spacer modulates promoter strength and timing by length, TG-motifs and DNA supercoiling sensitivity [PDF]
Scientific Reports, 2021 Transcription, the first step to gene expression, is a central coordination process in all living matter. Besides a plethora of regulatory mechanisms, the promoter architecture sets the foundation of expression strength, timing and the potential for ...
Carlo A. Klein +3 more
doaj +3 more sources
DNA supercoiling and transcription in bacteria: a two-way street [PDF]
BMC Molecular and Cell Biology, 2019 Background The processes of DNA supercoiling and transcription are interdependent because the movement of a transcription elongation complex simultaneously induces under- and overwinding of the DNA duplex and because the initiation, elongation and ...
Charles J. Dorman
doaj +3 more sources
FEDS: a Novel Fluorescence-Based High-Throughput Method for Measuring DNA Supercoiling
mBio, 2020 DNA supercoiling (DS) is essential for life because it controls critical processes, including transcription, replication, and recombination. Current methods to measure DNA supercoiling in vivo are laborious and unable to examine single cells.
Alexandre Duprey, Eduardo A. Groisman
doaj +2 more sources
The bacterial RNA polymerase-associated CarD protein couples promoter activity to DNA supercoiling [PDF]
Nature CommunicationsRNA polymerase (RNAP) must unwind duplex DNA prior to transcription initiation. In bacteria, unwinding starts at a DNA motif called the promoter −10 element. Specifically, non-template strand bases interact with RNAP to trigger the process.
David Forrest +2 more
doaj +2 more sources
The Transcriptome of Streptococcus pneumoniae Induced by Local and Global Changes in Supercoiling [PDF]
Frontiers in Microbiology, 2017 The bacterial chromosome is compacted in a manner optimal for DNA transactions to occur. The degree of compaction results from the level of DNA-supercoiling and the presence of nucleoid-binding proteins.
Adela G. de la Campa +5 more
doaj +7 more sources
DNA supercoiling-mediated G4/R-loop formation tunes transcription by controlling the access of RNA polymerase [PDF]
Nature CommunicationsRNA polymerase (RNAP) is a processive motor that modulates DNA supercoiling and reshapes DNA structures. The feedback loop between the DNA topology and transcription remains elusive.
Jihee Hwang +8 more
doaj +2 more sources