Results 11 to 20 of about 2,295,017 (227)
DNA supercoiling during transcription
Biophysical Reviews, 2016 The twin-supercoiled-domain model describes how transcription can drive DNA supercoiling, and how DNA supercoiling, in turn plays an important role in regulating gene transcription. In vivo and in vitro experiments have disclosed many details of the complex interactions in this relationship, and recently new insights have been gained with the help of ...
Jie Ma, Michelle D Wang
exaly +5 more sources
DNA supercoiling restricts the transcriptional bursting of neighboring eukaryotic genes
Molecular Cell, 2023 DNA supercoiling has emerged as a major contributor to gene regulation in bacteria. The impact of DNA supercoiling on transcription dynamics in eukaryotes is less clear.
Stefano Coppola +2 more
exaly +3 more sources
HU of Streptococcus pneumoniae Is Essential for the Preservation of DNA Supercoiling [PDF]
Frontiers in Microbiology, 2018 The histone-like protein HU is a conserved nucleoid-associated protein that is involved in the maintenance of the bacterial chromosome architecture. It is the only known nucleoid-associated protein in Streptococcus pneumoniae, but it has not been studied.
María-José Ferrándiz +4 more
doaj +3 more sources
Nonequilibrium dynamics and action at a distance in transcriptionally driven DNA supercoiling [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2021 Significance Positive and negative supercoiling refers to the overwinding or underwinding, respectively, of a DNA double helix. Supercoiling is important and ubiquitous, as all cells, from bacterial to human, deploy proteins to keep their genome ...
Yair Augusto Gutiérrez Fosado +2 more
exaly +4 more sources
mBio, 2016 Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans, but relatively little is known about the global regulation of virulence factors during infection of chickens or humans.
Claire Shortt +5 more
doaj +2 more sources
Structural interplay between DNA-shape protein recognition and supercoiling: The case of IHF
Computational and Structural Biotechnology Journal, 2022 The integration host factor (IHF) is a prominent example of indirect readout as it imposes one of the strongest bends on relaxed linear DNA. However, the relation between IHF and torsionally constrained DNA, as occurs physiologically, remains unclear. By
George D. Watson +3 more
doaj +3 more sources
Structural diversity of supercoiled DNA [PDF]
Nature Communications, 2015 By regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism. Despite its importance, however, much about supercoiled DNA (positively supercoiled DNA, in particular) remains unknown.
A Amzallag +55 more
core +5 more sources
Supercoiled DNA: Structure [PDF]
, 2005 Supercoiling is introduced into DNA molecules when the double helix is twisted around its own axis in three‐dimensional space. Experimental techniques that are sensitive to molecular shape can be used to analyse the topological states of DNA, but the approaches used most successfully are high-speed centrifugation, high-resolution microscopy and gel ...
Bowater, RP
core +6 more sources
DNA Supercoiling Measurement in Bacteria. [PDF]
Methods Mol Biol, 2018 DNA supercoiling plays critical roles in several essential DNA metabolic pathways, such as replication, transcription and recombination. Typically plasmid DNA molecules are used to measure DNA supercoiling status inside bacterial cells. In this chapter, we describe how to isolate plasmid DNA molecules from E.
Liu Y, Hua ZC, Leng F.
europepmc +4 more sources
Regulation of DNA supercoiling in Escherichia coli : Genetic basis of a compensatory mutation in DNA gyrase [PDF]
FEBS Letters, 1989 Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a topA deletion mutant of Escherichia coli (DM800) that has a ...
Wang +13 more
exaly +7 more sources